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901por Sultana, Nishat, Hadas, Yoav, Sharkar, Mohammad Tofael Kabir, Kaur, Keerat, Magadum, Ajit, Kurian, Ann Anu, Hossain, Nadia, Alburquerque, Bremy, Ahmed, Sakib, Chepurko, Elena, Zangi, Lior“…Here, we identified that 5′ UTR, from the fatty acid metabolism gene carboxylesterase 1D (Ces1d), enhanced the translation of firefly luciferase (Luc) modRNA by 2-fold in the heart post-myocardial infarction (MI). …”
Publicado 2020
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902por Zhang, Jia Wei, Wang, Xing, Li, Gao Chao, Wang, Dong, Han, Sheng, Zhang, Yao Dong, Luo, Chen Huan, Wang, Hong Wei, Jiang, Wang Jie, Li, Chang Xian, Li, Xiang Cheng“…Dual-luciferase reporter assay revealed that co-transfection of miR-30a-5p significantly inhibited the activity of firefly luciferase reporter carrying the wild-type 3′UTR of SOCS3. …”
Publicado 2020
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903por Wu, Wei, Wang, Miao-miao, Gong, Hui, Liu, Xiao-fen, Guo, Da-long, Sun, Ning-jing, Huang, Jing-wen, Zhu, Qing-gang, Chen, Kun-song, Yin, Xue-ren“…Bimolecular fluorescence complementation and firefly luciferase complementation imaging assays indicated protein–protein interactions between DkNAC9 and DkERF8/16. …”
Publicado 2020
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904“…METHODS: The attenuated vaccinia VG9-Luc, which originated from Chinese vaccinia Tian Tan strain, was constructed to express firefly luciferase for bioluminescence imaging and to disrupt the thymidine kinase gene for promoting tumor specificity. …”
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905por Khatab, Sohrab, Leijs, Maarten J., van Buul, Gerben, Haeck, Joost, Kops, Nicole, Nieboer, Michael, Bos, P. Koen, Verhaar, Jan A. N., Bernsen, Monique, van Osch, Gerjo J. V. M.“…High G proved to be more suitable for production of injectable beads. Firefly luciferase-expressing rat MSC were used to track MSC viability. …”
Publicado 2020
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906por Bozec, Dominique, Sattiraju, Anirudh, Bouras, Alexandros, Jesu Raj, Joe G, Rivera, Daniel, Huang, Yong, Junqueira Alves, Chrystian, Tejero, Rut, Tsankova, Nadejda M, Zou, Hongyan, Hadjipanayis, Constantinos, Friedel, Roland H“…Akaluciferase (Akaluc) is a new BLI system with higher signal strength than standard firefly luciferase (Fluc). Here, we establish Akaluc BLI as a sensitive method for in vivo tracking of glioma expansion. …”
Publicado 2020
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907por Di Pasquale, Giovanni, Perez Riveros, Paola, Tora, Muhibullah, Sheikh, Tayyab, Son, Aran, Teos, Leyla, Grewe, Brigitte, Swaim, William D., Afione, Sandra, Zheng, Changyu, Jang, Shyh-Ing, Shitara, Akiko, Alevizos, Ilias, Weigert, Roberto, Chiorini, John A.“…Persistence and localization of transduction of these AAVs were tested using vectors encoding firefly luciferase, which was detected 6 months after vector administration. …”
Publicado 2020
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908por Rizvi, Fatima, Everton, Elissa, Smith, Anna R., Liu, Hua, Osota, Elizabeth, Beattie, Mitchell, Tam, Ying, Pardi, Norbert, Weissman, Drew, Gouon-Evans, Valerie“…We confirm specific hepatotropism of mRNA-LNP via intravenous injection of firefly luciferase encoding mRNA-LNP, with protein expression lasting about 3 days. …”
Publicado 2021
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909por Talbot, Lindsay Jones, Chabot, Ashley, Funk, Amy, Nguyen, Phuong, Wagner, Jessica, Ross, Aaron, Tillman, Heather, Davidoff, Andrew, Gottschalk, Stephen, DeRenzo, Christopher“…To overcome this roadblock, and also enable real-time imaging of metastatic disease, we took advantage of LM7 OS cells expressing firefly luciferase (LM7.ffLuc). LM7.ffLuc were implanted in a collagen mesh into the tibia of mice, and mice reliably developed orthotopic tumors and lung metastases as judged by bioluminescence imaging and histopathological analysis. …”
Publicado 2021
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910por Bulté, Dimitri, Van Bockstal, Lieselotte, Dirkx, Laura, Van den Kerkhof, Magali, De Trez, Carl, Timmermans, Jean-Pierre, Hendrickx, Sarah, Maes, Louis, Caljon, Guy“…METHODOLOGY/PRINCIPAL FINDINGS: To enable a combined study of the infection dynamics and underlying immunological events for differential in vivo survival, firefly luciferase (PpyRE9) / red fluorescent protein (DsRed) double-reporter strains were generated of MIL-resistant (MIL-R) and syngeneic MIL-sensitive (MIL-S) Leishmania infantum. …”
Publicado 2021
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911por Yang, Bin, Bao, Wenzheng, Zhang, Wei, Wang, Haifeng, Song, Chuandong, Chen, Yuehui, Jiang, Xiuying“…In order to optimize CVODE model, a complex-valued hybrid evolutionary method based on Grammar-guided genetic programming and complex-valued firefly algorithm is presented. CONCLUSIONS: When tested on three real gene expression datasets from E. coli and Human Cell, the experiment results suggest that CVODE model could improve 20–50% prediction accuracy of gene expression data, which could also infer more true-positive regulatory relationships and less false-positive regulations than ordinary differential equation.…”
Publicado 2021
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912por Fiedorowicz, Katarzyna, Wargocka-Matuszewska, Weronika, Ambrożkiewicz, Karolina A., Rugowska, Anna, Cheda, Łukasz, Fiedorowicz, Michał, Zimna, Agnieszka, Drabik, Monika, Borkowski, Szymon, Świątkiewicz, Maciej, Bogorodzki, Piotr, Grieb, Paweł, Hamankiewicz, Paulina, Kolanowski, Tomasz J., Rozwadowska, Natalia, Kozłowska, Urszula, Klimczak, Aleksandra, Kolasiński, Jerzy, Rogulski, Zbigniew, Kurpisz, Maciej“…Bioluminescent imaging (BLI) showed the highest increase in firefly luciferase (fluc) signal intensity in post-infarction heart treated with combination of huSkM and MSCs vs. huSkM alone (p < 0.0001). …”
Publicado 2021
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913por Li, Mengjie, Lin, Ling, Guo, Tingting, Wu, Yujian, Lin, Jiayi, Liu, Yuanyuan, Yang, Ke, Hu, Chenxia“…MDA-MB-231 cells labeled with firefly luciferase were inoculated into the mammary fatty pads of nude mice to establish a transplantation tumor model of human breast cancer. …”
Publicado 2021
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914por Ide, Yutaro, Kitab, Bouchra, Ito, Nobumasa, Okamoto, Riai, Tamura, Yui, Matsui, Takafumi, Sakoda, Yoshihiro, Tsukiyama-Kohara, Kyoko“…To investigate the common host factors associated with these IRESs, we established cell lines expressing a bicistronic luciferase reporter plasmid containing an FMDV-IRES or CSFV-IRES element between the Renilla and firefly luciferase genes. First, we treated FMDV-IRES cells with the French maritime pine extract, Pycnogenol (PYC), and examined its suppressive effect on FMDV-IRES activity, as PYC has been reported to have antiviral properties. …”
Publicado 2022
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915“…CONCLUSION: B (EIA) that is based on the BLEIA method, which applies firefly luciferase luminescence, is more sensitive than stool antigen test kits that are currently marketed in Japan and is very useful in diagnosing H. pylori infection, especially in situations where noninvasive tests are preferred, such as in children.…”
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916por Liu, Lei, Sun, Yanchao, Di, Pengcheng, Cui, Yakun, Meng, Qingchang, Wu, Xiaming, Chen, Yanping, Yuan, Jianhua“…Moreover, immunoprecipitation-mass spectrometry (IP-MS) assay and firefly luciferase complementation imaging (LCI) assay showed that ZmBSK1 could associate with heat shock protein ZmHSP8 and 14-3-3-like protein ZmGF14-6, and their gene expression levels could be significantly induced by NaCl treatment in different maize tissues. …”
Publicado 2022
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917“…The analysis of the promoter deletion series of HRE2 using firefly luciferase and GUS as reporter genes indicated that the −116 to −2 region is responsible for both hypoxia and salt stress responses. …”
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918por Jiao, Chunyang, Gong, Junqing, Guo, Zhujuan, Li, Shuwen, Zuo, Yixin, Shen, Yingbai“…In addition, a yeast two-hybrid assay, firefly luciferase complementation imaging assay, and an in vitro pulldown assay showed that CAM3 interacts with the N-terminus of ACA8, and qRT-PCR showed that some JA-related genes and defense genes expressions were enhanced when treated with linalool in Arabidopsis leaves. …”
Publicado 2022
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919por Americo, Jeffrey L., Cotter, Catherine A., Earl, Patricia L., Liu, Ruikang, Moss, Bernard“…Using a recombinant MVA expressing firefly luciferase for tracking, live imaging revealed luminescence of the respiratory tract of mice within 6 h and persisting for 3 d following intranasal inoculation, whereas luminescence remained at the site of intramuscular vaccination. …”
Publicado 2022
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920por Yamada, Kentaro, Kuribayashi, Koji, Inomata, Naotaka, Noguchi, Kazuko, Kimitsuki, Kazunori, Demetria, Catalino S., Saito, Nobuo, Inoue, Satoshi, Park, Chun‐Ho, Kaimori, Ryo, Suzuki, Motoi, Saito‐Obata, Mariko, Kamiya, Yasuhiko, Manalo, Daria L., Quiambao, Beatriz P., Nishizono, Akira“…Using the serum from a mouse inoculated intramuscularly (i.m.) with 5 × 10(5) focus‐forming units (FFU) of recombinant RABV expressing red firefly luciferase (1088/RFLuc) immediately before symptom onset, two‐dimensional differential gel electrophoresis was conducted, followed by mass spectrometry, and it was confirmed that apolipoprotein A1 (ApoA1) was up‐regulated. …”
Publicado 2021
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