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2201por Eckel, Florian, Küsters, Franziska, Drossel, Bernhard, Konert, Markus, Mattes, Hans, Schopf, Stefan“…The loop-mediated isothermal amplification (LAMP) method has been utilized for the detection of isolated virus-specific RNA. …”
Publicado 2020
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2202“…In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. …”
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2203“…Upon the amplification of the target gene by an Eriochrome Black T-mediated LAMP reaction, the color of the LAMP reaction was changed from violet to blue that was real-time recorded by a smartphone camera. …”
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2204por Ganguli, Anurup, Mostafa, Ariana, Berger, Jacob, Aydin, Mehmet Y., Sun, Fu, de Ramirez, Sarah A. Stewart, Valera, Enrique, Cunningham, Brian T., King, William P., Bashir, Rashid“…As assay protocols and primer sequences become widely known, many laboratories perform diagnostic tests using methods such as RT-PCR or reverse transcription loop mediated isothermal amplification (RT-LAMP). Here, we report an RT-LAMP isothermal assay for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and demonstrate the assay on clinical samples using a simple and accessible point-of-care (POC) instrument. …”
Publicado 2020
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2205por Zhu, Yuan-Shou, Shao, Ning, Chen, Jian-Wei, Qi, Wen-Bao, Li, Yang, Liu, Peng, Chen, Yan-Jing, Bian, Su-Ying, Zhang, Yan, Tao, Sheng-Ce“…LAMP primers targeting five ASFV genes (B646L, B962L, C717R, D1133L, and G1340L) were designed and pre-fixed in Hive-Chip. …”
Publicado 2020
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2206por Ding, Xiong, Yin, Kun, Li, Ziyue, Pandian, Vikram, Smyth, Joan A., Helal, Zeinab, Liu, Changchun“…Also, this CHB-enhanced LAMP (CHB-LAMP) assay was successfully demonstrated to detect B. burgdorferi DNA extracted from tick species, showing comparable results to real-time PCR assay. …”
Publicado 2020
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2207por Hardinge, Patrick, Baxani, Divesh K., McCloy, Thomas, Murray, James A. H., Castell, Oliver K.“…Stable, uniform LAMP–BART droplets are generated with low cost equipment. …”
Publicado 2020
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2208por Ji, Chao, Xue, Shuxia, Yu, Min, Liu, Jinyu, Zhang, Qin, Zuo, Feng, Zheng, Qiuyue, Zhao, Liangjuan, Zhang, Hongwei, Cao, Jijuan, Wang, Ke, Liu, Wei, Zheng, Wenjie“…Herein, we provide a novel, rapid, and simple approach, named dual reverse transcriptional colorimetric loop-mediated isothermal amplification (dRT-cLAMP) assay, to accelerate the detection of the SARS-CoV-2 virus without using expensive equipment. …”
Publicado 2021
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2209“…METHODS: Autophagic pathways were examined in the context of the heterogeneity intrinsic to gastric/GEJ adenocarcinoma, utilizing immunohistochemistry targeting specific proteins within the pathways (p62, LAMP2A, LC3B). We examined whole sections of normal and dysplastic gastric mucosa, as well as a tissue microarray of adenocarcinomas. …”
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2210por Chen, Ying-Yi, Sun, Yi-Chen, Tsai, Chia-Ying, Chu, Hsiao-Sang, Wu, Jo-Hsuan, Chang, Huai-Wen, Chen, Wei-Li“…In presumed-healthy areas of the diseased eyes diagnosed by slit-lamp biomicroscopy, SD-OCT detected abnormal changes in the POV in 100% of the eyes in both groups. …”
Publicado 2021
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2211por Rauchegger, Teresa, Osl, Antonia, Nowosielski, Yvonne, Angermann, Reinhard, Palme, Christoph, Haas, Gertrud, Steger, Bernhard“…The major problems were fogging of the lenses (87.6%) or slit lamp oculars (69.9%), reduced operability of the slit lamp due to protective barriers (60.2%) and time delay due to disinfection measures (68.1%). …”
Publicado 2021
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2212por Osabutey, Angelina F., Seo, Bo Yoon, Kim, A-Young, Ha, Thu Anh Thi, Jung, JinKyo, Goergen, Georg, Owusu, Ebenezer Oduro, Lee, Gwan-Seok, Koh, Young Ho“…The Sf00067 6 primer LAMP (Sf6p-LAMP) assay established in this study was able to diagnose various genotype-based strains of FAW captured in Korea and FAWs collected from Benin, Africa. …”
Publicado 2022
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2213por Shen, Jie-Ni, Ye, Jing-Yi, Lao, Meng-Xiao, Wang, Chu-Qiao, Wu, Dong-Hong, Chen, Xiao-Ying, Lin, Li-Hong, Geng, Wen-Yan, Guo, Xu-Guang“…In our study, a real-time fluorescence loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of UU. …”
Publicado 2022
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2214por Dewhurst, Robert E., Heinrich, Tatjana, Watt, Paul, Ostergaard, Paul, Marimon, Jose M., Moreira, Mariana, Houldsworth, Philip E., Rudrum, Jack D., Wood, David, Kõks, Sulev“…Independent validation of our saliva-based RT-LAMP technology on an automated LAMP instrument coined the “Sentinel”, found 98.7% sensitivity, 97.6% specificity, and 98% accuracy against a RT-PCR comparator assay, confirming its suitability for surveillance screening. …”
Publicado 2022
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2215por Cawley, Niamh X., Lyons, Anna T., Abebe, Daniel, Luke, Rachel, Yerger, Julia, Telese, Rebecca, Wassif, Christopher A., Bailey-Wilson, Joan E., Porter, Forbes D.“…Previously, we characterized the appearance of a complex asparagine-linked glycosylated form of lysosome-associated membrane protein 1 (LAMP1) in the cerebellum of Npc1(−/−) mice. This LAMP1 form was found on activated microglia, and its appearance correlated both spatially and temporally with cerebellar Purkinje neuron loss. …”
Publicado 2022
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2216por Li, Xiaoju, Yu, Qianqian, Zhao, Runze, Guo, Xinyan, Liu, Chenlin, Zhang, Kuo, Zhang, Wangqian, Liu, Jinghan, Yu, Jinzheng, Wang, Shuning, Hao, Qiang, Li, Weina, Zhang, Wei, Li, Meng, Zhang, Yingqi, Zhang, Cun, Gao, Yuan“…Then, exosomes with high multi-siRNA loading and HCC-specific targeting were established by fusing the SP94 peptide and the N-terminal RNA recognition motif (RRM) of U1-A with the exosomal membrane protein Lamp2b. The results from the in vitro and in vivo experiments indicate that this tumor-targeting nano-delivery system (Exo(SP94-lamp2b-RRM)-multi-siRNA) could enhance sorafenib-induced ferroptosis and overcome sorafenib resistance. …”
Publicado 2022
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2217por Qin, Chao, Liu, Jiajia, Zhu, Wenqi, Zeng, Muchu, Xu, Ke, Ding, Jinmei, Zhou, Hao, Zhu, Jianshen, Ke, Yuqing, Li, Lai Yan, Sheng, Gaoyuan, Li, Zhuoru, Luo, Huaixi, Jiang, Shengyao, Chen, Kangchun, Ding, Xianting, Meng, He“…Both CRISPR-RPA and CRISPR-LAMP systems permit highly rapid, sensitive, specific, and low-cost Cas12a-mediated visual diagnostic of ASFV for point-of-care testing (POCT) applications.…”
Publicado 2022
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2218por Turathum, Bongkoch, Gao, Er-Meng, Grataitong, Khwanthana, Liu, Yu-Bing, Wang, Ling, Dai, Xue, Chian, Ri-Cheng“…Gene expression involved in ceramide synthesis (CERS1, SPTL1, and SMPD1) and autophagy (BECN1, LAMP, and PC3) were significantly higher in CCs with endometriosis according to FASN, BECN1, and LAMP protein expressions. …”
Publicado 2022
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2219por Moser, Nicolas, Yu, Ling-Shan, Rodriguez Manzano, Jesus, Malpartida-Cardenas, Kenny, Au, Anselm, Arkell, Paul, Cicatiello, Chiara, Moniri, Ahmad, Miglietta, Luca, Wang, Wen-Hung, Wang, Sheng Fan, Holmes, Alison, Chen, Yen-Hsu, Georgiou, Pantelis“…The assay on our LoC platform (RT-eLAMP) was shown to match performance on a gold-standard fluorescence-based real-time instrument (RT-qLAMP) with synthetic DENV-1 and DENV-2 RNA and extracted RNA from 9 DENV-2 clinical isolates, achieving quantitative detection in under 15 min. …”
Publicado 2022
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2220“…Loop-mediated isothermal amplification (LAMP) coupled with a reverse transcription step (RT-LAMP) is an alternative diagnostic method that can easily overcome these obstacles, when coupled with CRISPR/Cas it can eliminate false positives. …”
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