“…Here, we present a software package called FLIM eXplorer (FLIMX) for analyzing FLIO data. Specifically, we introduce a new adaptive binning approach as an optimal tradeoff between the spatial resolution and the number of photons required per pixel. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The developed FRET pairs will be useful for designing FRET based sensors and studies employing Fluorescence Lifetime Imaging Microscopy (FLIM).…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Our results showed that in addition to emission spectra, excitation, and fluorescence lifetime imaging microscopy (FLIM) can fruitfully be assessed for optical distinction of PrP deposits associated with distinct prion strains. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These differences in the lipid order were confirmed by fluorescence lifetime imaging (FLIM) at the blue edge of PA emission band. PA probe constitutes thus a new powerful tool for biomembrane research.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We used time-lapse fluorescence microscopy and fluorescence lifetime imaging microscopy (FLIM) to directly monitor the localization and interaction kinetics of a FITC- PuroA peptide on single Candida albicans cells in real time. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we analyze the spatial distribution of Cdc42 activity by moni­toring the dynamics of the Cdc42 FLARE biosensor using the phasor approach to FLIM-FRET. Phasor analysis revealed that Cdc42 is active at all Golgi cisternae and that this activity is controlled by Tuba and ARHGAP10, two Golgi-associated Cdc42 regulators. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Genetic epistasis experiments and FRET-FLIM assays in Drosophila suggest that NS1 interacts directly with the transcriptional mediator, Ci/Gli1. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…This approach is an improvement on existing methods (FLIM, sRET, luxFRET, pFRET), as it does not require single fluorophore standards to be measured with every experiment and the acquisition is intensity independent, allowing the laser power to be optimized for varying levels of fluorophore expression. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we have used intracellular reactive oxygen species (ROS) measurement, and two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of cellular autofluorescent co-enzyme reduced nicotinamide adenine dinucleotide (NADH) to study the inhibitory effects of curcumin and cyclocurcumin in alleviating PD like neurotoxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in neuronal growth factor (NGF) induced differentiated PC12 cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we adapted two-photon excitation microscopy and calibrated time-resolved imaging (FLIM), coupled with patch-clamp electrophysiology, to monitor Ca(2+) concentration ([Ca(2+)]) inside individual astrocytes and principal neurons in acute brain slices. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we report quantitative, real-time, online observations of microscopic viscosity changes in aerosol particles of atmospherically relevant composition, using fluorescence lifetime imaging (FLIM) of viscosity. We show that microviscosity in ozonated oleic acid droplets and secondary organic aerosol (SOA) particles formed by ozonolysis of myrcene increases substantially with decreasing humidity and atmospheric oxidative aging processes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Time-resolved photoluminescence (PL) and fluorescence lifetime imaging microscopy (FLIM) studies of the thick-shell QD monolayer reveal narrower lifetime distribution in comparison with the thin-shell QD monolayer. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we show that protein interacting with C terminal kinase 1 (PICK1), a protein known to bind to the GluA2 subunit of AMPARs and associated with AMPAR trafficking, was pulled-down from brain homogenates and synaptosomes when using Arc as immobilized bait. Fluctuation and FLIM-FRET-Phasor analysis revealed direct interaction between these proteins when co-expressed that was increased under depolarizing conditions in live cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We used time-gated fluorescence lifetime imaging microscopy (FLIM) with a G4 fluorescent probe, 3,6-bis(1-methyl-2-vinylpyridinium) carbazole diiodide (o-BMVC), to measure the number of o-BMVC foci, which may represent G4 foci, in cells as a common signature to distinguish cancer cells from normal cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We demonstrate the utility of FLiP by monitoring the lifetime of FLIM-AKAR, a genetically encoded fluorescent reporter of PKA phosphorylation, in populations of direct and indirect pathway striatal projection neurons in mice receiving food rewards. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Herein, we compare a range of fluorophores, assess their usability to study RLK interactions by FRET‐based fluorescence lifetime imaging (FLIM) and explore their differences in FRET efficiency. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Intracellular melanin-mapped FLIM phasors showed a linear distribution indicating that HCM melanins are a ratio of two fluorophores, eumelanin and pheomelanin. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Fluorescence lifetime imaging microscopy (FLIM) of NADH was used to corroborate the changes in bioenergetics. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Visualization of the stained living cells was performed both by fluorescence microscopy imaging and by fluorescence lifetime imaging under two photon excitation (TPE-FLIM), confirming their cellular uptake and the capability of staining the cell nucleus.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Intracellular fate of GCD@DOX, tracked by FLIM, Raman mapping and fluorescence microscopy, evidenced the efficient cellular uptake of GCD@DOX and the presence of DOX in the nucleus, without graphene carrier. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso