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941por Khan, Tasmiyah, van Brummelen, Anna C, Parkinson, Christopher J, Hoppe, Heinrich C“…METHODS: In vitro cultures of Plasmodium falciparum 3D7 wild-type or firefly luciferase-expressing parasites were incubated with a panel of six anti-malarial compounds for 10 hours and parasite ATP levels or luciferase activity determined at two-hour intervals using luminescence-based reagents. …”
Publicado 2012
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942por Gaikwad, Snehal M., Gunjal, Lata, Junutula, Anitha R., Astanehe, Arezoo, Gambhir, Sanjiv Sam, Ray, Pritha“…A PIK3CA sensor was developed using a bi-fusion reporter from a newly constructed library of bi- and tri-fusion vectors comprising of two mutant far red fluorescent proteins (mcherry/mch and tdTomato/tdt), a mutant firefly luciferase (fluc2), and a PET reporter protein (ttk). …”
Publicado 2013
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943por Duggal, Rohit, Geissinger, Ulrike, Zhang, Qian, Aguilar, Jason, Chen, Nanhai G, Binda, Elena, Vescovi, Angelo L, Szalay, Aladar A“…One of the cell lines, after being transduced with a cDNA encoding firefly luciferase, could be used for real time tumor imaging. …”
Publicado 2013
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944por Kim, Hee-Kyoung, Lee, Seunghoon, Jo, Seong-Mi, McCormick, Susan P., Butchko, Robert A. E., Proctor, Robert H., Yun, Sung-Hwan“…Prior to gene analysis, we constructed a novel luciferase reporter system consisting of a transgenic F. graminearum strain expressing a firefly luciferase gene under control of the promoter for either TRI6 or ZEB2 controlling the biosynthesis of these mycotoxins. …”
Publicado 2013
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945“…Young rhesus monkeys that had been transplanted prenatally with 25×10(6) hPBSC expressing firefly luciferase were assessed with bioluminescence imaging (BLI), then 0.3 mCi of (89)Zr-Df-CD45, which showed the best radiolabeling efficiency, was injected intravenously for PET imaging. …”
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946por Kedziorek, Dorota A., Solaiyappan, Meiyappan, Walczak, Piotr, Ehtiati, Tina, Fu, Yingli, Bulte, Jeff W.M., Shea, Steven M., Brost, Alexander, Wacker, Frank K., Kraitchman, Dara L.“…In the present study, mesenchymal stem cells (MSCs) were transfected with triple fusion reporter gene containing red fluorescent protein, truncated thymidine kinase (SPECT/PET reporter) and firefly luciferase (bioluminescence reporter). Transfected cells were microencapsulated in either unlabeled or perfluorooctylbromide (PFOB) impregnated alginate. …”
Publicado 2013
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947por Zajakina, Anna, Vasilevska, Jelena, Zhulenkovs, Dmitry, Skrastina, Dace, Spaks, Artjoms, Plotniece, Aiva, Kozlovska, Tatjana“…In vivo experiments were performed in a subcutaneous 4 T1 mouse mammary tumor model with different 5-FU doses and an SFV vector encoding firefly luciferase. RESULTS: Infection of 4 T1 cells with SFV prior to 5-FU treatment did not produce a synergistic anti-proliferative effect. …”
Publicado 2014
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948por Guo, Fang, Zhao, Xuesen, Gill, Tina, Zhou, Yan, Campagna, Matthew, Wang, Lijuan, Liu, Fei, Zhang, Pinghu, DiPaolo, Laura, Du, Yanming, Xu, Xiaodong, Jiang, Dong, Wei, Lai, Cuconati, Andrea, Block, Timothy M., Guo, Ju-Tao, Chang, Jinhong“…Our screening platform is a HEK293 cell-based reporter assay where the expression of a firefly luciferase is under the control of a human IFN-β promoter. …”
Publicado 2014
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949por Adamczak, Joanna M., Schneider, Gabriele, Nelles, Melanie, Que, Ivo, Suidgeest, Ernst, van der Weerd, Louise, Löwik, Clemens, Hoehn, Mathias“…We used a transgenic mouse expressing firefly luciferase under the control of the VEGFR2 promotor to non-invasively elucidate the temporal profile of VEGFR2 expression after stroke as a biomarker for VEGF/VEGFR2 signaling. …”
Publicado 2014
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950por Hwang, Do Won, Jin, Yeona, Lee, Do Hun, Kim, Han Young, Cho, Han Na, Chung, Hye Jin, Park, Yunwoong, Youn, Hyewon, Lee, Seung Jin, Lee, Hong J., Kim, Seung U., Wang, Kyu-Chang, Lee, Dong Soo“…F3 cells expressing enhanced firefly luciferase (F3-effLuc) were established through retroviral infection. …”
Publicado 2014
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951por Lepperhof, Vera, Polchynski, Olga, Kruttwig, Klaus, Brüggemann, Chantal, Neef, Klaus, Drey, Florian, Zheng, Yunjie, Ackermann, Justus P., Choi, Yeong-Hoon, Wunderlich, Thomas F., Hoehn, Mathias, Hescheler, Jürgen, Šarić, Tomo“…To assess the survival kinetics of induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) we generated transgenic murine iPSC lines which, in addition to CM-specific expression of puromycin N-acetyl-transferase and enhanced green fluorescent protein (EGFP), also constitutively express firefly luciferase (FLuc) for bioluminescence (BL) in vivo imaging. …”
Publicado 2014
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952por Spronken, Monique I., Short, Kirsty R., Herfst, Sander, Bestebroer, Theo M., Vaes, Vincent P., van der Hoeven, Barbara, Koster, Abraham J., Kremers, Gert-Jan, Scott, Dana P., Gultyaev, Alexander P., Sorell, Erin M., de Graaf, Miranda, Bárcena, Montserrat, Rimmelzwaan, Guus F., Fouchier, Ron A.“…Specifically, enhanced green fluorescent protein (eGFP), far-red fluorescent protein (fRFP), near-infrared fluorescent protein (iRFP), Gaussia luciferase (gLUC) and firefly luciferase (fLUC) were inserted into the PA gene segment of A/PR/8/34 (H1N1). …”
Publicado 2015
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953por Tolstyka, Zachary P., Phillips, Haley, Cortez, Mallory, Wu, Yaoying, Ingle, Nilesh, Bell, Jason B., Hackett, Perry B., Reineke, Theresa M.“…These polymers along with a series of controls were used to form polyplexes with plasmids encoding firefly luciferase behind a strong ubiquitous promoter. …”
Publicado 2015
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954“…We evaluated and compared the IRES-mediated translation activities among cell lines derived from four different animal species using bicistronic luciferase reporter plasmid, which possesses an FMDV-IRES element between Renilla and Firefly luciferase genes. Furthermore, we analyzed the effect of the cellular factors on IRES-mediated translation by silencing the cellular factors using siRNA in both FMDV-susceptible and -insusceptible animal cells. …”
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955por Yang, Xiangyu, Wang, Zhe, Zhang, Fuwu, Zhu, Guizhi, Song, Jibin, Teng, Gao-Jun, Niu, Gang, Chen, Xiaoyuan“…LN metastasis model was developed by hock injection of firefly luciferase engineered human head neck squamous carcinoma cancer UM-SCC-22B cells or human ovarian cancer SKOV-3 cells. …”
Publicado 2017
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956por Zhang, Zheng, Yang, Chao, Shen, Mingzhi, Yang, Ming, Jin, Zhitao, Ding, Liping, Jiang, Wei, Yang, Junke, Chen, Haixu, Cao, Feng, Hu, Taohong“…METHODS: BM-MSCs, isolated from Tg(Fluc-egfp) mice which constitutively express both firefly luciferase (Fluc) and enhanced green fluorescent protein (eGFP), were preconditioned with HPC (1% O(2)) for 12 h, 24 h, 36 h, and 48 h, respectively, followed by 24 h of hypoxia and serum deprivation (H/SD) injury. …”
Publicado 2017
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957“…In combination with real-time in vivo transcriptional profiling using firefly luciferase, we identified the alsT gene as a new member of the GlnR regulon. …”
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958por Li, Peng, Tian, Mingxing, Bao, Yanqing, Hu, Hai, Liu, Jiameng, Yin, Yi, Ding, Chan, Wang, Shaohui, Yu, Shengqing“…In this study, the T4SS secretion capacities of Brucella rough mutant and its smooth wild-type strain were comparatively investigated, by constructing the firefly luciferase fused T4SS effector, BPE123 and VceC. …”
Publicado 2017
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959por Fiers, Martijn, Hoogenboom, Jorin, Brunazzi, Alice, Wennekes, Tom, Angenent, Gerco C., Immink, Richard G. H.“…In this particular screen, the firefly Luciferase marker was used, fused to the regulatory sequences of the floral meristem identity gene APETALA1 (AP1), which is an early marker for flowering. …”
Publicado 2017
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960“…Cells were transfected with specific miRNA using Lipfectamine 2000. miRNA bingding ability to 3'-UTR region within specific gene was assed by firefly luciferase analysis. Gene and protein expression was eveluated by qRT-PCR and western blot analysis, respectively. …”
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