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  1. 5901
    “…We describe custom donor vector construction using Gibson assembly with the commonly utilized pcDNA3 vector as the backbone. …”
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    A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone
  2. 5902
    “…The application of the Gibson–Ashby model allowed for a comparison of the mechanical performance of the lattice materials with that of human bone. …”
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    Titanium Lattice Structures Produced via Additive Manufacturing for a Bone Scaffold: A Review
  3. 5903
    “…It is built on a high-copy plasmid backbone and is easily extensible thanks to a common primer set that facilitates Gibson assembly of PCR-generated or synthesized DNA parts into the target vector. …”
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    Unigems: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
  4. 5904
    “…The stiffness and yield strength were correlated to relative density according to the Gibson and Ashby model. For a constant porosity, the stiffness and the yield strength varied between two extremes represented by the cubic structure (stretching-dominated deformation) and the cross structure (bending-dominated deformation). …”
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    Study of the Compression Behaviour of Ti6Al4V Trabecular Structures Produced by Additive Laser Manufacturing
  5. 5905
    “…The SmartBac system integrates the univector plasmid-fusion system, Gibson assembly method and polyprotein strategy to construct the final transfer plasmid. …”
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    SmartBac, a new baculovirus system for large protein complex production
  6. 5906
    “…If an open approach is required, the modified Gibson approach can be used for lateral hip access, whereas the Smith-Petersen approach provides anterior hip access. …”
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    Excision of Heterotopic Ossification around the Hip: Arthroscopic and Open Techniques
  7. 5907
    por Davis, M. Wayne, Jorgensen, Erik M.
    Publicado 2022
    “…ApE can be used in designing plasmids and other constructs via in silico simulation of cloning methods such as PCR, Gibson assembly, restriction-ligation assembly and Golden Gate assembly. …”
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    ApE, A Plasmid Editor: A Freely Available DNA Manipulation and Visualization Program
  8. 5908
    por Barber, Colin
    Publicado 1980
    “…J. Crews, Mr W. P. R. Gibson, Professor G. F. A. Harding, Dr D. A. Jeffreys, Dr D. …”
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    International Evoked Potentials Symposium
  9. 5909
    por Zhong, H Z, Li, C W, Das, R, Gu, J F, Qian, M
    Publicado 2023
    “…PYS is usually restricted to lattice materials that are stretching dominated according to the Gibson–Ashby model. Contrary to this long-held assumption, this work shows that PYS can also occur in various bending-dominated Ti-6Al-4V lattices with increasing relative density. …”
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    Post-yield softening of bending-dominated metal metamaterials
  10. 5910
    “…This paper evaluated the diagnostic power of electrocochleography (ECochG) in detecting Ménière's disease (MD) as compared with two subjective assessment methods, including the clinical guidelines provided by the American Academy of Otolaryngology—Head and Neck Surgery Committee on Hearing Equilibrium and the Gibson score. A retrospective study of 250 suspected MD cases was conducted. …”
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    Transtympanic Electrocochleography for the Diagnosis of Ménière's Disease
  11. 5911
    “…One group was asked to learn the associations between magnitude (dot patterns) and the meaningless Gibson symbols, and the other group learned the associations between spatial order (horizontal positions on the screen) and the same set of symbols. …”
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    Is Order the Defining Feature of Magnitude Representation? An ERP Study on Learning Numerical Magnitude and Spatial Order of Artificial Symbols
  12. 5912
    “…We combined the Red recombinase system of bacteriophage λ and Isothermal Gibson Assembly for rapid integration of novel DNA constructs into the E. coli chromosome. …”
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    Escherichia coli Flagellar Genes as Target Sites for Integration and Expression of Genetic Circuits
  13. 5913
    “…Proffitt's embodied approach to perception is deeply indebted to Gibson's ecological approach to visual perception, in particular the idea that the primary objects of perception are affordances or what the environment offers for action. …”
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    Embodied Perception: A Proposal to Reconcile Affordance and Spatial Perception
  14. 5914
    por Ferrara, Francesca, Temperton, Nigel
    Publicado 2016
    “…As an alternative strategy we have developed and optimized a new protocol for assembling the cHA by exploiting Gibson Assembly. • This method also requires precise primer design, but it is rapid and methodologically simple to perform. …”
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    Chimeric influenza haemagglutinins: Generation and use in pseudotype neutralization assays
  15. 5915
    por Matsuda, Shinya, Affolter, Markus
    Publicado 2017
    “…By generating a conditional null allele, one study proposed that the dpp stripe is critical for patterning but not for growth (Akiyama and Gibson, 2015). In contrast, using a membrane-anchored nanobody to trap Dpp, the other study proposed that Dpp dispersal from the stripe is required for patterning and also for medial wing disc growth, at least in the posterior compartment (Harmansa et al., 2015). …”
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    Dpp from the anterior stripe of cells is crucial for the growth of the Drosophila wing disc
  16. 5916
    por Timmons, Joshua J., Densmore, Doug
    Publicado 2020
    “…The proposed software then finds the most cost-effective combination of synthetic and PCR-prepared repository fragments to build the plasmid via Gibson assembly(®). It finds existing DNA sequences in both user-specified and public DNA databases: iGEM, Addgene, and DNASU. …”
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    Repository-based plasmid design
  17. 5917
    “…One method is based on a modified Gibson assembly, while the other relies on a direct assembly and integration of linear PCR products by yeast homologous recombination. …”
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    Genomic integration of unclonable gene expression cassettes in Saccharomyces cerevisiae using rapid cloning‐free workflows
  18. 5918
    “…These results were discussed and interpreted based on the types of environmental affordances, Gibson’s theory, Bronfenbrenner theory, and child-friendly environment. …”
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    The Effect of Environmental Contexts on Motor Proficiency and Social Maturity of Children: An Ecological Perspective
  19. 5919
    “…TALE trimers with nonrepetitive DNA sequences were amplified by PCR and sequentially assembled via Gibson assembly. Our method is cost-effective, requires only commonly used basic molecular biology reagents, and takes only 2 h from target sequence analysis to completion. …”
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    Simple and Rapid Assembly of TALE Modules Based on the Degeneracy of the Codons and Trimer Repeats
  20. 5920
    por Ćwieka, Karol, Skibiński, Jakub
    Publicado 2022
    “…Furthermore, the incorporation of a relationship between Young’s modulus and the coefficient of pore volume variation to the Gibson–Ashby model is proposed.…”
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    Elastic Properties of Open Cell Metallic Foams—Modeling of Pore Size Variation Effect
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