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44“…Here, we describe a novel method to prepare DNA molecules containing defined arrays of the ‘601’ nucleosome positioning sequence by exploiting Gibson Assembly cloning. The approaches presented here provide a more accessible and efficient means to generate arrays of nucleosome positioning motifs, and facilitate a high degree of control over the linker sequences between these motifs. …”
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45por De Mauro, Domenico, Rovere, Giuseppe, Smakaj, Amarildo, Marino, Silvia, Ciolli, Gianluca, Perna, Andrea, Battiato, Concetto, El Ezzo, Omar, Liuzza, Francesco“…BACKGROUND: The purpose of the study is to evaluate clinical and radiological outcomes in those patients with femoral head fracture, treated with open reduction and internal fixation through Gibson approach and Ganz flip trochanter osteotomy. …”
Publicado 2021
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46“…Here, we present a faster protocol for the simultaneous plasmid restriction digestion and Gibson assembly of vectors for the synthesis of both riboprobes and double-stranded RNAs for in situ and RNA interference experiments, respectively. …”
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49por Moors, Pieter, Verfaillie, Karl, Daems, Thalia, Pomianowska, Iwona, Germeys, Filip“…Throughout the years, the results of two of these (Gibson and Pick, 1963; Cline, 1967) have been interpreted differently by a number of authors. …”
Publicado 2016
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50por Li, Chenhao, Ji, Chonghui, Huguet‐Tapia, José C., White, Frank F., Dong, Hansong, Yang, Bing“…Here, we present an easy and efficient cloning technique to clone TALe genes selectively through in vitro homologous recombination and single‐strand annealing, and demonstrate the feasibility of this approach with four different Xoo strains. Based on the Gibson assembly strategy, two complementary vectors with scaffolds that can preferentially capture all TALe genes from a pool of genomic fragments were designed. …”
Publicado 2019
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51por Ni, Na, Deng, Fang, He, Fang, Wang, Hao, Shi, Deyao, Liao, Junyi, Zou, Yulong, Wang, Hongwei, Zhao, Piao, Hu, Xue, Chen, Connie, Hu, Daniel A., Sabharwal, Maya, Qin, Kevin H., Wagstaff, William, Qin, David, Hendren-Santiago, Bryce, Haydon, Rex C., Luu, Hue H., Reid, Russell R., Shen, Le, He, Tong-Chuan, Fan, Jiaming“…Moreover, the Ad-making techniques have not been improved for the past two decades. Gibson DNA Assembly (GDA) technology allows one-step isothermal DNA assembly of multiple overlapping fragments. …”
Publicado 2021
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52“…Under the management of an enhanced recovery after surgery (ERAS) protocol, a combined and modified Gibson and ilioinguinal (MGMII) approach was used to avoid unnecessary soft tissue trauma during tumor resection and therefore advantageous to patients’ return to normal life. …”
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53por Allen, Jeffrey, Flanagan, Michael, Pathak, Sunita, Emenecker, Samantha, Emenecker, Ryan, Strader, Lucia“…Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. …”
Publicado 2023
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55por Pan, Haibin, Yan, Yuqian, Zhang, Jing, Zhao, Shan, Feng, Liqiang, Ou, Junxian, Cao, Na, Li, Min, Zhao, Wei, Wan, Chengsong, Ismail, Ashrafali M., Rajaiya, Jaya, Chodosh, James, Zhang, Qiwei“…The pBRAdV14 plasmid, generated by Gibson Assembly, was analyzed and verified by PCR, restriction enzymes digestion and the sequencing. …”
Publicado 2018
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56por Shen, Chun-Yang, Xue, Wei, Pang, Chong, Alireza, Asem, Mao, Xiaonan, Han, Jiahui, Chen, Haonan, Fu, Chunzheng“…In this study, we sequenced and characterized the complete mitochondrial genome (mitogenome) of Quasilineus sinicus Gibson, 1990 (Heteronemertea, Nemertea) using Illumina sequencing technology. …”
Publicado 2022
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