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5901por Vazquez, Neftali, Sanchez, Lilia, Marks, Rebecca, Martinez, Eduardo, Fanniel, Victor, Lopez, Alma, Salinas, Andrea, Flores, Itzel, Hirschmann, Jesse, Gilkerson, Robert, Schuenzel, Erin, Dearth, Robert, Halaby, Reginald, Innis-Whitehouse, Wendy, Keniry, Megan“…We describe custom donor vector construction using Gibson assembly with the commonly utilized pcDNA3 vector as the backbone. …”
Publicado 2018
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5902“…The application of the Gibson–Ashby model allowed for a comparison of the mechanical performance of the lattice materials with that of human bone. …”
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5903por Siddall, Alex, Williams, Abbie Ann, Sanders, Jason, Denton, Jai A., Madden, Dean, Schollar, John, Bryk, Jarosław“…It is built on a high-copy plasmid backbone and is easily extensible thanks to a common primer set that facilitates Gibson assembly of PCR-generated or synthesized DNA parts into the target vector. …”
Publicado 2023
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5904por Benedetti, Matteo, Klarin, Johanna, Johansson, Frida, Fontanari, Vigilio, Luchin, Valerio, Zappini, Gianluca, Molinari, Alberto“…The stiffness and yield strength were correlated to relative density according to the Gibson and Ashby model. For a constant porosity, the stiffness and the yield strength varied between two extremes represented by the cubic structure (stretching-dominated deformation) and the cross structure (bending-dominated deformation). …”
Publicado 2019
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5905“…The SmartBac system integrates the univector plasmid-fusion system, Gibson assembly method and polyprotein strategy to construct the final transfer plasmid. …”
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5906“…If an open approach is required, the modified Gibson approach can be used for lateral hip access, whereas the Smith-Petersen approach provides anterior hip access. …”
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5907“…ApE can be used in designing plasmids and other constructs via in silico simulation of cloning methods such as PCR, Gibson assembly, restriction-ligation assembly and Golden Gate assembly. …”
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5908por Barber, Colin“…J. Crews, Mr W. P. R. Gibson, Professor G. F. A. Harding, Dr D. A. Jeffreys, Dr D. …”
Publicado 1980
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5909“…PYS is usually restricted to lattice materials that are stretching dominated according to the Gibson–Ashby model. Contrary to this long-held assumption, this work shows that PYS can also occur in various bending-dominated Ti-6Al-4V lattices with increasing relative density. …”
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5910por Hornibrook, Jeremy, Kalin, Catherine, Lin, Emily, O'Beirne, Greg A., Gourley, John“…This paper evaluated the diagnostic power of electrocochleography (ECochG) in detecting Ménière's disease (MD) as compared with two subjective assessment methods, including the clinical guidelines provided by the American Academy of Otolaryngology—Head and Neck Surgery Committee on Hearing Equilibrium and the Gibson score. A retrospective study of 250 suspected MD cases was conducted. …”
Publicado 2012
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5911por Zhao, Hui, Chen, Chuansheng, Zhang, Hongchuan, Zhou, Xinlin, Mei, Leilei, Chen, Chunhui, Chen, Lan, Cao, Zhongyu, Dong, Qi“…One group was asked to learn the associations between magnitude (dot patterns) and the meaningless Gibson symbols, and the other group learned the associations between spatial order (horizontal positions on the screen) and the same set of symbols. …”
Publicado 2012
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5912por Juhas, Mario, Evans, Lewis D. B., Frost, Joe, Davenport, Peter W., Yarkoni, Orr, Fraser, Gillian M., Ajioka, James W.“…We combined the Red recombinase system of bacteriophage λ and Isothermal Gibson Assembly for rapid integration of novel DNA constructs into the E. coli chromosome. …”
Publicado 2014
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5913“…Proffitt's embodied approach to perception is deeply indebted to Gibson's ecological approach to visual perception, in particular the idea that the primary objects of perception are affordances or what the environment offers for action. …”
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5914“…As an alternative strategy we have developed and optimized a new protocol for assembling the cHA by exploiting Gibson Assembly. • This method also requires precise primer design, but it is rapid and methodologically simple to perform. …”
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5915“…By generating a conditional null allele, one study proposed that the dpp stripe is critical for patterning but not for growth (Akiyama and Gibson, 2015). In contrast, using a membrane-anchored nanobody to trap Dpp, the other study proposed that Dpp dispersal from the stripe is required for patterning and also for medial wing disc growth, at least in the posterior compartment (Harmansa et al., 2015). …”
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5916“…The proposed software then finds the most cost-effective combination of synthetic and PCR-prepared repository fragments to build the plasmid via Gibson assembly(®). It finds existing DNA sequences in both user-specified and public DNA databases: iGEM, Addgene, and DNASU. …”
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5917“…One method is based on a modified Gibson assembly, while the other relies on a direct assembly and integration of linear PCR products by yeast homologous recombination. …”
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5918por Fathirezaie, Zahra, Abbaspour, Kosar, Badicu, Georgian, Zamani Sani, Seyed Hojjat, Nobari, Hadi“…These results were discussed and interpreted based on the types of environmental affordances, Gibson’s theory, Bronfenbrenner theory, and child-friendly environment. …”
Publicado 2021
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5919por Cheng, Lingyin, Zhou, Xiaoqing, Zheng, Yuling, Tang, Chengcheng, Liu, Yu, Zheng, Shuwen, Liu, Yang, Zhou, Jizeng, Li, Chuan, Chen, Min, Lai, Liangxue, Zou, Qingjian“…TALE trimers with nonrepetitive DNA sequences were amplified by PCR and sequentially assembled via Gibson assembly. Our method is cost-effective, requires only commonly used basic molecular biology reagents, and takes only 2 h from target sequence analysis to completion. …”
Publicado 2021
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5920“…Furthermore, the incorporation of a relationship between Young’s modulus and the coefficient of pore volume variation to the Gibson–Ashby model is proposed.…”
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