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Mostrando 161 - 180 Resultados de 641 Para Buscar '"Flims"', tiempo de consulta: 0.36s Limitar resultados
  1. 161
    por Vu, Cong Quang, Arai, Satoshi
    Publicado 2023
    “…Specifically, we focus on two types of go-FLIM, namely Förster resonance energy transfer (FRET)–FLIM and single-fluorescent protein (FP)-based FLIM biosensors, and discuss their unique characteristics and benefits. …”
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    Online Artículo Texto
    Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors
  2. 162
    “…However, NAD(P)H FLIM has not been established as a metabolic proxy in bacteria. …”
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    Online Artículo Texto
    Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy
  3. 163
    “…Approach: Frequency-domain FLIM was integrated into a surgical microscope, which enabled parallel wide-field white-light and fluorescence imaging. …”
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    Surgical microscope with integrated fluorescence lifetime imaging for 5-aminolevulinic acid fluorescence-guided neurosurgery
  4. 164
    “…To achieve accurate discrimination between metabolically diverse cellular subpopulations, appropriate approaches to FLIM data collection and analysis are needed. In this paper, the unique capability of FLIM to attain the overarching goal of discriminating metabolic heterogeneity is demonstrated. …”
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    Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity
  5. 165
    “…Furthermore, 2p-FLIM allows distinguishing free and bound populations of NADH by their different fluorescence lifetimes within single infected cells. …”
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    Online Artículo Texto
    Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues
  6. 166
    “…A 15-patient testing group was used to compare FLIM results with traditional histopathology diagnosis. …”
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    Rapid diagnosis and intraoperative margin assessment of human lung cancer with fluorescence lifetime imaging microscopy
  7. 167
    “…This is complemented by a walk-through of the data analysis for this specific FLIM FRET data set.…”
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    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
  8. 168
    “…The ability to safely acquire co-registered FLIm-IVUS data in vivo using Dextran40 solution flushing was demonstrated in swine coronary arteries. …”
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    In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system
  9. 169
    “…Multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) is extensively proposed as a non-invasive optical method to study tissue metabolism. …”
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    Contribution of autofluorescence from intracellular proteins in multiphoton fluorescence lifetime imaging
  10. 170
    “…Endoscopic-FLIM (e-FLIM) data was validated by comparison with data acquired via conventional FLIM and e-FLIM was found to be accurate for both bright bead and dim live cell samples. …”
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    Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells
  11. 171
    “…These results show that FLIm can detect the loss of GAG in articular cartilage and support further investigation into the feasibility of in vivo FLIm arthroscopy.…”
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    Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging
  12. 172
    “…High-quality FLIm OCT image of a human coronary artery specimen was acquired. …”
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    Dual-modality fluorescence lifetime imaging-optical coherence tomography intravascular catheter system with freeform catheter optics
  13. 173
    “…Switching of flagellar motor rotation sense dictates bacterial chemotaxis. Multi-subunit FliM-FliG rotor rings couple signal protein binding in FliM with reversal of a distant FliG C-terminal (FliG(C)) helix involved in stator contacts. …”
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    The Gearbox of the Bacterial Flagellar Motor Switch
  14. 174
    “…In Escherichia coli, the binding of the chemotaxis signaling protein CheY to the flagellar motor protein FliM induces reversal of the motor rotation. Using molecular dynamics (MD) simulations and parallel cascade selection MD (PaCS-MD), we show that high pressure increases the water density in the first hydration shell of CheY and considerably induces water penetration into the CheY-FliM interface. …”
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    High pressure inhibits signaling protein binding to the flagellar motor and bacterial chemotaxis through enhanced hydration
  15. 175
    “…Switching of bacterial flagellar rotation is caused by large domain movements of the FliG protein triggered by binding of the signal protein CheY to FliM. FliG and FliM form adjacent multi-subunit arrays within the basal body C-ring. …”
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    Coevolved Mutations Reveal Distinct Architectures for Two Core Proteins in the Bacterial Flagellar Motor
  16. 176
    “…Clinical widefield maFLIM dermoscopy imaging of 41 benign and 19 malignant pigmented skin lesions from 30 patients were acquired prior to tissue biopsy sampling. …”
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    Discrimination of cancerous from benign pigmented skin lesions based on multispectral autofluorescence lifetime imaging dermoscopy and machine learning
  17. 177
    “…While NAD(P)H fluorescence lifetime imaging (FLIM) can detect changes in flux through the TCA cycle and electron transport chain (ETC), it remains unclear whether NAD(P)H FLIM is sensitive to other potential fates of glucose. …”
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    Protein-bound NAD(P)H Lifetime is Sensitive to Multiple Fates of Glucose Carbon
  18. 178
    “…Both subsystems share the same excitation/illumination optical path and are simultaneously raster scanned on the sample to generate coregistered OCT volumes and FLIM images. The developed OCT/FLIM system was capable of a maximum A-line rate of 59 KHz for OCT and a pixel rate of up to 30 KHz for FLIM. …”
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    A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization
  19. 179
    “…We verified the performance of the approach by applying NAD(P)H-FLIM on a stromal-like cell line and identified a different group of enzymes that were active in the cell nuclei as compared to the cytoplasm. …”
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    Systematic Enzyme Mapping of Cellular Metabolism by Phasor-Analyzed Label-Free NAD(P)H Fluorescence Lifetime Imaging
  20. 180
    “…Fluorescence lifetime imaging (FLIM) can therefore provide a high content analysis (HCA) modality to map protein-protein interactions (PPIs) with applications in drug discovery, systems biology and basic research. …”
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    Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation
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