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741“…We also find that fluoroquinolones, such as ciprofloxacin, can substantially increase the expression of the firefly luciferase under the control of the P(gyrA) coupled to a divergent IPTG-inducible promoter in the presence of IPTG. …”
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742“…We sought to test if the kinetics of firefly luciferase oxidizing luciferin, the reaction widely used as a read-out in various biochemical assays, is also affected by an elevated deuterium content. …”
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743por Ngo, Phuong-Thao Thi, Hoang, Nhat-Duc, Pradhan, Biswajeet, Nguyen, Quang Khanh, Tran, Xuan Truong, Nguyen, Quang Minh, Nguyen, Viet Nghia, Samui, Pijush, Tien Bui, Dieu“…The SAR imagery is used to detect flash flood inundation areas, whereas the new machine learning technique, which is a hybrid of the firefly algorithm (FA), Levenberg–Marquardt (LM) backpropagation, and an artificial neural network (named as FA-LM-ANN), was used to construct the prediction model. …”
Publicado 2018
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744por Bofinger, Robin, Zaw‐Thin, May, Mitchell, Nicholas J., Patrick, P. Stephen, Stowe, Cassandra, Gomez‐Ramirez, Ana, Hailes, Helen C., Kalber, Tammy L., Tabor, Alethea B.“…The biophysical properties of the lipopolyplexes and their transfection efficiencies in a basal‐like breast cancer cell line were investigated using plasmid DNA bearing genes for the expression of firefly luciferase and green fluorescent protein. …”
Publicado 2018
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745por Kleinovink, Jan Willem, Mezzanotte, Laura, Zambito, Giorgia, Fransen, Marieke F., Cruz, Luis J., Verbeek, J. Sjef, Chan, Alan, Ossendorp, Ferry, Löwik, Clemens“…Here, we describe the design and generation of a transgenic mouse in which all T cells constitutively express green-emitting click-beetle luciferase (CBG99) while expression of the red-emitting firefly luciferase (PpyRE9) is induced by Nuclear Factor of Activated T cells (NFAT) such as during T cell activation, which allows multicolor bioluminescence imaging of T cell location and function. …”
Publicado 2019
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746por Liu, Ya-Hui, Wu, Pei-Hua, Kang, Chih-Cheng, Tsai, Yau-Sheng, Chou, Chuan-Kai, Liang, Chung-Tiang, Wu, Jiunn-Jong, Tsai, Pei-Jane“…Therefore, we used a bioluminescent GAS strain and reporter mice carrying firefly luciferase under transcriptional control of the nuclear factor-kappa B (NF-κB) promoter to concurrently monitor the host immune response and bacterial burden in a single mouse. …”
Publicado 2019
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747por Li, Xiaoxia, Xie, Jing, Zhai, Yue, Fang, Tengjiaozi, Rao, Nanquan, Hu, Shuang, Yang, Liping, Zhao, Yuming, Wang, Yixiang, Ge, Lihong“…SHEDs were labeled with firefly luciferase for in vivo tracking by bioluminescent imaging and then transplanted into the subretinal space of mice. …”
Publicado 2019
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748por Sônego, Fabiane, Bouccara, Sophie, Pons, Thomas, Lequeux, Nicolas, Danckaert, Anne, Tinevez, Jean-Yves, Alam, Israt S., Shorte, Spencer L., Tournebize, Régis“…Here, we applied FUEL to tumor detection in two different subcutaneous tumor models: the auto-luminescent human embryonic kidney (HEK293) cell line and the murine B16-F10 melanoma cell line previously transfected with a plasmid encoding the Luc2 firefly luciferase. Tumor size and bioluminescence were measured over time and tumor vascularization characterized. …”
Publicado 2019
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749por Ghazvinian, Hamidreza, Mousavi, Sayed-Farhad, Karami, Hojat, Farzin, Saeed, Ehteram, Mohammad, Hossain, Md Shabbir, Fai, Chow Ming, Hashim, Huzaifa Bin, Singh, Vijay P., Ros, Faizah Che, Ahmed, Ali Najah, Afan, Haitham Abdulmohsin, Lai, Sai Hin, El-Shafie, Ahmed“…In addition, different models have been tested for this prediction, namely, the M5 tree model (M5T), genetic programming (GP), SVR integrated with four different optimization algorithms SVR-PSO, SVR-IPSO, Genetic Algorithm (SVR-GA), FireFly Algorithm (SVR-FFA) and the multivariate adaptive regression (MARS) model. …”
Publicado 2019
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750por Firuzi, Omidreza, Che, Pei Pei, El Hassouni, Btissame, Buijs, Mark, Coppola, Stefano, Löhr, Matthias, Funel, Niccola, Heuchel, Rainer, Carnevale, Ilaria, Schmidt, Thomas, Mantini, Giulia, Avan, Amir, Saso, Luciano, Peters, Godefridus J., Giovannetti, Elisa“…In novel 3D-spheroid co-cultures of cyan fluorescence protein (CFP)-firefly luciferase (Fluc)-expressing primary human PDAC cells and green fluorescence protein (GFP)-expressing immortalized PSCs, PDAC cell growth and chemosensitivity were examined by luciferase assay, while spheroids’ architecture was evaluated by confocal microscopy. …”
Publicado 2019
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751por de Lange, Iris M., Weuring, Wout, van ‘t Slot, Ruben, Gunning, Boudewijn, Sonsma, Anja C. M., McCormack, Mark, de Kovel, Carolien, van Gemert, Lisette J. J. M., Mulder, Flip, van Kempen, Marjan J. A., Knoers, Nine V. A. M., Brilstra, Eva H., Koeleman, Bobby P. C.“…METHODS: Five different SCN1A promoter‐haplotypes were functionally assessed in SH‐SY5Y cells using Firefly and Renilla luciferase assays. The SCN1A promoter region was analyzed in a cohort of 143 participants with SCN1A pathogenic variants. …”
Publicado 2019
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752“…Purifications were prepared from healthy plants and plants that had been infiltrated with Agrobacterium tumefaciens carrying infectious cDNA of Potato virus A (PVA) or firefly luciferase gene, referred to here as PVA‐ or Agrobacterium‐infected plants, respectively. …”
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753“…Genetically modified plasmids containing the merR gene were used to design biosensors. Firefly luciferase (LucFF) and emerald green fluorescent protein (EmGFP) genes were separately introduced as a reporter. …”
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754por Malek-Adamian, Elise, Fakhoury, Johans, Arnold, Amy E., Martínez-Montero, Saúl, Shoichet, Molly S., Damha, Masad J.“…In this study, we explore the effect of a library of 2′-, 4′-, and 2′,4′-modified uridine nucleosides and their impact on silencing firefly luciferase and on down-regulated in renal cell carcinoma (DRR) gene targets. …”
Publicado 2019
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755por Weiner, Zachary P., Salzer, Johanna S., LeMasters, Elizabeth, Ellison, James A., Kondas, Ashley V., Morgan, Clint N., Doty, Jeffery B., Martin, Brock E., Satheshkumar, Panayampalli Subbian, Olson, Victoria A., Hutson, Christina L.“…In this study, we used recombinant MPXV containing the firefly luciferase gene (luc) and in vivo imaging technology to characterize MPXV pathogenesis in the black-tailed prairie dog in real time. …”
Publicado 2019
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756por Wei, Rui, Jiang, Guiying, Lv, MengQin, Tan, Songwei, Wang, Xueqian, Zhou, Ying, Cheng, Teng, Gao, Xueqin, Chen, Xi, Wang, Wei, Zou, Chenming, Li, Fei, Ma, Xiangyi, Hu, Junbo, Ma, Ding, Luo, Danfeng, Xi, Ling“…Human cervical cancer HeLa cells engineered by firefly luciferase were injected into the right hocks of BALB/c nude mice to develop the SLN metastasis model. …”
Publicado 2019
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757“…To evaluate the effects of siRNA-mediated silencing, we established cell lines expressing a bicistronic luciferase reporter plasmid, which contained an FMDV-IRES element between the Renilla and firefly luciferase genes. The designed siRNA inhibited FMDV-IRES-mediated translation in a concentration-dependent manner. …”
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758por Taschauer, Alexander, Polzer, Wolfram, Alioglu, Fatih, Billerhart, Magdalena, Decker, Simon, Kittelmann, Theresa, Geppl, Emanuela, Elmenofi, Salma, Zehl, Martin, Urban, Ernst, Sami, Haider, Ogris, Manfred“…Polyplexes applied via intratracheal aerosolization were well tolerated and resulted in measurable transgene activity of the reporter gene firefly luciferase in tumor areas by bioluminescence imaging (BLI). …”
Publicado 2019
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759por Kallmeyer, Karlien, André‐Lévigne, Dominik, Baquié, Mathurin, Krause, Karl‐Heinz, Pepper, Michael S., Pittet‐Cuénod, Brigitte, Modarressi, Ali“…ASCs were transduced to express both firefly luciferase (Fluc) and green fluorescent protein to enable tracking by bioluminescence imaging and immunohistological analysis. …”
Publicado 2019
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760por Chen, Yi-Ning, Hsu, Hsiao-Chin, Wang, Sheng-Wei, Lien, Hao-Chiang, Lu, Hsin-Ti, Peng, Sheng-Kai“…Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of Scotophilus bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. …”
Publicado 2019
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