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181“…Using GSOS with pulse-train modulation, super-resolution and fluorescence lifetime imaging microscopy (FLIM) images can be obtained simultaneously. The super-resolution FLIM capability is experimentally demonstrated with a cell sample on a custom-built two-photon frequency-domain (FD) FLIM system based on radio frequency analog signal processing. …”
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182por Ranjit, Suman, Dvornikov, Alexander, Stakic, Milka, Hong, Suk-Hyun, Levi, Moshe, Evans, Ronald M., Gratton, Enrico“…This is a label free approach towards fast automatable detection of fibrosis in tissue samples. FLIM has previously been used as a method of contrast in different tissues and in this paper phasor approach to FLIM is used to separate collagen I from collagen III, the markers of fibrosis, the largest groups of disorders that are often without any effective therapy. …”
Publicado 2015
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183por Tilden, Elizabeth I., Maduskar, Aditi, Oldenborg, Anna, Sabatini, Bernardo L., Chen, Yao“…Here, we developed a reporter mouse, FL-AK, which expresses FLIM-AKAR in a Cre-dependent manner from the ROSA26 locus. …”
Publicado 2023
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184por Rautaniemi, Kaisa, Vuorimaa-Laukkanen, Elina, Strachan, Clare J., Laaksonen, Timo“…Here, fluorescence-lifetime-imaging microscopy (FLIM) was used to monitor the crystallization of an amorphous pharmaceutical compound, indomethacin. …”
Publicado 2018
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185“…Fluorescence lifetime imaging (FLIM) allows the quantification of sub‐cellular processes in situ, in living cells. …”
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186“…These results suggest that FLIm holds great potential for assessing vascular tissue maturation and functional properties based on tissue autofluorescence.…”
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187“…We surprisingly found that there are on average 45 and 58 FliM molecules in CW and CCW rotating motors, respectively, which are much higher than previous estimates. …”
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188por Yuzhakova, Diana V., Sachkova, Daria A., Shirmanova, Marina V., Mozherov, Artem M., Izosimova, Anna V., Zolotova, Anna S., Yashin, Konstantin S.“…Here, we explored FLIM of NAD(P)H to evaluate the sensitivity of patient-derived glioma cells to temozolomide (TMZ) in vitro. …”
Publicado 2023
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189“…The rotor module consists of a large framework (the C-ring), composed of the FliG, FliM and FliN proteins. FliN and FliM contacts the FliG torque ring to control the direction of flagellar rotation. …”
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190por Alfonso-Garcia, Alba, Cevallos, Stephanie A., Lee, Jee-Yon, Li, Cai, Bec, Julien, Bäumler, Andreas J., Marcu, Laura“…This study demonstrates the ability of intraluminal FLIm to image mucosal lifetime changes in response to inflammatory treatments and supports the development of FLIm as an in vivo imaging technique for monitoring the onset, progression, and treatment of inflammatory diseases.…”
Publicado 2022
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191por Mangion, Sean E., Sandiford, Lydia, Mohammed, Yousuf, Roberts, Michael S., Holmes, Amy M.“…ZnPT particles can be detected in a range of shampoo formulations using both MPM and FLIM, though FLIM is optimal for detection as it allows spectral and lifetime discrimination leading to increased selectivity and sensitivity. …”
Publicado 2022
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192por Borisov, Alexey V., Zakharova, Olga A., Samarinova, Alisa A., Yunusova, Natalia V., Cheremisina, Olga V., Kistenev, Yury V.“…Analysis was performed using a two-photon FLIM device. In total, 165 and 195 FLIM images were recorded for the CP and CCR patient groups, respectively. …”
Publicado 2022
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193“…In addition, the high viscosity of the silicone oil further strengthens the lubricant layer adhesion. Therefore, the FLIM could resist hot liquid and high shear stress (up to 5000 rpm). …”
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194por Bernardi, Mario, Signore, Giovanni, Moscardini, Aldo, Pugliese, Licia Anna, Pesce, Luca, Beltram, Fabio, Cardarelli, Francesco“…Herein, we exploit irinotecan intrinsic fluorescence and fluorescence lifetime imaging microscopy (FLIM) to selectively probe the supramolecular organization of the drug. …”
Publicado 2023
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195“…The simulations were based on a time-gated FLIM instrument, accounting for its instrument response function, gain and noise. …”
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196por Chen, Xi, Kim, Wihan, Serafino, Michael J., Tan, Zheng, Jo, Javier A., Applegate, Brian E.“…Simulation results guided our development of a multimodal OCT/FD-FLIM catheter imaging system for investigating atherosclerosis in coronary arteries.…”
Publicado 2020
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197por Mamontova, Anastasia V., Solovyev, Ilya D., Savitsky, Alexander P., Shakhov, Alexander М., Lukyanov, Konstantin A., Bogdanov, Alexey M.“…Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence decay rate at every pixel of an image. …”
Publicado 2018
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198por Nevin, Austin, Cesaratto, Anna, Bellei, Sara, D'Andrea, Cosimo, Toniolo, Lucia, Valentini, Gianluca, Comelli, Daniela“…Applications of time-resolved photoluminescence spectroscopy (TRPL) and fluorescence lifetime imaging (FLIM) to the analysis of cultural heritage are presented. …”
Publicado 2014
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199por Sorrells, Janet E., Martin, Elisabeth M., Aksamitiene, Edita, Mukherjee, Prabuddha, Alex, Aneesh, Chaney, Eric J., Marjanovic, Marina, Boppart, Stephen A.“…Here, we demonstrate that FLIM can also be used to image and characterize NAD(P)H in single isolated EVs. …”
Publicado 2021
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200por Perinbam, Kumar, Chacko, Jenu V., Kannan, Anerudh, Digman, Michelle A., Siryaporn, Albert“…We established a metabolic profile for P. aeruginosa using fluorescence lifetime imaging microscopy (FLIM), which reports the activity of NADH in live cells. …”
Publicado 2020
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