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801por Liu, Yanfei, Lv, Guowen, Yang, Yaqi, Ma, Kangxun, Ren, Xiaolin, Li, Mingjun, Liu, Zhande“…The results of the dual-luciferase reporter, firefly luciferase complementation, yeast two-hybrid and co-immunoprecipitation assays showed that AcMADS68 could interact with both AcMYBF110 and AcMYB123 but not with AcbHLH1, thereby co-regulating anthocyanin biosynthesis by promoting the activation of the target genes, including AcANS, AcF3GT1, and AcGST1. …”
Publicado 2022
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802por Koch, Jana, Beil, Julia, Berchtold, Susanne, Mönch, Dina, Maaß, Annika, Smirnow, Irina, Schenk, Andrea, Carter, Mary E., Kloker, Linus D., Leibold, Tobias, Renner, Philipp, Dahlke, Marc-H., Lauer, Ulrich M.“…Human HT-29 cancer cells stably expressing marker genes GFP and firefly luciferase (GFP/luc) were cultured on human peritoneum and infected with two prototypic oncolytic viruses (GLV-0b347 and MeV-DsRed). …”
Publicado 2023
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803Publicado 1997Tabla de Contenidos: “…Choe -- The evolution of water strider mating systems : causes and consequences of sexual conflicts / Göran Arnqvist -- Multiple mating, sperm competition, and cryptic female choice in the leaf beetles (Coleoptera : Chrysomelidae) / Janis L. Dickinson -- Firefly mating ecology, selection and evolution / James E. …”
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804por Su, Yichi, Walker, Joel R., Hall, Mary P., Klein, Mark A., Wu, Xiang, Encell, Lance P., Casey, Kerriann M., Liu, Lan Xiang, Hong, Guosong, Lin, Michael Z., Kirkland, Thomas A.“…CFz paired with Antares luciferase produces greater than 20-fold more signal from the brain than the standard combination of d-luciferin with firefly luciferase. At standard doses, Antares–CFz matches AkaLuc–AkaLumine/TokeOni in brightness, while occasional higher dosing of CFz can be performed to obtain threefold more signal. …”
Publicado 2023
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805“…The aim of this study was to investigate the effectiveness of indocyanine green (ICG) fluorescence as a new method for the visual identification of abnormal hyperfunctioning parathyroid glands in robot-assisted parathyroidectomy using Firefly(TM) technology. We also aimed to conduct a comparative analysis between robot-assisted parathyroidectomy and conventional focused parathyroidectomy in order to identify clinical differences between the two surgical approaches. …”
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806por Takahashi, Junji, Yoshida, Masashi, Ohdaira, Hironori, Nakaseko, Yuichi, Nakashima, Keigo, Kamada, Teppei, Suzuki, Norihiko, Sato, Takayuki, Suzuki, Yutaka“…However, this clip is difficult to observe with the Firefly imaging system equipped with the da Vinci® surgical system. …”
Publicado 2023
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807por Mujawar, Aaiyas, Phadte, Pratham, Palkina, Ksenia A., Markina, Nadezhda M., Mohammad, Ameena, Thakur, Bhushan L., Sarkisyan, Karen S., Balakireva, Anastasia V., Ray, Pritha, Yamplosky, Ilia, De, Abhijit“…Luz enzyme or its unique 3OH-hispidin substrate was found to be non-cross-reacting with commonly used luciferase reporters such as Firefly (FLuc2), Renilla (RLuc), or nano-luciferase (NLuc). …”
Publicado 2023
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808por Ogawa, Takumi, Kato, Kanae, Asuka, Harue, Sugioka, Yumi, Mochizuki, Tomofumi, Nishiuchi, Takumi, Miyahara, Taira, Kodama, Hiroaki, Ohta, Daisaku“…In this study, we prepared a hetero-transgraft line composed of non-GM tomato scion and GM-tobacco rootstock expressing firefly luciferase. We also prepared a homograft line (both rootstock and scion are from non-GM tomato) and a heterograft line (non-GM tobacco rootstock and non-GM tomato scion). …”
Publicado 2023
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809por Zhang, Zuying, Tao, Liu, Gao, Lingling, Gao, Yadi, Suo, Jinwei, Yu, Weiyu, Hu, Yuanyuan, Wei, Chunyan, Farag, Mohamed A, Wu, Jiasheng, Song, Lili“…Bimolecular fluorescence complementation, firefly luciferase complementation imaging, co-immunoprecipitation, and GST pull-down assays confirmed a direct protein–protein interaction between TgbHLH95 and TgbZIP44 in vivo and in vitro, and in combination these proteins induced the TgGPPS promoter up to 4.7-fold in transactivation assays. …”
Publicado 2023
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810por Miyabara, Hitomi, Hirano, Ryuichiro, Watanabe, Shigeaki, Soriano, John Clyde Co, Watanabe, Hitomi, Kuchimaru, Takahiro, Kitada, Nobuo, Kadonosono, Tetsuya, Maki, Shojiro A., Kondoh, Gen, Kizaka‐Kondoh, Shinae“…We constructed the HIF activity‐monitoring reporter gene hypoxia‐response element‐Venus‐Akaluc (HVA) that expresses the green fluorescent protein Venus and modified firefly luciferase Akaluc in a HIF activity‐dependent manner, and created transgenic mice harboring HVA transgene (HVA‐Tg). …”
Publicado 2023
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811por Yao, Wenjian, Cui, Xiaohai, Peng, Haodong, Zhang, Yongkang, Jia, Xiangbo, Wu, Sen, Zhao, Jian“…The mechanism of action of IDO1-regulation EC was explored through Firefly luciferase & Renilla luciferase activity reporter, chromatin immunoprecipitation (ChIP) and immunofluorescence (IF) assays. …”
Publicado 2023
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812Publicado 1995“…Although the COOH-terminal KKL of human AGT was shown to be necessary for its peroxisomal import, this tripeptide was unable to direct the peroxisomal import of the bona fide peroxisomal protein firefly luciferase or the reporter protein bacterial chloramphenicol acetyltransferase. …”
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813por Rosenstiel, Philip, Huse, Klaus, Franke, Andre, Hampe, Jochen, Reichwald, Kathrin, Platzer, Cornelia, Roberts, Roland G, Mathew, Christopher G, Platzer, Matthias, Schreiber, Stefan“…Using the different 5' UTR splice forms fused to a firefly luciferase (LUC) reporter we demonstrate a rapamycin-sensitive inhibitory effect of the uORFs on translation efficacy. …”
Publicado 2007
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814por Dougherty, Christopher J., Smith, George W., Dorey, C. Kathleen, Prentice, Howard M., Webster, Keith A., Blanks, Janet C.“…For RPE-specific expression, the HRSE and a (6x) HRE (hypoxia responsive element) oligomer were ligated upstream of the Rpe65 promoter in a pGL3 firefly luciferase plasmid (pGL3-HRSE-6xHRE-Rpe65). …”
Publicado 2008
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815por Cenni, B, Kim, H-K, Bubley, G J, Aebi, S, Fink, D, Teicher, B A, Howell, S B, Christen, R D“…A comparison was made of the ability of MMR-proficient and -deficient cells to remove cisplatin adducts from their genome and to reactivate a transiently transfected plasmid that had previously been inactivated by cisplatin to express the firefly luciferase enzyme. MMR deficiency due to loss of hMLH1 function did not change the extent of platinum (Pt) accumulation or kinetics of removal from total cellular DNA. …”
Publicado 1999
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816“…Transient transfection assay employing firefly luciferase as a reporter revealed that a ~1 kb sequence upstream of translational start site is sufficient for the functional transcriptional activity of falcipain-1 gene, while falcipain-2, -2' and -3 genes that exist within 12 kb stretch on chromosome 11 require ~2 kb upstream sequences for the expression of reporter luciferase activity. …”
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817por Rinaldi, Gabriel, Morales, Maria E., Cancela, Martín, Castillo, Estela, Brindley, Paul J., Tort, José F.“…In order to extend these approaches to less well studied parasitic worms, we developed a test method for the presence of a viable RNAi pathway by silencing the exogenous reporter gene, firefly luciferase (fLUC). We established the method in the human blood fluke Schistosoma mansoni and then confirmed its utility in the liver fluke Fasciola hepatica. …”
Publicado 2008
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818“…METHODS: The lines were engineered for stable expression of firefly luciferase to allow tracking and quantitation of the cells in vivo. …”
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819“…In vitro, we showed that H-89 decreased the RLuc8 bioluminescence signal but did not compete with coelenterazine-h for the RLuc8 active site, and also did not affect the activity of Firefly luciferase. By contrast, another competitive inhibitor of PKA, KT5720, did not affect the activity of RLuc8. …”
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820por Kines, Kristine J., Rinaldi, Gabriel, Okatcha, Tunika I., Morales, Maria E., Mann, Victoria H., Tort, Jose F., Brindley, Paul J.“…Second, electroporation was employed to introduce mRNA encoding firefly luciferase into eggs. Luciferase activity was detected three hours later, whereas luciferase was not evident in eggs soaked in the mRNA. …”
Publicado 2010
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