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Mostrando 581 - 600 Resultados de 1,123 Para Buscar '"Firefly"', tiempo de consulta: 0.18s Limitar resultados
  1. 581
    por Boellmann, Frank, Thomas, Russell S.
    Publicado 2010
    “…In the primary screen, a total of 13724 full-length genes in mammalian expression vectors were individually co-transfected into human embryonic kidney cells together with a human HSP70B promoter driving firefly luciferase. A subset of the full-length genes that showed significant activation in the primary screen were then evaluated for their ability to hyper-activate the HSP70B under heat shock conditions. …”
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    The Identification of Protein Kinase C Iota as a Regulator of the Mammalian Heat Shock Response Using Functional Genomic Screens
  2. 582
    “…Immunohistochemistry staining of tumour sections from mice treated with PSGL-1-retargeted virus showed a co-localisation of firefly luciferase with CD31 suggesting selective endothelial targeting. …”
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    Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins
  3. 583
    “…Luciferase-harboring vectors (pGL3) were transferred into liposomes and the transfection efficiencies were determined by luciferase assay. Firefly luciferase is one of most popular reporter genes often used to measure the efficiency of gene transfer in vivo and in vitro. …”
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    Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
  4. 584
    “…METHODS: We have used strains that express the bacterial lux operon and therefore do not require exogenous substrate to produce light, as well as strains expressing the firefly luciferase that need luciferin substrate. …”
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    Rapid measurement of antituberculosis drug activity in vitro and in macrophages using bioluminescence
  5. 585
    “…When tested in a cell-free reconstituted transcription–translation assay the synthesis of a full-length protein, firefly luciferase, was notably slower with JE105 70S ribosomes and 50S subunits. …”
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    Bacterial ribosome requires multiple L12 dimers for efficient initiation and elongation of protein synthesis involving IF2 and EF-G
  6. 586
    “…When KM-Luc/GFP malignant fibrous histiocytoma-like cells stably expressing the firefly luciferase gene were injected into the SiLN, metastasis could be detected in the proper-ALN within 3 to 9 days, using in vivo bioluminescence imaging. …”
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    Mouse Model of Lymph Node Metastasis via Afferent Lymphatic Vessels for Development of Imaging Modalities
  7. 587
    “…Using a transgenic reporter line that expresses firefly luciferase under the control of the murine Gfap promoter (Gfap-luc), we found that luciferase activity reflected the regional CNS (central nervous system) variability of Gfap mRNA in Gfap(+/+) mice, and increased in mice containing a point mutation in Gfap that mimics a common human mutation in AxD (R239H in the human sequence, and R236H in the murine sequence). …”
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    GFAP expression as an indicator of disease severity in mouse models of Alexander disease
  8. 588
    “…Multimodality imaging using noncytotoxic triple fusion (TF) reporter genes is an important application for cell-based tracking, drug screening, and therapy. The firefly luciferase (fl), monomeric red fluorescence protein (mrfp), and truncated herpes simplex virus type 1 thymidine kinase SR39 mutant (ttksr39) were fused together to create TF reporter gene constructs with different order. …”
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    Rational Design of a Triple Reporter Gene for Multimodality Molecular Imaging
  9. 589
    “…METHODS: U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. …”
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    Targeting Orthotopic Glioma in Mice with Genetically Engineered Salmonella typhimurium
  10. 590
    por Geng, Deyu, Zhang, Zhixia, Guo, Huarong
    Publicado 2012
    “…Here we report the development of a fish cell biosensor system for high throughput genotoxicity detection of new drugs, by stably integrating two reporter plasmids of pGL(3)-p21-luc (human p21 promoter linked to firefly luciferase) and pRL-CMV-luc (CMV promoter linked to Renilla luciferase) into marine flatfish flounder gill (FG) cells, referred to as p21FGLuc. …”
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    Development of a Fish Cell Biosensor System for Genotoxicity Detection Based on DNA Damage-Induced Trans-Activation of p21 Gene Expression
  11. 591
    “…Adenosine triphosphate (ATP) level in the midbrain tissue was detected by firefly luciferase method. MitoK(ATP) channel subunits SUR1 and Kir6.2 mRNA and protein expressions were tested by real-time PCR (RT-PCR) and Western blot. …”
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    Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson's Disease
  12. 592
    “…Importantly, UCN acts as a transducer to sensitize ZnO and trigger the generation of cytotoxic reactive oxygen species (ROS) to induce cancer cell death. We also present a firefly luciferase (FLuc) reporter gene based molecular biosensor (ARE-FLuc) to measure the antioxidant signaling response activated in cells during the release of ROS in response to the exposure of CSNPs under 980 nm NIR light. …”
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    Core – shell upconversion nanoparticle – semiconductor heterostructures for photodynamic therapy
  13. 593
    “…These molecules were transfected into Huh7.5 cells that stably harboured an HCV subgenomic replicon expressing a firefly luciferase/neoR reporter (SGR-Feo-JFH-1) and were also tested on HCVcc-infected cells. …”
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    Evaluation of Canonical siRNA and Dicer Substrate RNA for Inhibition of Hepatitis C Virus Genome Replication – A Comparative Study
  14. 594
    “…RESULTS: To obtain functional mitochondria, S. cerevisiae cells were lysed with zymolyase followed by two-step, low- then high-speed centrifugation. Using a firefly luciferin-luciferase assay, the ATP synthetic activity of the mitochondria was determined. …”
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    Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity
  15. 595
    “…Using 9464D cells stably expressing firefly luciferase, enhanced IA tumor growth could be confirmed using bioluminescence. …”
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    Intra-adrenal murine TH-MYCN neuroblastoma tumors grow more aggressive and exhibit a distinct tumor microenvironment relative to their subcutaneous equivalents
  16. 596
    “…NK cells were tagged with enhanced firefly luciferase (effluc) using a recombinant retrovirus transfection (NKF). …”
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    Potential Therapeutic Effect of Natural Killer Cells on Doxorubicin-Resistant Breast Cancer Cells In Vitro
  17. 597
    “…The cells were stably transfected with the gene coding for firefly luciferase. Tumour development was monitored using PET and BLI and in two subgroups, on days 3 and 4 or on days 6 and 7 after tumour cell inoculation. …”
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    Non-invasive imaging of implanted peritoneal carcinomatosis in mice using PET and bioluminescence imaging
  18. 598
    “…RESULTS: An intron containing firefly luciferase gene was used as a reporter for agroinfiltration. …”
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    Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana
  19. 599
    “…In this study, we have investigated the role of wobble uridine modifications in reading frame maintenance, using either the Renilla/Firefly luciferase bicistronic reporter system or a modified Ty1 frameshifting site in a HIS4A::lacZ reporter system. …”
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    The role of wobble uridine modifications in +1 translational frameshifting in eukaryotes
  20. 600
    “…METHODS: The in vitro (anti)estrogenic activity of norfluoxetine was assessed using a firefly luciferase reporter construct in the T47D-Kbluc breast cancer cell line. …”
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    In vitro modulation of estrogen receptor activity by norfluoxetine
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