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6041“…The cloning efficiency was similar to that of the commercial In-Fusion method employing a proprietary DNA polymerase, but higher than that of the Gibson method utilizing T5 exonuclease, Phusion DNA polymerase, and DNA ligase. …”
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6042por Ma, Xiaoqiang, Liang, Hong, Cui, Xiaoyi, Liu, Yurou, Lu, Hongyuan, Ning, Wenbo, Poon, Nga Yu, Ho, Benjamin, Zhou, Kang“…We can assemble up to seven such barcoded fragments into one plasmid by using one of the existing DNA assembly methods, including CLIVA, Gibson assembly, In-fusion cloning, and restriction enzyme-based methods. …”
Publicado 2019
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6043“…The studies were interpreted and evaluated against the back-ground of two descriptions of the driving task: Gibson and Crooks’ description of driving as the visually guided selection of a driving path through the unobstructed field of safe travel; and Endsley’s situation awareness model, highlighting the influence of drivers’ interpreta-tions and mental capacities (e.g., cognitive load, memory capacity, etc.) for successful task performance. …”
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6044por Cosma, Cosmin, Drstvensek, Igor, Berce, Petru, Prunean, Simon, Legutko, Stanisław, Popa, Catalin, Balc, Nicolae“…In particular, the strut morphology, the fracture characterization, the metallographic structure, and the X-ray phase identification were analyzed. Additionally, the Gibson-Ashby prediction model was adapted for each lattice topology, indicating the theoretical compressive strength and Young modulus. …”
Publicado 2020
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6045“…The vector was constructed in a single step process using Gibson Assembly, which facilitated an easy and seamless assembly of multiple inserts. …”
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6046por Karpov, Dmitry S., Goncharenko, Anna V., Usachev, Evgenii V., Vasina, Daria V., Divisenko, Elizaveta V., Chalenko, Yaroslava M., Pochtovyi, Andrei A., Ovchinnikov, Roman S., Makarov, Valentin V., Yudin, Sergei M., Tkachuk, Artem P., Gushchin, Vladimir A.“…Genetic constructs were Gibson assembled and electrotransformed into V. cholerae. …”
Publicado 2021
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6047Publicado 2003Tabla de Contenidos: “…Hunter -- Local surveillance systems / Catherine Quigley, James J. Gibson, and Paul R. Hunter -- Developing an integrated system of health and technical data / Alan Thompson -- National surveillance systems / Rosalind Stanwell-Smith, Yvonne Andersson, and Deborah A. …”
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6048Circularization of rv0678 for Genotypic Bedaquiline Resistance Testing of Mycobacterium tuberculosis“…We employ self-circularization with and without splints, a Gibson cloning-based approach, and novel 2 novel methods for generating pseudocircular DNA. …”
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6049“…High‐efficiency transformation has enabled vector‐free mutagenesis for which we have employed circular minimalistic constructs (knockout minicircles) containing only allelic exchange essentials that were generated by Gibson assembly. Preparation of competent cells using sucrose and electroporation/integration of minicircles had virtually no detectable off‐target promutagenic effect. …”
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6050por Cherneva, Irina, Ellison, Christina I., Zattara, Eduardo E., Norenburg, Jon L., Schwartz, Megan L., Junoy, Juan, Maslakova, Svetlana A.“…The marine ribbon worm genus Tetranemertes Chernyshev, 1992 currently includes three species: the type species T.antonina (Quatrefages, 1846) from the Mediterranean Sea, T.rubrolineata (Kirsteuer, 1965) from Madagascar, and T.hermaphroditica (Gibson, 1982) from Australia. Seven new species are described: T.bifrostsp. nov., T.ocelatasp. nov., T.majinbuuisp. nov., and T.pastafariensissp. nov. from the Caribbean Sea (Panamá), and three species, T.unistriatasp. nov., T.paulayisp. nov., and T.arabicasp. nov., from the Indo-West Pacific (Japan and Oman). …”
Publicado 2023
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6051“…The infectious full-length cDNA clone was constructed using Gibson assembly and subsequent infection of Nicotiana benthamiana plants by Rhizobium radiobacter inoculation. …”
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6052“…CONCLUSIONS: We made a low Gibson incision for a route for the hand-assisted procedure as well as a window for open surgery in dissecting the distal ureter and extracting the surgical specimens. …”
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6053“…It feeds into the work of continental psychologists in the early 20th century, influencing important figures such as Merleau-Ponty, Piaget, and Gibson. But it has not yet been taken up by mainstream cognitive neuroscience and psychology. …”
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6054por Nicenboim, Bruno, Vasishth, Shravan, Gattei, Carolina, Sigman, Mariano, Kliegl, Reinhold“…There is a wealth of evidence showing that increasing the distance between an argument and its head leads to more processing effort, namely, locality effects; these are usually associated with constraints in working memory (DLT: Gibson, 2000; activation-based model: Lewis and Vasishth, 2005). …”
Publicado 2015
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6055por Pereira, Filipa, Azevedo, Flávio, Carvalho, Ângela, Ribeiro, Gabriela F, Budde, Mark W, Johansson, Björn“…RESULTS: We have developed pydna, an extensible, free and open source Python library for simulating basic molecular biology DNA unit operations such as restriction digestion, ligation, PCR, primer design, Gibson assembly and homologous recombination. A cloning strategy expressed as a pydna script provides a description that is complete, unambiguous and stable. …”
Publicado 2015
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6056“…The concept of affordance, originally suggested by Gibson (1979) has been incorporated in a recent model of interactive behavior that draws from findings in non-human primates, namely the “affordance competition hypothesis” (Cisek, 2007). …”
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6057“…Finally, novel plasmid-free strategies were used for transformation of REG19 based on Gibson assembly.…”
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6058por Guo, Xi-zhi J, Dash, Pradyot, Calverley, Matthew, Tomchuck, Suzanne, Dallas, Mari H, Thomas, Paul G“…We demonstrate the capability of cloning influenza-specific TCRs within 10 days using single-cell polymerase chain reaction (PCR) and Gibson Assembly techniques. This process can be accelerated to 5 days by generating receptor libraries, requiring only the exchange of the antigen-specific CDR3 region into an existing backbone. …”
Publicado 2016
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6059“…Transformation via a natural competence protocol could use DNA PCR products amplified directly from Gibson Assembly mixture for efficient genetic modification.…”
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6060“…Second, the estimated PSF is fitted to Gibson's parametric PSF model via optimization, and depth-variant PSFs are generated. …”
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