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761por Ding, Sheng, Chen, Rong, Chen, Gangyi, Li, Mei, Wang, Jiayu, Zou, Jiawei, Du, Feng, Dong, Juan, Cui, Xin, Huang, Xin, Deng, Yun, Tang, Zhuo“…We reported a novel SNP typing method based on the probe-enhanced loop-mediated isothermal amplification (PE-LAMP), which combines the oligonucleotide probe with a conventional LAMP to realize the SNP discrimination by analyzing the great discrepancy in amplification efficiency. …”
Publicado 2019
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762por Wang, Deguo, Wang, Yongzhen, Zhu, Kai, Shi, Lijia, Zhang, Meng, Yu, Jianghan, Liu, Yanhong“…Five specific Loop-mediated Isothermal Amplification (LAMP) primers targeting the internal transcribed spacer (ITS) of cassava were designed, genomic DNA was extracted, the LAMP reaction system was optimized, and the specificity of the primers was verified with genomic DNA of cassava, Ipomoea batatas, Zea mays, and Solanum tuberosum; the detection limit was determined with a serial dilution of adulterated sweet potato starch with cassava starch, and the real-time LAMP method for the detection of the cassava-derived ingredient in sweet potato noodles was established. …”
Publicado 2019
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763por Wang, Xiaoping, Li, Chun, Wang, Qiyan, Li, Weili, Guo, Dongqing, Zhang, Xuefeng, Shao, Mingyan, Chen, Xu, Ma, Lin, Zhang, Qian, Wang, Wei, Wang, Yong“…Further experiments demonstrated that these effects were mediated through upregulation of Beclin1 and LAMP1. The mTOR agonist MHY1485 was shown to abrogate the effect of TSA via the UNC-51-like kinase 1 (ULK1)-Beclin1/TFEB-LAMP1 signaling pathway in vitro, demonstrating that TSA protects against DIC by promoting autophagy via the Beclin1/LAMP1 signaling pathway. …”
Publicado 2019
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764por King, Kevin M., Hawkins, Nichola J., Atkins, Sarah, Dyer, Paul S., West, Jonathan S., Fraaije, Bart A.“…BACKGROUND: Loop‐mediated isothermal amplification (LAMP) assays, which operate at a single temperature and require no postreaction processing, have been described for rapid species‐specific detection of numerous fungi. …”
Publicado 2019
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765por Stella, Stefania, Gottardi, Enrico Marco, Favout, Valeria, Barragan Gonzalez, Eva, Errichiello, Santa, Vitale, Silvia Rita, Fava, Carmen, Luciano, Luigia, Stagno, Fabio, Grimaldi, Francesco, Pironi, Lucrezia, Sargas Simarro, Claudia, Vigneri, Paolo, Izzo, Barbara“…Specifically, the p190- and p210-positive samples were amplified by Q-LAMP with a median threshold time (Tt) of 26.70 min (range: 24.45–31.80 min) and 20.26 min (range: 15.25-34.57 min), respectively. …”
Publicado 2019
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766por Jiang, Kuiyu, Zhu, Ying, Liu, Wenxin, Feng, Yufei, He, Lili, Guan, Weikun, Hu, Wenxia, Shi, Dongfang“…The objective of this study was to establish a loop-mediated isothermal amplification (LAMP) method for the detection of F5 fimbriae gene in Enterotoxigenic Escherichia coli. …”
Publicado 2012
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767por Dillard, Kati J., Ochs, Matthias, Niskanen, Julia E., Arumilli, Meharji, Donner, Jonas, Kyöstilä, Kaisa, Hytönen, Marjo K., Anttila, Marjukka, Lohi, Hannes“…The substitution resides in the LAMP domain adjacent to a conserved disulfide bond. …”
Publicado 2020
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768“…In this research, the efficiency of Salmonella detection was compared using the following four methods: conventional loop-mediated isothermal amplification (LAMP), PCR, quantitative LAMP (qLAMP), and qPCR. …”
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769por ITOH, Megumi, HIRANO, Yuki, YAMAKAWA, Kazuhiro, YASUTOMI, Ichiro, KURAMOTO, Keiko, FURUOKA, Miyuki, YAMADA, Kazutaka“…LAMP was examined using DNA extracts obtained by four methods. …”
Publicado 2020
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770por Ali, Zahir, Aman, Rashid, Mahas, Ahmed, Rao, Gundra Sivakrishna, Tehseen, Muhammad, Marsic, Tin, Salunke, Rahul, Subudhi, Amit K., Hala, Sharif M., Hamdan, Samir M., Pain, Arnab, Alofi, Fadwa S., Alsomali, Afrah, Hashem, Anwar M., Khogeer, Asim, Almontashiri, Naif A.M., Abedalthagafi, Malak, Hassan, Norhan, Mahfouz, Magdy M.“…Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. …”
Publicado 2020
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771por Sandoval, Helga P, Lane, Stephen, Slade, Stephen, Donnenfeld, Eric D, Potvin, Richard, Solomon, Kerry D“…Orientation was measured at the microscope on the day of surgery, and with the slit lamp at all other visits. Day 1, 1-month and 3-month images of the lens orientation were captured with a slit lamp camera. …”
Publicado 2020
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772por Gurka, Jiri, Piherova, Lenka, Majer, Filip, Chaloupka, Anna, Zakova, Daniela, Pelak, Ondrej, Krebsova, Alice, Peichl, Petr, Krejci, Jan, Freiberger, Tomas, Melenovsky, Vojtech, Kautzner, Josef, Kalina, Tomas, Sikora, Jakub, Kubanek, Milos“…AIMS: Danon disease (DD) is a rare X‐linked disorder caused by mutations in the lysosomal‐associated membrane protein type 2 gene (LAMP2). DD is difficult to distinguish from other causes of dilated or hypertrophic cardiomyopathy (HCM) in female patients. …”
Publicado 2020
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773por Amambo, Glory Ngongeh, Innocentia, Ngong, Abong, Raphael Awah, Fombad, Fanny Fri, Njouendou, Abdel Jelil, Nietcho, Franck, Ekanya, Relindis, Kien, Chi Anizette, Ebai, Rene, Lenz, Benjamin, Ritter, Manuel, Esum, Mathias Eyong, Deribe, Kebede, Cho, Jerome Fru, Beng, Amuam Andrew, Enyong, Peter Ivo, Li, Zhiru, Hübner, Marc P., Pfarr, Kenneth, Hoerauf, Achim, Carlow, Clotilde, Wanji, Samuel“…Simulium spp. were dissected for microscopy and pooled for LAMP. The O-150 LAMP assay infection rate was 4.3% versus 4.1% by microscopy. …”
Publicado 2023
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774por Michalak, Ewa, Franaszczyk, Maria, Sobieszczanska-Malek, Malgorzata, Karcz, Maciej, Szymanska, Sylwia, Szczygiel, Justyna, Lutynska, Anna, Ploski, Rafal, Bilinska, Zofia T.Enlace del recurso
Publicado 2019
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775por Yongkiettrakul, Suganya, Kolié, Fassou René, Kongkasuriyachai, Darin, Sattabongkot, Jetsumon, Nguitragool, Wang, Nawattanapaibool, Namfon, Suansomjit, Chayanut, Warit, Saradee, Kangwanrangsan, Niwat, Buates, Sureemas“…In this present study, the PfSNP-LAMP-LFD was validated on 128 clinical malaria samples of broad ranged parasite densities (10 to 87,634 parasites per microliter of blood). …”
Publicado 2020
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776“…After analysis of the sequences of PCR amplicons, LAMP primers were generated. The optimum temperature and time for the LAMP assay were determined using eight samples—after which, the effectiveness and reproducibility of LAMP were verified by testing 40 samples, which included PCR-positive and negative samples. …”
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777por Mee, Peter T., Wong, Shani, O’Riley, Kim J., da Conceição, Felisiano, Bendita da Costa Jong, Joanita, Phillips, Dianne E., Rodoni, Brendan C., Rawlin, Grant T., Lynch, Stacey E.“…Loop-mediated isothermal amplification (LAMP) is a rapid point of care test that can overcome a range of inhibitors. …”
Publicado 2020
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778“…Finally, we demonstrated that MDV gB colocalizes with cholesterol and LAMP-1, suggesting that viral protein trafficking is mediated by LAMP-1-positive vesicles in association with cholesterol. …”
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779“…OUTCOMES: The images of choroid metastasis were recorded by the smartphone with the interface eyepiece adapter mounted on the slit lamp handily. CONCLUSIONS: The smartphone with an interface eyepiece adapter mounted on the slit lamp can be widely used to record the precious images in the clinic in a timely manner.…”
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780“…BACKGROUND: As an alternative to PCR methods, LAMP is increasingly being used in the field of molecular diagnostics. …”
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