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561por Sakakura, Noriaki, Nakada, Takeo, Takahashi, Yusuke, Suzuki, Ayumi, Shinohara, Shuichi, Kuroda, Hiroaki“…To identify segmental boundaries, we administered indocyanine green intravenously and used the robotic fluorescence imaging system (Firefly). In all procedures, cranial-side intrathoracic structures, which are often hidden in the conventional look-up-view method, were well visualized. …”
Publicado 2022
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562“…Here, we used total internal reflection fluorescence (TIRF) microscopy and single-molecule fluorescence resonance energy transfer (smFRET) to temporally observe the conformational changes that occur to individual firefly luciferase proteins as they are folded by the bacterial Hsp70 system. …”
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563“…We established a stable firefly luciferase reporter gene knock-in U2-OS cell line by CRISPR/Cas9 to screen circadian-rhythm-disrupting pollutants. …”
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564por Gurumoorthy, Sasikumar, Subhash, Parimella, Pérez de Prado, Rocio, Wozniak, Marcin“…The Packet Delivery Ratio (PDR) for 100 nodes is 0.98 at 500 rounds, which is high when compared to Grey Wolf Optimization (GWO), Multi-Objective Fractional Particle Lion Algorithm (MOFPL), Sparrow Search Algorithm (SSA), Bald Eagle Search optimization (BES), Rider Optimization (ROA), Hunger Games Search (HGS), Shark Smell Optimization (SSO), Rider-Cat Swarm Optimization (RCSO), and Firefly Cyclic Randomization (FCR) methods.…”
Publicado 2022
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565Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cellspor Vysochinskaya, Vera, Shishlyannikov, Sergey, Zabrodskaya, Yana, Shmendel, Elena, Klotchenko, Sergey, Dobrovolskaya, Olga, Gavrilova, Nina, Makarova, Darya, Plotnikova, Marina, Elpaeva, Ekaterina, Gorshkov, Andrey, Moshkoff, Dmitry, Maslov, Mikhail, Vasin, Andrey“…Liposomes 2X3-DOPE (1:3) provided the superior delivery of both mRNA coding firefly luciferase and mRNA-eGFP into BHK-21 cells and A549 cells, compared with commercial Lipofectamine MessengerMax.…”
Publicado 2022
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566“…Our method is compared with different optimization methods such as Ant Colony Optimization (ACO), Firefly Optimization (FFO), and Enhanced Flying Ant Colony Optimization (EFACO). …”
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568por Shakiba, Yasmin, Vorobyev, Pavel O., Naumenko, Victor A., Kochetkov, Dmitry V., Zajtseva, Ksenia V., Valikhov, Marat P., Yusubalieva, Gaukhar M., Gumennaya, Yana D., Emelyanov, Egor A., Semkina, Alevtina S., Baklaushev, Vladimir P., Chumakov, Peter M., Lipatova, Anastasia V.“…In this study, to enhance the antitumor efficacy of the thymidine kinase-deficient vaccinia virus (VV, Lister strain), recombinant variants encoding bacterial flagellin (subunit B) of Vibrio vulnificus (LIVP-FlaB-RFP), firefly luciferase (LIVP-Fluc-RFP) or red fluorescent protein (LIVP-RFP) were developed. …”
Publicado 2023
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569por Zerouali, Bilel, Santos, Celso Augusto Guimarães, de Farias, Camilo Allyson Simões, Muniz, Raul Souza, Difi, Salah, Abda, Zaki, Chettih, Mohamed, Heddam, Salim, Anwar, Samy A., Elbeltagi, Ahmed“…This study proposes a multilayer perceptron (MLP) network optimized by Genetic Algorithm (GA), Particle Swarm Optimization (PSO), Firefly Algorithm (FFA), and Teleconnection Pattern Indices - such as North Atlantic Oscillation (NAO), Southern Oscillations (SOI), Western Mediterranean Oscillation (WeMO), and Mediterranean Oscillation (MO) - to model monthly rainfalls at the Sebaou River basin (Northern Algeria). …”
Publicado 2023
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570por Aqeel, Ibrahim, Khormi, Ibrahim Mohsen, Khan, Surbhi Bhatia, Shuaib, Mohammed, Almusharraf, Ahlam, Alam, Shadab, Alkhaldi, Nora A.“…For instance, while the Artificial Bee Colony (ABC), Gravitational Search Algorithm (GSA), and Whale Defense Algorithm with Firefly Algorithm (WD-FA) techniques attain average throughputs of 58.247 Kbps, 59.957 Kbps, and 60.819 Kbps, respectively, the CHROA model achieves an average throughput of 70.122 Kbps. …”
Publicado 2023
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571“…Twelve different classifiers, such as PCA, EM, logistic regression, GMM, BLDC, firefly clusters, harmonic search, detrend fluctuation analysis, PAC Bayesian learning, KNN-PAC Bayesian, softmax discriminant classifier, and detrend with SDC were utilized to detect CVD from dimensionally reduced PPG signals. …”
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572por Pan, Yonghua, Rohde, Michelle, Zeitler, Jennifer, Namburi, Sai Valli Srujana, Cao, Liching, Hu, Jing, Meyer, Kathleen, Lu, Yanmei“…To identify critical factors impacting TI assay detection of AAV neutralizing antibodies (NAbs), we created a reporter construct expressing NanoLuc® luciferase (Nluc) that enabled a more sensitive and robust detection of AAV6 NAbs than using firefly luciferase. Assessment of additional factors including multiplicity of infection, cell lines, viral production, and capsid purity revealed the reporter is the major determinant of assay sensitivity impacting NAb detection. …”
Publicado 2023
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573“…We also tested the effects of these DnaJ homologues on folding of guanidine-denatured firefly luciferase. Unexpectedly, dj2, but not dj1, together with hsc70 refolded the protein efficiently. …”
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574“…The serum-induced intracellular calcium peak was essential for subsequent NF-κB activation (measured by real-time imaging of the dynamic p65 and IκBα fluorescent fusion proteins), cyclin D1 (CD1) promoter-directed transcription (measured by real-time luminescence imaging of CD1 promoter-directed firefly luciferase activity), and progression to cell division. …”
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575por Gendron, Karine, Charbonneau, Johanie, Dulude, Dominic, Heveker, Nikolaus, Ferbeyre, Gerardo, Brakier-Gingras, Léa“…CD4+ T cells and 293T cells were transfected with a dual-luciferase construct where the firefly luciferase expression depends upon the HIV-1 frameshift. …”
Publicado 2008
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576por Li, Limei, Fei, Zhaoliang, Ren, Jianke, Sun, Ruilin, Liu, Zhihui, Sheng, Zhejin, Wang, Long, Sun, Xia, Yu, Jun, Wang, Zhugang, Fei, Jian“…To understand the role of IL-1β in disease processes and develop an in vivo screening system for anti-inflammatory drugs, a transgenic mouse line was generated which incorporated the transgene firefly luciferase gene driven by a 4.5-kb fragment of the human IL-1β gene promoter. …”
Publicado 2008
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577“…The 4T1 cell line has been engineered to constitutively express the firefly luciferase gene (luc2). When mice carrying 4T1-luc2 tumors are injected with Luciferin the tumors emit a visual light signal that can be monitored using a sensitive optical imaging system like the IVIS Spectrum. …”
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578“…Here, we show that DNA quadruplexes enhance in vivo MyoD and E-box-driven expression of a firefly luciferase (FL) reporter gene. HEK293 cells were transfected with FL expressing p4RTK-FL vector alone or together with MyoD expressing pEMSV-MyoD plasmid, with quadruplexes of α7 integrin or sarcomeric mitochondrial creatine kinase (sMtCK) muscle gene promoters or with a combination thereof. …”
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579por Canavaci, Adriana M. C., Bustamante, Juan M., Padilla, Angel M., Perez Brandan, Cecilia M., Simpson, Laura J., Xu, Dan, Boehlke, Courtney L., Tarleton, Rick L.“…METHODS: In this work, we describe the development and validation of improved methods to test anti-T. cruzi compounds in vitro and in vivo using parasite lines expressing the firefly luciferase (luc) or the tandem tomato fluorescent protein (tdTomato). …”
Publicado 2010
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580por Deleavey, Glen F., Watts, Jonathan K., Alain, Tommy, Robert, Francis, Kalota, Anna, Aishwarya, Veenu, Pelletier, Jerry, Gewirtz, Alan M., Sonenberg, Nahum, Damha, Masad J.“…Effective patterns of modification were chosen based on screens using two sequences targeting firefly luciferase. We then applied the most effective duplex designs to the knockdown of the eIF4E binding proteins 4E-BP1 and 4E-BP2. …”
Publicado 2010
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