“…The cloning efficiency was similar to that of the commercial In-Fusion method employing a proprietary DNA polymerase, but higher than that of the Gibson method utilizing T5 exonuclease, Phusion DNA polymerase, and DNA ligase. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We can assemble up to seven such barcoded fragments into one plasmid by using one of the existing DNA assembly methods, including CLIVA, Gibson assembly, In-fusion cloning, and restriction enzyme-based methods. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The studies were interpreted and evaluated against the back-ground of two descriptions of the driving task: Gibson and Crooks’ description of driving as the visually guided selection of a driving path through the unobstructed field of safe travel; and Endsley’s situation awareness model, highlighting the influence of drivers’ interpreta-tions and mental capacities (e.g., cognitive load, memory capacity, etc.) for successful task performance. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In particular, the strut morphology, the fracture characterization, the metallographic structure, and the X-ray phase identification were analyzed. Additionally, the Gibson-Ashby prediction model was adapted for each lattice topology, indicating the theoretical compressive strength and Young modulus. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The vector was constructed in a single step process using Gibson Assembly, which facilitated an easy and seamless assembly of multiple inserts. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Genetic constructs were Gibson assembled and electrotransformed into V. cholerae. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We employ self-circularization with and without splints, a Gibson cloning-based approach, and novel 2 novel methods for generating pseudocircular DNA. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…High‐efficiency transformation has enabled vector‐free mutagenesis for which we have employed circular minimalistic constructs (knockout minicircles) containing only allelic exchange essentials that were generated by Gibson assembly. Preparation of competent cells using sucrose and electroporation/integration of minicircles had virtually no detectable off‐target promutagenic effect. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The marine ribbon worm genus Tetranemertes Chernyshev, 1992 currently includes three species: the type species T.antonina (Quatrefages, 1846) from the Mediterranean Sea, T.rubrolineata (Kirsteuer, 1965) from Madagascar, and T.hermaphroditica (Gibson, 1982) from Australia. Seven new species are described: T.bifrostsp. nov., T.ocelatasp. nov., T.majinbuuisp. nov., and T.pastafariensissp. nov. from the Caribbean Sea (Panamá), and three species, T.unistriatasp. nov., T.paulayisp. nov., and T.arabicasp. nov., from the Indo-West Pacific (Japan and Oman). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The infectious full-length cDNA clone was constructed using Gibson assembly and subsequent infection of Nicotiana benthamiana plants by Rhizobium radiobacter inoculation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…CONCLUSIONS: We made a low Gibson incision for a route for the hand-assisted procedure as well as a window for open surgery in dissecting the distal ureter and extracting the surgical specimens. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…It feeds into the work of continental psychologists in the early 20th century, influencing important figures such as Merleau-Ponty, Piaget, and Gibson. But it has not yet been taken up by mainstream cognitive neuroscience and psychology. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…There is a wealth of evidence showing that increasing the distance between an argument and its head leads to more processing effort, namely, locality effects; these are usually associated with constraints in working memory (DLT: Gibson, 2000; activation-based model: Lewis and Vasishth, 2005). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: We have developed pydna, an extensible, free and open source Python library for simulating basic molecular biology DNA unit operations such as restriction digestion, ligation, PCR, primer design, Gibson assembly and homologous recombination. A cloning strategy expressed as a pydna script provides a description that is complete, unambiguous and stable. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The concept of affordance, originally suggested by Gibson (1979) has been incorporated in a recent model of interactive behavior that draws from findings in non-human primates, namely the “affordance competition hypothesis” (Cisek, 2007). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Finally, novel plasmid-free strategies were used for transformation of REG19 based on Gibson assembly.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We demonstrate the capability of cloning influenza-specific TCRs within 10 days using single-cell polymerase chain reaction (PCR) and Gibson Assembly techniques. This process can be accelerated to 5 days by generating receptor libraries, requiring only the exchange of the antigen-specific CDR3 region into an existing backbone. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Transformation via a natural competence protocol could use DNA PCR products amplified directly from Gibson Assembly mixture for efficient genetic modification.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Second, the estimated PSF is fitted to Gibson's parametric PSF model via optimization, and depth-variant PSFs are generated. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso