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461por Arjuna, Srividya, Venkataram, Rajesh, Dechamma, Pandyanda Nanjappa, Chakraborty, Gunimala, Babu, Nishith, D’Cruz, Audrey, Hosmane, Giridhar Belur, Chakraborty, Anirban“…In this study, we used cell free DNA as a template in AS-LAMP assay (CF-LAMP) for non-invasive detection of two hotspot EGFR mutations (T790M, and L858R) and compared its efficiency with ultrasensitive droplet digital PCR (ddPCR) assay. …”
Publicado 2020
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462por Wang, Rui, Qian, Chunyan, Pang, Yanan, Li, Miaomiao, Yang, Yu, Ma, Haijing, Zhao, Manying, Qian, Feng, Yu, Hang, Liu, Zhenping, Ni, Ting, Zheng, Yan, Wang, Yongming“…Here, we propose a one-pot visual SARS-CoV-2 detection system named “opvCRISPR” by integrating reverse transcription loop-mediated isothermal amplification (RT-LAMP) and Cas12a cleavage in a single reaction system. …”
Publicado 2021
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463“…This paper reports the development of a LAMP (Loop-Mediated Isothermal Amplification) specific for Cff, that allows the detection of Cff in infected seeds, both by fluorescence and visual monitoring, after 30 min of reaction and with a detection limit at around 4 fg/μL of pure Cff genomic DNA.…”
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465por Prasannakumar, M. K., Parivallal, P. Buela, Pramesh, Devanna, Mahesh, H. B., Raj, Edwin“…Further, the sensitivity of the LAMP, HDA, PCR, and qPCR assays were compared wherein; the LAMP assay was highly sensitive up to 100 fg of template DNA. …”
Publicado 2021
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466“…In a hydroponic culture, lateral root length and N uptake were decreased in both overexpression and knockdown of TaLAMP1 at the seedling stage. In the field experiment with normal N supply, the grain yield of overexpression of TaLAMP1-3B is significantly reduced (14.5%), and the knockdown of TaLAMP1 was significantly reduced (15.5%). …”
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467por Jang, Seong Hyun, Han, Young Joon, Lee, Sang Yoon, Lee, Geonho, Jung, Jae Woong, Cho, Kwan Hyun, Choi, Jun“…Based on the characteristics of the peeled-off polyimide films, the Xe Flash lamp based lift-off mechanism was identified as photothermal decomposition. …”
Publicado 2021
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469por Minami, Kenta, Masutani, Ryota, Suzuki, Youichi, Kubota, Meri, Osaka, Naofumi, Nakanishi, Toyofumi, Nakano, Takashi, Ukimura, Akira“…MATERIALS AND METHODS: RNA was extracted by using the QIAamp Viral RNA Mini Kit. The RT-LAMP assay was performed by using the Loopamp® 2019-SARS-CoV-2 detection reagent kit and 10-fold serial dilutions of known viral load RT-LAMP were used to measure Tt, which is the time until the turbidity exceeds the threshold. …”
Publicado 2021
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470por Rizzo, Domenico, Moricca, Salvatore, Bracalini, Matteo, Benigno, Alessandra, Bernardo, Umberto, Luchi, Nicola, Da Lio, Daniele, Nugnes, Francesco, Cappellini, Giovanni, Salemi, Chiara, Cacciola, Santa Olga, Panzavolta, Tiziana“…Here we report the development of a highly efficient, low-cost, and rapid method for detecting the beetle, or even just its biological traces, from environmental samples: the loop-mediated isothermal amplification (LAMP) assay. The method, designed on the 28S ribosomal RNA gene, showed high specificity and sensitivity, with no cross reactivity to other bark beetles and wood-boring insects. …”
Publicado 2021
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471por Zhang, Mimi, Liu, Jinfeng, Shen, Zhiqiang, Liu, Yongxin, Song, Yang, Liang, Yu, Li, Zhende, Nie, Lingmei, Fang, Yanjun, Zhao, Youquan“…RESULTS: We embed paper impregnated with LAMP reagent and specific primers in multiple reaction chambers of the microchip. …”
Publicado 2021
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473por Amoah, Isaac Dennis, Mthethwa, Nonsikelelo Precios, Pillay, Leanne, Deepnarain, Nashia, Pillay, Kriveshin, Awolusi, Oluyemi Olatunji, Kumari, Sheena, Bux, Faizal“…Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has the potential to become a cheaper and faster option for monitoring COVID-19 infections through wastewater-based epidemiology. …”
Publicado 2021
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474por Selvam, Kasturi, Najib, Mohamad Ahmad, Khalid, Muhammad Fazli, Mohamad, Suharni, Palaz, Fahreddin, Ozsoz, Mehmet, Aziah, Ismail“…In this work, we review and summarize reverse-transcription loop-mediated isothermal amplification (RT-LAMP) CRISPR-based diagnostic techniques for detecting SARS-CoV-2.…”
Publicado 2021
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475por Loo, Jessica, Woodward, Maria A., Prajna, Venkatesh, Kriegel, Matthias F., Pawar, Mercy, Khan, Mariam, Niziol, Leslie M., Farsiu, Sina“…PURPOSE: To assess clinical applicability of automatic image analysis in microbial keratitis (MK) by evaluating the relationship between biomarker measurements on slit-lamp photography (SLP) and best-corrected visual acuity (BCVA). …”
Publicado 2021
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476por Bat-Ochir, Chinbayar, Kim, Yeon-Sook, Kim, Han Gyeul, Lee, Si Seok, Lee, Han Woo, Park, Hee Kyung“…Dual-labeled PNA probe used RT-LAMP molecular rapid assay targeting SARS-CoV-2 ORF1ab and N genes was developed, and the analytical, clinical performances for detection of SARS-CoV-2 RNA extracted from clinical nasopharyngeal swab specimens were evaluated in this study. …”
Publicado 2021
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477“…While the majority of the proposed ideas for reducing the energy cost of the street lighting system are based on light emitting diode lamps, they are not suitable for high-pressure sodium lamps, which continue to dominate in developing countries. …”
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478por Koyama, Ayumi, Miyazaki, Dai, Nakagawa, Yuji, Ayatsuka, Yuji, Miyake, Hitomi, Ehara, Fumie, Sasaki, Shin-ichi, Shimizu, Yumiko, Inoue, Yoshitsugu“…Corneal opacities are important causes of blindness, and their major etiology is infectious keratitis. Slit-lamp examinations are commonly used to determine the causative pathogen; however, their diagnostic accuracy is low even for experienced ophthalmologists. …”
Publicado 2021
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479por Salazar, Andrea, Ochoa-Corona, Francisco M., Olson, Jennifer D., Babu, Binoy, Paret, Mathews“…The limit of detection (LoD) was 1pg/μL and 1 fg/μL using Bst 2.0 LAMP and GspSSD LD quantitative LAMP, respectively. …”
Publicado 2021
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480por Pu, Ruiyang, Liu, Sha, Ren, Xiaoyu, Shi, Dian, Ba, Yupei, Huo, Yanbei, Zhang, Wenling, Ma, Lingling, Liu, Yanyan, Yang, Yan, Cheng, Ning“…The sensitivity and FNR were also significantly affected by the reference method. Comparing RT-LAMP with established suboptimal RT-PCR may exaggerate the performance of RT-LAMP. …”
Publicado 2022
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