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561por Chen, Chuang, Lu, Jia, Long, Bo, Rao, Zhengyuan, Gao, Yuan, Wang, Weina, Gao, Wenfeng, Yang, Jun, Zhang, Shu“…In this study, we combined loop-mediated isothermal amplification (LAMP) with lateral flow biosensors (LFB) to detect M. kansasii, by targeting the species-specific sequence of rpoB, a gene which encodes the β subunit of bacterial RNA polymerase. …”
Publicado 2020
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562por Gibson, Kristen M., Kain, Renate, Luqmani, Raashid A., Ross, Colin J., Cabral, David A., Brown, Kelly L.“…LAMP-2-ANCA titers did not correlate with measures of disease activity (pVAS, CRP, or ESR) at the time of diagnosis. …”
Publicado 2021
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563por Bokelmann, Lukas, Nickel, Olaf, Maricic, Tomislav, Pääbo, Svante, Meyer, Matthias, Borte, Stephan, Riesenberg, Stephan“…In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.…”
Publicado 2021
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565“…OBJECTIVE: We evaluated the CE-approved eazyplex® SARS-CoV-2, a ready-to-use real time RT-LAMP assay for identification of the SARS-CoV-2 N and ORF8 genes from swabs in less than 30 min without RNA extraction. …”
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566“…OBJECTIVE: In this study, the performance of a commercially available malaria LAMP assay (Alethia® Malaria Plus LAMP) was evaluated using retrospective clinical samples obtained from travelers returning to the United States of America (USA). …”
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567Colorimetric RT-LAMP SARS-CoV-2 diagnostic sensitivity relies on color interpretation and viral loadpor Aoki, Mateus Nóbrega, de Oliveira Coelho, Bruna, Góes, Luiz Gustavo Bentim, Minoprio, Paola, Durigon, Edison Luiz, Morello, Luis Gustavo, Marchini, Fabricio Klerynton, Riediger, Irina Natassja, do Carmo Debur, Maria, Nakaya, Helder I., Blanes, Lucas“…The use of RT-LAMP (reverse transcriptase—loop mediated isothermal amplification) has been considered as a promising point-of-care method to diagnose COVID-19. …”
Publicado 2021
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568por Nguyen, Quang Thach, Ngo, Minh Dung, Truong, Thanh Hung, Nguyen, Duy Chinh, Nguyen, Minh Chau“…New lamp prototypes were tested in 7 field experiments in three different provinces in Vietnam. …”
Publicado 2021
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569“…DNA was extracted from washes, tested by LAMP and melt curve analysis, and time to positive (TTP) values compared. …”
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570por Köck, Johannes, Gottschalk, Christoph, Ulrich, Sebastian, Schwaiger, Karin, Gareis, Manfred, Niessen, Ludwig“…To selectively identify macrocyclic trichothecene producing S. chartarum isolates, a set of sat14 gene-specific primers was designed and applied in a loop-mediated isothermal amplification (LAMP) assay using neutral red for visual signal detection. …”
Publicado 2021
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571por Kobayashi, Gerson Shigeru, Brito, Luciano Abreu, Moreira, Danielle de Paula, Suzuki, Angela May, Hsia, Gabriella Shih Ping, Pimentel, Lylyan Fragoso, de Paiva, Ana Paula Barreto, Dias, Carolina Regoli, Lourenço, Naila Cristina Vilaça, Oliveira, Beatriz Araujo, Manuli, Erika Regina, Corral, Marcelo Andreetta, Cavaçana, Natale, Mitne-Neto, Miguel, Sales, Maria Mirtes, Dell’ Aquila, Luiz Phellipe, Filho, Alvaro Razuk, Parrillo, Eduardo Fagundes, Mendes-Corrêa, Maria Cássia, Sabino, Ester Cerdeira, Costa, Silvia Figueiredo, Leal, Fabio Eudes, Sgro, Germán Gustavo, Farah, Chuck Shaker, Zatz, Mayana, Passos-Bueno, Maria Rita“…We aimed to develop a direct RT-LAMP (reverse transcription loop-mediated isothermal amplification) workflow for viral detection in saliva, and to provide more information regarding its potential in curbing COVID-19 transmission. …”
Publicado 2021
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572por Ng, David, Pinharanda, Ana, Vogt, Merly C., Litwin-Kumar, Ashok, Stearns, Kyle, Thopte, Urvashi, Cannavo, Enrico, Enikolopov, Armen, Fiederling, Felix, Kosmidis, Stylianos, Noro, Barbara, Rodrigues-Vaz, Ines, Shayya, Hani, Andolfatto, Peter, Peterka, Darcy S., Tabachnik, Tanya, D’Armiento, Jeanine, Goldklang, Monica, Bendesky, Andres“…Here, we describe WHotLAMP, a rapid molecular test to detect SARS-CoV-2 in saliva. …”
Publicado 2021
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573por Daddy Gaoh, Soumana, Kweon, Ohgew, Lee, Yong-Jin, LiPuma, John J., Hussong, David, Marasa, Bernard, Ahn, Youngbeom“…Comparison of LAMP with a qPCR assay using 1440 test sets showed higher sensitivity: 60.6% in nuclease-free water and 42.4% in CHX solution with LAMP vs. 51.3% and 31.1%, respectively, with qPCR. …”
Publicado 2021
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574por Tang, Zifan, Nouri, Reza, Dong, Ming, Yang, Jianbo, Greene, Wallace, Zhu, Yusheng, Yon, Michele, Nair, Meera Surendran, Kuchipudi, Suresh V., Guan, Weihua“…Here, we present an RT-LAMP coupled glass nanopore digital counting method for rapid detection of SARS-CoV-2. …”
Publicado 2022
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575por Avila, Héctor Gabriel, Risso, Marikena Guadalupe, Cabrera, Marta, Ruybal, Paula, Repetto, Silvia Analía, Butti, Marcos Javier, Trangoni, Marcos David, Santillán, Graciela, Pérez, Verónica Mirtha, Periago, María Victoria“…Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. …”
Publicado 2021
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576“…Danon disease (DD) is a rare glycogen storage lysosomal disorder caused by mutations in the LAMP2 gene. Patients with DD are usually characterized clinically by severe multisystem syndromes. …”
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577por Padzil, Faiz, Mariatulqabtiah, Abdul Razak, Tan, Wen Siang, Ho, Kok Lian, Isa, Nurulfiza Mat, Lau, Han Yih, Abu, Jalila, Chuang, Kuo-Pin“…This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses.…”
Publicado 2021
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578“…OBJECTIVE: This study outlines how we used mobile apps and semantic web standards such as HTTP 2.0, Representational State Transfer, JSON (JavaScript Object Notation), JSON Schema, Transport Layer Security (version 1.3), Advanced Encryption Standard-256, OpenAPI, HTML5, and Vega, in conjunction with patient and provider feedback to completely update a previous version of mindLAMP. METHODS: The Learn, Assess, Manage, and Prevent (LAMP) platform addresses the abovementioned challenges in enhancing clinical insight by supporting research, data analysis, and implementation efforts around PGHD as an open-source solution with freely accessible and shared code. …”
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579por Ta-Tang, Thuy-Huong, Berzosa, Pedro, Rubio, José Miguel, Romay-Barja, María, Ncogo, Policarpo, Agudo, Diego, Herrador, Zaida, Cerrada-Gálvez, Laura, Benito, Agustín“…MAIN CONCLUSIONS: Loa-LAMP is an appropriate method for loiasis diagnosis in endemic areas. …”
Publicado 2022
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580por Cook, Bradley W. M., Kobasa, Kaitlyn, Tamayo, Marielou, Theriault, Natasha, Gordon Pappas, Diane, Theriault, Steven S.“…Employees and their household contacts were encouraged to self-collect saliva samples that were pooled for routine testing using an established colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. In brief, individual or a maximum of four saliva samples were pooled and heat-inactivated to render microorganisms, especially SARS-CoV-2, non-infectious prior to being added to RT-LAMP assay tubes containing either the human sample control gene, RNase P, or a region of the SARS-CoV-2 gene, ORF1ab. …”
Publicado 2022
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