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421“…SIGNIFICANCE: The firefly enzyme luciferase has been used in a wide range of biological assays, including bioluminescence imaging of adenosine triphosphate (ATP). …”
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422“…An operative standard was established including fluorescence angiography (Firefly®). Technical aspects with focus on the characteristics of the da Vinci® X system, operating room setup, and short-term outcomes are discussed.…”
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423“…Hybrid methods composed of fusion of rough set and binary firefly algorithm based on community weak connection mechanism (CWLBGSO), directed acyclic graph (DAG), and Bootstrap structural equation modeling (Bootstrap SEM) are used to carry out empirical analysis of the conceptual model of function mechanism. …”
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424por Belotti, Mattia, El‐Tahawy, Mohsen M. T., Yu, Li‐Juan, Russell, Isabella C., Darwish, Nadim, Coote, Michelle L., Garavelli, Marco, Ciampi, Simone“…Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst—luciferase. …”
Publicado 2022
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425por Shao, Fangyang, Ci, Lei, Shi, Jiahao, Fang, Fei, Yan, Bowen, Liu, Xijun, Yao, Xiangyu, Zhang, Mengjie, Yang, Hua, Wang, Zhugang, Fei, Jian“…To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents, we establish a transgenic mouse line. The firefly luciferase gene is incorporated into the mouse genome and driven by the endogenous MCP-1 promoter. …”
Publicado 2022
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427por Carrio Argos, Fernando“…The CPM has been designed as an AMC form-factor board equipped with 8 Samtec FireFly modules for the on-detector communication, a Xilinx Kintex UltraScale FPGA for data acquisition and processing, a Xilinx Artix 7 FPGA for slow control and monitoring, and other subsystems to generate high-quality clocks for the FPGAs and communications.…”
Publicado 2020
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428por Chorich, Lynn P, Diamond, Michael P, Hall, Janet E, Korach, Kenneth S, Layman, Lawrence C, Li, Yin, Liu, Haitao, Roman, Robert A“…A dual luciferase reporter assay was used to compare relative Firefly and Renilla luciferase activity between WT ESR1, p.Thr313Met, and p.Glu375His plasmids using a luminometer. …”
Publicado 2022
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429por Ali, Muhammad Umair, Hussain, Shaik Javeed, Zafar, Amad, Bhutta, Muhammad Raheel, Lee, Seung Won“…Eight metaheuristic optimization algorithms, namely, the marine predator algorithm, atom search optimization algorithm (ASOA), Harris hawks optimization algorithm, butterfly optimization algorithm, whale optimization algorithm, grey wolf optimization algorithm (GWOA), bat algorithm, and firefly algorithm, are used to evaluate the classification performance using a support vector machine (SVM)-based cost function. …”
Publicado 2023
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430por Sasaki-Tanaka, Reina, Shibata, Toshikatsu, Moriyama, Mitsuhiko, Kogure, Hirofumi, Hirai-Yuki, Asuka, Okamoto, Hiroaki, Kanda, Tatsuo“…In the present study, we established HuhT7-HAV/Luc cells, which are HuhT7 cells stably expressing the HAV HM175-18f genotype IB subgenomic replicon RNA harboring the firefly luciferase gene. This system was made by using a PiggyBac-based gene transfer system that introduces nonviral transposon DNA into mammalian cells. …”
Publicado 2023
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431“…The intracellular ATP levels were measured by the firefly luciferase-based bioluminescence method. It was found that the antimicrobial activity of conjugated bile acids against the strains of lactic acid bacilli is strain-specific, and glycochenodeoxycholic acid showed significantly greater antimicrobial activity than taurochenodeoxycholic acid against the strains of lactic acid bacilli. …”
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432“…Here, we used N. benthamiana leaves and BY2 suspension cells to quantitatively characterize a library of plant promoters by transient expression of firefly/Renilla luciferase. We validated the dual-luciferase reporter system by examining the correlation between reporter protein and mRNA levels. …”
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433“…A fragment encompassing the gag/pol frameshift region of HIV-1 was inserted in the beginning of the coding sequence of a reporter gene coding for the firefly luciferase, such that expression of luciferase requires a –1 frameshift. …”
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434“…METHODS AND RESULTS: Mice were inoculated with recombinant lambda phage containing a mammalian expression cassette encoding firefly luciferase (luc). Efficient, dose-dependent in vivo luc expression was detected, which peaked within 24 h of delivery and declined to undetectable levels within a week. …”
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435Publicado 1988“…A tripeptide of the sequence Ser-Lys/His-Leu is present in each of these targeting signals as well as in the peroxisomal targeting signal identified in firefly luciferase (Gould, S.J., G.-A. Keller, and S. …”
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436“…To investigate translation re-initiation in Escherichia coli, we constructed a di-cistronic reporter in which a firefly luciferase gene was linked to a chloramphenicol acetyltransferase gene using a segment of the translationally coupled geneV–geneVII intercistronic region from M13 phage. …”
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437“…We investigated the gene-to-protein relationship of firefly luciferase under the T7/E.Coli-extract expression system, as well as transcription-only reactions with T7 RNA polymerase, and found both systems to be highly sensitive to brush density, conformation, and orientation. …”
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438por Bartlett, Joanne G, Alves, Sílvia C, Smedley, Mark, Snape, John W, Harwood, Wendy A“…Results of large scale experiments utilising the luc (firefly luciferase) gene as a reporter are described. …”
Publicado 2008
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439“…An in vivo reporter gene assay using each 5′-UTR upstream of the firefly luciferase gene showed that the 5′-UTRs for PPAR-γ1, -γ2, and -γ4 enhanced translation, whereas the 5′-UTRs for PPAR-γ5 and -γ7 inhibited translation. …”
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440por Vallejos, Maricarmen, Ramdohr, Pablo, Valiente-Echeverría, Fernando, Tapia, Karla, Rodriguez, Felipe E., Lowy, Fernando, Huidobro-Toro, J. Pablo, Dangerfield, John A., López-Lastra, Marcelo“…The 5′-UTR of the full-length mRNA derived from the infectious, complete MMTV genome was cloned into a dual luciferase reporter construct containing an upstream Renilla luciferase gene (RLuc) and a downstream firefly luciferase gene (FLuc). In rabbit reticulocyte lysate, the MMTV 5′-UTR was capable of driving translation of the second cistron. …”
Publicado 2010
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