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41“…CONCLUSIONS: The FLIM-FRET analyzer software is a flexible application for lifetime-based FRET analysis. …”
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42por Sherrard, Alice, Bishop, Paul, Panagi, Melanie, Villagomez, Maria Beatriz, Alibhai, Dominic, Kaidi, Abderrahmane“…Collectively, we present an adaptable chromatin FLIM method for examining chromatin structure and establish its utility in mammalian cells.…”
Publicado 2018
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43por Bhatt, Vineet, Mohapatra, Anwesha, Anand, Swadha, Kuntal, Bhusan K., Mande, Sharmila S.“…In this communication, we present a novel annotation resource to accurately identify pathway presence by using gene context. Our tool FLIM-MAP (Functionally Important Modules in bacterial Metabolic Pathways) predicts biologically relevant functional units called ‘GCMs’ (Gene Context based Modules) from a given metabolic reaction network. …”
Publicado 2018
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44“…Time-correlated single-photon counting (TCSPC) and fluorescence lifetime imaging microscopy (FLIM) experiments show the presence of neutral and protonated CLZ (1.3–3.8 ns) and weakly interacting aggregates (0.4–0.9 ns), along with H- and J-type aggregates (<0.1 ns). …”
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46por Rodimova, Svetlana, Elagin, Vadim, Karabut, Maria, Koryakina, Irina, Timin, Alexander, Zagainov, Vladimir, Zyuzin, Mikhail, Zagaynova, Elena, Kuznetsova, Daria“…A fundamentally new label-free approach for studying liver pathology is fluorescence-lifetime imaging microscopy (FLIM). We obtained FLIM data on both the free and bound forms of NAD(P)H, which is associated with different metabolic pathways. …”
Publicado 2021
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48por Tittelmeier, Jessica, Druffel-Augustin, Silke, Alik, Ania, Melki, Ronald, Nussbaum-Krammer, Carmen“…Here we use fluorescence lifetime imaging microscopy (FLIM) to investigate seeded aggregation of α-Syn in a biosensor cell line. …”
Publicado 2022
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49por Izosimova, Anna V., Shirmanova, Marina V., Shcheslavskiy, Vladislav I., Sachkova, Daria A., Mozherov, Artem M., Sharonov, George V., Zagaynova, Elena V., Yuzhakova, Diana V.“…Fluorescence lifetime imaging (FLIM) of metabolic coenzymes is a promising innovative technology for the assessment of the functional status of cells. …”
Publicado 2022
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50por Venditti, Rossella, Rega, Laura Rita, Masone, Maria Chiara, Santoro, Michele, Polishchuk, Elena, Sarnataro, Daniela, Paladino, Simona, D’Auria, Sabato, Varriale, Antonio, Olkkonen, Vesa M., Di Tullio, Giuseppe, Polishchuk, Roman, De Matteis, Maria AntoniettaEnlace del recurso
Publicado 2019
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52“…We present a new approach that employs fluorescence lifetime imaging microscopy (FLIM) to detect Förster resonance energy transfer (FRET) when using the β-lactamase (BlaM) assay. …”
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53por Kashirina, Alena S., López-Duarte, Ismael, Kubánková, Markéta, Gulin, Alexander A., Dudenkova, Varvara V., Rodimova, Svetlana A., Torgomyan, Hayk G., Zagaynova, Elena V., Meleshina, Aleksandra V., Kuimova, Marina K.Enlace del recurso
Publicado 2020
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54por Sagar, Md Abdul Kader, Cheng, Kevin P., Ouellette, Jonathan N., Williams, Justin C., Watters, Jyoti J., Eliceiri, Kevin W.“…NAD(P)H lifetime-based discrimination techniques have previously been used to develop metabolic cell signatures for diverse cell types including immune cells such as macrophages. However, FLIM could be even more effective in characterizing cell types if machine learning was used to classify cells by utilizing FLIM parameters for classification. …”
Publicado 2020
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55por Shaik, Tanveer Ahmed, Alfonso-Garcia, Alba, Richter, Martin, Korinth, Florian, Krafft, Christoph, Marcu, Laura, Popp, Jürgen“…In conclusion, FLIm parameters and Raman spectroscopy were used to monitor the degree of cross-linking non-destructively.…”
Publicado 2020
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57por Sarfraz, Nadia, Moscoso, Emilia, Oertel, Therese, Lee, Harrison J., Ranjit, Suman, Braselmann, Esther“…Here, we present the fluorescence lifetime-based platform Riboglow-FLIM. Our system requires a smaller tag and has superior cell contrast when compared with intensity-based detection. …”
Publicado 2023
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58por Coucke, Quinten, Parveen, Nagma, Fernández, Guillermo Solís, Qian, Chen, Hofkens, Johan, Debyser, Zeger, Hendrix, Jelle“…Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. …”
Publicado 2023
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59por Vitali, Marco, Picazo, Fernando, Prokazov, Yury, Duci, Alessandro, Turbin, Evgeny, Götze, Christian, Llopis, Juan, Hartig, Roland, Visser, Antonie J. W. G., Zuschratter, Werner“…We propose a Wide-Field Multi-Parameter FLIM method (WFMP-FLIM) aimed to monitor continuously living cells under minimum light intensity at a given illumination energy dose. …”
Publicado 2011
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60“…Calcium concentration was monitored using the calcium indicator Green 1, 2-photon excitation, and fluorescence lifetime imaging microscopy (FLIM). Data were analyzed using the phasor representation. …”
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