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Mostrando 1 - 20 Resultados de 641 Para Buscar '"Flims"', tiempo de consulta: 0.33s Limitar resultados
  1. 1
    “…Background: Fluorescence lifetime imaging (FLIm) is a spectroscopic imaging technique able to characterize the composition of luminal surface of arterial vessels. …”
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    Investigating Origins of FLIm Contrast in Atherosclerotic Lesions Using Combined FLIm-Raman Spectroscopy
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    “…We compare Pseudo-FLIM to FLIM bi-exponential and phasor analyses of synthetic melanin, melanocytes/keratinocytes coculture and in vivo human skin. …”
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    In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses
  3. 3
    “…CONCLUSION: The combined advantages suggest that the TagGFP-TagRFP is one of the most efficient green/red couples available to date for FRET/FLIM analyses to monitor interaction of proteins of interest in living cells and to generate FRET-based sensors for various applications. …”
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    Practical and reliable FRET/FLIM pair of fluorescent proteins
  4. 4
    “…The nanosensors were satisfactorily applied to the detection of glucose into MDA-MB-231 cells with FLIM. The long PL lifetimes of the QD nanoparticles made them easily discernible from cell autofluorescence, thereby improving selectivity in their sensing applications. …”
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    A Quantum Dot-Based FLIM Glucose Nanosensor
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    “…We demonstrated that multiphoton FLIM allows the discrimination between native DHI, DHICA, Dopa eumelanins, pheomelanin, and mixed eu-/pheo-melanin polymers. …”
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    Multiphoton FLIM Analyses of Native and UVA-Modified Synthetic Melanins
  6. 6
    “…In this study, we use bulk fluorescence lifetime measurements and fluorescence lifetime imaging microscopy (FLIM) to examine the stability of retinol in phosphatidylcholine (PC) multilamellar and unilamellar vesicles with and without cholesterol. …”
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    Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM
  7. 7
    “…Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. …”
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    FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring
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    “…Fluorescence Lifetime Imaging (FLIM) is an intrinsically quantitative method to screen for protein–protein interactions and is frequently used to record the outcome of signal transduction events. …”
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    Dynamic FRET-FLIM based screening of signal transduction pathways
  9. 9
    “…For this purpose, we developed a lightweight (4.8 kg) portable instrument for macroscopic fluorescence lifetime imaging (FLIM). It is based on a time-correlated single-photon avalanche diode (SPAD) camera to acquire the FLIM images and a pulsed 440 nm diode laser to excite the varnish fluorescence. …”
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    Visualising varnish removal for conservation of paintings by fluorescence lifetime imaging (FLIM)
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    “…We present a quantitative Förster resonance energy transfer (FRET)–based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. …”
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    Quantitative analysis of chromatin compaction in living cells using FLIM–FRET
  11. 11
    “…In this paper, we present the application of NMF to multispectral time-domain FLIM data to obtain a new set of FLIM features (relative abundance of constituent fluorophores). …”
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    Application of non-negative matrix factorization to multispectral FLIM data analysis
  12. 12
    por Oliveira, Ana F., Yasuda, Ryohei
    Publicado 2013
    “…Previously, fluorescence lifetime imaging (FLIM) of fluorescent resonance energy transfer (FRET)-based Ras activity sensors, FRas and FRas-F, have been demonstrated to be useful for measuring the spatiotemporal dynamics of Ras signaling in subcellular micro-compartments. …”
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    An Improved Ras Sensor for Highly Sensitive and Quantitative FRET-FLIM Imaging
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    “…Here, using genetic and pharmacological approaches to perturb NAD(P)H metabolism, we find that fluorescence lifetime imaging (FLIM) differentiates quantitatively between the two cofactors. …”
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    Separating NADH and NADPH fluorescence in live cells and tissues using FLIM
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    “…This study uses FLIM to measure pharmacologically induced metabolic changes that occur during the browning of white fat. …”
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    Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM
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    “…The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Förster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. …”
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    Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
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    “…Significance: The potential of fluorescence lifetime imaging microscopy (FLIM) is recently being recognized, especially in biological studies. …”
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    FLIM data analysis based on Laguerre polynomial decomposition and machine-learning
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    Environmental and Geographical (EG) Image Classification Using FLIM and CNN Algorithms
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    “…FLIM (Fluorescence Lifetime Imaging Microscopy) is a powerful tool that could be used in the future to diagnose islet cell recovery after therapy. …”
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    Contrast-Free FLIM Reveals Metabolic Changes in Pathological Islets of Langerhans
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    Retracted: Environmental and Geographical (EG) Image Classification Using FLIM and CNN Algorithms
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    “…Here, we establish our RNA tagging and imaging platform Riboglow-FLIM for complex cellular imaging applications by systematically evaluating FLIM capabilities. …”
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    Establishing Riboglow-FLIM to visualize noncoding RNAs inside live zebrafish embryos
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