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441por Golczak, Anna, Prukała, Dorota, Sikorska, Ewa, Gierszewski, Mateusz, Cherkas, Volodymyr, Kwiatek, Dorota, Kubiak, Adam, Varma, Naisargi, Pędziński, Tomasz, Murphree, Shaun, Cibulka, Radek, Mrówczyńska, Lucyna, Kolanowski, Jacek Lukasz, Sikorski, Marek“…Subsequently, fluorescence lifetime imaging experiments (FLIM) were performed on RBC under physiological and oxidative stress conditions alone or in the presence of TMeAll allowing for pinpointing changes caused by those compounds on the intracellular environment of these cells.…”
Publicado 2023
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442por Bu, Wenyu, Lim, Kim Buay, Yu, Yuan Hong, Chou, Ai Mei, Sudhaharan, Thankiah, Ahmed, Sohail“…Forster resonance energy transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) analysis shows that Cdc42, N-WASP and Toca-1 form a trimer complex on the membrane tubules and vesicles and that Cdc42 interaction with N-WASP is critical for complex formation. …”
Publicado 2010
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443por Patalay, Rakesh, Talbot, Clifford, Alexandrov, Yuriy, Munro, Ian, Neil, Mark A. A., König, Karsten, French, Paul M. W., Chu, Anthony, Stamp, Gordon W., Dunsby, Chris“…We explore the diagnostic potential of imaging endogenous fluorophores using two photon microscopy and fluorescence lifetime imaging (FLIM) in human skin with two spectral detection channels. …”
Publicado 2011
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444“…METHODS: The neural imaging process using SPADs can be performed by means of florescence lifetime imaging (FLIM), time correlated single-photon counting (TCSPC), positron emission tomography (PET), and single-photon emission computed tomography (SPECT). …”
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445por Penjweini, Rozhin, Roarke, Branden, Alspaugh, Greg, Gevorgyan, Anahit, Andreoni, Alessio, Pasut, Alessandra, Sackett, Dan L., Knutson, Jay R.“…Herein we present a Förster resonance energy transfer (FRET)-based oxygen sensor, Myoglobin-mCherry, compatible with fluorescence lifetime imaging (FLIM)-based measurement of nicotinamide coenzyme state. …”
Publicado 2020
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446por Rossmann, Florian M., Hug, Isabelle, Sangermani, Matteo, Jenal, Urs, Beeby, Morgan“…This unambiguously confirmed the orientation of the motor switch protein FliM and the binding of a member of the CheY protein family to the outside rim of the C ring. …”
Publicado 2020
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447por Shibata, Akihiro C. E., Ueda, Hiromi H., Eto, Kei, Onda, Maki, Sato, Aiko, Ohba, Tatsuko, Nabekura, Junichi, Murakoshi, Hideji“…By combining paCaMKII with protein activity imaging by 2-photon FLIM-FRET, we demonstrate that paCaMKII activation in clustered spines induces robust sLTP via a mechanism that involves the actin-regulatory small GTPase, Cdc42. …”
Publicado 2021
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448por Law, Ah-Lai, Jalal, Shamsinar, Pallett, Tommy, Mosis, Fuad, Guni, Ahmad, Brayford, Simon, Yolland, Lawrence, Marcotti, Stefania, Levitt, James A., Poland, Simon P., Rowe-Sampson, Maia, Jandke, Anett, Köchl, Robert, Pula, Giordano, Ameer-Beg, Simon M., Stramer, Brian Marc, Krause, Matthias“…Mechanistically, NHSL1 may reduce cell migration efficiency by impeding Arp2/3 activity, as measured in cells using a Arp2/3 FRET-FLIM biosensor, resulting in reduced F-actin density of lamellipodia, and consequently impairing the stability of lamellipodia protrusions.…”
Publicado 2021
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449por Foley, Karl, Altimimi, Haider, Hou, Hailong, Zhang, Yu, McKee, Cody, Papasergi-Scott, Makaía M., Yang, Hongtian, Mayer, Abigail, Ward, Nancy, MacLean, David M., Nairn, Angus C., Stellwagen, David, Xia, Houhui“…Moreover, we demonstrated that I-2 enhanced PP1γ interaction with its major synaptic scaffold, neurabin, by Förster resonance energy transfer (FRET)/Fluorescence lifetime imaging microscopy (FLIM) studies, while having a limited effect on PP1 auto-inhibitory phosphorylation. …”
Publicado 2022
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450por Esteras, Noemí, Blacker, Thomas S., Zherebtsov, Evgeny A., Stelmashuk, Olga A., Zhang, Ying, Wigley, W. Christian, Duchen, Michael R., Dinkova-Kostova, Albena T., Abramov, Andrey Y.“…Employing advanced microscopy imaging of single live cells, including multiphoton fluorescence lifetime imaging microscopy (FLIM) to discriminate between NADH and NADPH, we found that Nrf2 activation increases glucose uptake into neurons and astrocytes. …”
Publicado 2023
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451por Chen, Xiao-Xiao, Gomila, Rosa M., García-Arcos, Juan Manuel, Vonesch, Maxime, Gonzalez-Sanchis, Nerea, Roux, Aurelien, Frontera, Antonio, Sakai, Naomi, Matile, Stefan“…From the functional point of view, the sensitivity of membrane tension imaging in living cells could be doubled, with lifetime differences in FLIM images increasing from 0.55 to 1.11 ns. From a theoretical point of view, the complexity of mechanically coupled chalcogen bonding is explored, revealing, among others, intriguing bifurcated chalcogen bonds.…”
Publicado 2023
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452por Outeiro, Tiago F., Klucken, Jochen, Bercury, Kathryn, Tetzlaff, Julie, Putcha, Preeti, Oliveira, Luis M. A., Quintas, Alexandre, McLean, Pamela J., Hyman, Bradley T.“…METHODOLOGY/PRINCIPAL FINDINGS: Here, we show that α-synuclein adopts a variety of conformations in primary neuronal cultures using fluorescence lifetime imaging microscopy (FLIM). Importantly, we found that dopamine, but not dopamine agonists, induced conformational changes in α-synuclein which could be prevented by blocking dopamine transport into the cell. …”
Publicado 2009
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453por Uemura, Kengo, Farner, Katherine C, Nasser-Ghodsi, Navine, Jones, Phill, Berezovska, Oksana“…RESULTS: By using a Förster resonance energy transfer (FRET)-based technique, fluorescent lifetime imaging microscopy (FLIM), we show that Aβ(42/40 )ratio-modulating factors which target either APP substrate or PS1/γ-secretase affect proximity of the APP-CT to the membrane and change PS1 conformation. …”
Publicado 2011
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454por Berendzen, Kenneth Wayne, Böhmer, Maik, Wallmeroth, Niklas, Peter, Sébastien, Vesić, Marko, Zhou, Ying, Tiesler, Franziska Katharina Elisabeth, Schleifenbaum, Frank, Harter, Klaus“…The candidate interactions were confirmed using in planta BiFC assays and in planta FRET-FLIM assays. From this work, we show that the well characterized protein Calcium Dependent Protein Kinase 3 (CPK3) interacts with APX3, HMGB5, ORP2A and a ricin B-related lectin domain containing protein At2g39050. …”
Publicado 2012
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455por Herbomel, Gaëtan, Hatte, Guillaume, Roul, Julien, Padilla-Parra, Sergi, Tassan, Jean-Pierre, Tramier, Marc“…Using the recently developed E-cadherin FRET tension sensor and a fastFLIM prototype microscope, we were able to measure mechanical forces applied on cadherin at cell-cell junctions. …”
Publicado 2017
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456por García, Carolina, Losada, Alejandro, Sacristán, Miguel A., Martínez-Leal, Juan Fernando, Galmarini, Carlos M., Lillo, M. Pilar“…Here we present an extension of our previous micro-spectroscopy study, that combines Generalized Polarization (GP) images, with the phasor approach and fluorescence lifetime imaging microscopy (FLIM), using a 7-aminocoumarin drug analog (APL(*)) as fluorescence tracer. …”
Publicado 2018
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457por Gaber, Aljaž, Kim, Seung Joong, Kaake, Robyn M., Benčina, Mojca, Krogan, Nevan, Šali, Andrej, Pavšič, Miha, Lenarčič, Brigita“…Second, we also show that EpCAM forms stable dimers on the surface of a cell with pre-formed cell-cell contacts using FLIM-FRET; however, no inter-cellular homo-oligomers were detectable. …”
Publicado 2018
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458por Ahmed, Abdullah R., Owens, Raymond J., Stubbs, Christopher D., Parker, Anthony W., Hitchman, Richard, Yadav, Rahul B., Dumoux, Maud, Hawes, Chris, Botchway, Stanley W.“…Overall, we demonstrate how FRET-FLIM imaging technology can be used to show localisation of S6K1 phosphorylation in living cells and hence a key site of action of inhibitors targeting mTOR phosphorylation.…”
Publicado 2019
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459por Ning, Yingying, Cheng, Shengming, Wang, Jing-Xiang, Liu, Yi-Wei, Feng, Wei, Li, Fuyou, Zhang, Jun-Long“…Time-resolved fluorescence lifetime imaging (FLIM) in the near-infrared region of 900–1700 nm not only allows a deep tissue penetration depth but also offers the unique benefit of the quantitative visualization of molecular events in vivo and is independent of local luminescence intensity and fluorophore concentration. …”
Publicado 2019
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460“…Furthermore, using confocal microscopy, VA-TIRFM, and FRET-FLIM, we provide evidence that stimulation by JA induces phosphorylation- and C-terminus-dependent endocytosis of AtRGS1, which then promotes dissociation of AtRGS1 from AtGPA1. …”
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