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801por Li, Chao, Chen, Xuan, Wen, Renqiao, Ma, Peng, Gu, Kui, Li, Cui, Zhou, Changyu, Lei, Changwei, Tang, Yizhi, Wang, Hongning“…After the LAMP reaction, LAMP products were mixed and detected by the CRISPR/Cas12a cleavage mixture. …”
Publicado 2022
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802por Ren, Qian, Yu, Nuo, Zou, Peng, He, Qiang, Macharia, Daniel K., Sheng, Yangyi, Zhu, Bo, Lin, Ying, Wu, Guoyi, Chen, Zhigang“…Under the irradiation with an infrared baking lamp (IR lamp, 50 mW cm(−2)), the surface temperature of SNF-Cu(2-x)S layer on masks can quickly increase to over 78 °C, resulting from the high photothermal effects of Cu(2-x)S nanocrystals. …”
Publicado 2022
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803por Arbaciauskaite, Skaiste, Babakhani, Pouya, Sandetskaya, Natalia, Vitkus, Dalius, Jancoriene, Ligita, Karosiene, Dovile, Karciauskaite, Dovile, Zablockiene, Birute, Kuhlmeier, Dirk“…We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples. …”
Publicado 2022
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804por Zhang, Fang, Gao, Chenshan, Bai, Linlin, Chen, Yiquan, Liang, Shuying, Lv, Xucong, Sun, Jinyuan, Wang, Shaoyun“…Its prevention relies heavily on the effective avoidance of any contaminated food, making clear and accurate detection very important. LAMP is one of the most potent methods for allergen rapid detection. …”
Publicado 2021
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805por Ye, Haihang, Nowak, Chance, Liu, Yaning, Li, Yi, Zhang, Tingting, Bleris, Leonidas, Qin, Zhenpeng“…Herein, a highly specific and sensitive assay relying on plasmonic sensing of LAMP amplicons via DNA hybridization, termed as plasmonic LAMP, is developed for the severe acute respiratory syndrome‐related coronavirus 2 (SARS‐CoV‐2) RNA detection. …”
Publicado 2022
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806por Gaber, Mona, Ahmad, Alzahraa Abdelraouf, El-Kady, Asmaa M., Tolba, Mohammed, Suzuki, Yutaka, Mohammed, Shereen M., Elossily, Nahed Ahmed“…However, higher detection rates were observed with RT-LAMP assay of 60.8% and 44.4% for humans and vector mosquitoes, respectively. …”
Publicado 2022
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807por Warneford-Thomson, Robert, Shah, Parisha P, Lundgren, Patrick, Lerner, Jonathan, Morgan, Jason, Davila, Antonio, Abella, Benjamin S, Zaret, Kenneth, Schug, Jonathan, Jain, Rajan, Thaiss, Christoph A, Bonasio, Roberto“…Here, we describe COV-ID, an approach that combines RT-LAMP with deep sequencing to detect SARS-CoV-2 in unprocessed human saliva with a low limit of detection (5–10 virions). …”
Publicado 2022
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808por dos Santos, Carlos Abelardo, Silva, Lívia do Carmo, Souza Júnior, Marcio Neres de, Mendes, Geovana de Melo, Estrela, Paulo Felipe Neves, de Oliveira, Kézia Gomes, de Curcio, Juliana Santana, Resende, Paola Cristina, Siqueira, Marilda Mendonça, Pauvolid-Corrêa, Alex, Duarte, Gabriela Rodrigues Mendes, Silveira-Lacerda, Elisângela de Paula“…As a proof of concept that can help distinguish variants, we present an RT-LAMP assay capable of detecting samples carrying a group of mutations that can be related to specific SARS-CoV-2 lineages, here demonstrated for the Variant of Concern Gamma. …”
Publicado 2022
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809por Starkie, Melissa L., Fowler, Elizabeth V., Zhu, Xiaocheng, Agarwal, Arati, Rako, Lea, Schneider, Isarena C., Schutze, Mark K., Royer, Jane E., Gopurenko, David, Gillespie, Peter, Blacket, Mark J.“…A quicker, simpler, molecular diagnostic assay would facilitate a more rapid detection and potential incursion response. We developed LAMP assays targeting cytochrome c oxidase subunit I (COI) and Eukaryotic Translation Initiation Factor 3 Subunit L (EIF3L); both assays detected B. trivialis within 25 min. …”
Publicado 2022
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810por Kline, Enos C., Panpradist, Nuttada, Hull, Ian T., Wang, Qin, Oreskovic, Amy K., Han, Peter D., Starita, Lea M., Lutz, Barry R.Enlace del recurso
Publicado 2022
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811“…We developed a novel DNA based loop-mediated isothermal amplification (LAMP) assay to detect C. brevis and differentiate it from other drywood termites. …”
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812por Alhamid, Galyah, Tombuloglu, Huseyin, Motabagani, Dalal, Motabagani, Dana, Rabaan, Ali A., Unver, Kubra, Dorado, Gabriel, Al-Suhaimi, Ebtesam, Unver, Turgay“…This study describes the analytical and clinical evaluation of RT-LAMP assay for detection of SARS-CoV-2, by designing LAMP primers targeting N (nucleocapsid phosphoprotein), RdRp (polyprotein), S (surface glycoprotein), and E (envelope protein) genes. …”
Publicado 2022
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813por Son, Ki Young, Ko, Jongwoo, Kim, Eunseok, Lee, Si Young, Kim, Min-Ji, Han, Jisang, Shin, Eunhae, Chung, Tae-Young, Lim, Dong Hui“…DESIGN: Cross-sectional study in which a convolutional neural network was trained and tested using photographs of slit-lamp and retroillumination lens photographs. PARTICIPANTS: One thousand three hundred thirty-five slit-lamp images and 637 retroillumination lens images from 596 patients. …”
Publicado 2022
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814por Alvarez-Serna, Bryan E., Ramírez-Chavarría, Roberto G., Castillo-Villanueva, Elizabeth, Carrillo-Reyes, Julián, Ramírez-Zamora, Rosa María, Buitrón, Germán, Alvarez-Icaza, Luis“…The detection relies upon pH changes, due to the RT-LAMP reaction products, which are detected through a simple, but effective, extended-gate FET sensor (EGFET). …”
Publicado 2023
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815por DeLude, Anuhea, Wells, Riley, Boomla, Sherine, Chuang, Shu-Cheng, Urena, Frank, Shipman, Aaron, Rubas, Noelle, Kuehu, Donna Lee, Bickerton, Buster, Peterson, Taylor, Dobhal, Shefali, Arizala, Dario, Klair, Diksha, Ochoa-Corona, Francisco, Ali, Md Emran, Odani, Jenee, Bingham, Jon-Paul, Jenkins, Daniel M., Fletcher, Jacqueline, Stack, James P., Alvarez, Anne M., Arif, Mohammad“…Therefore, we developed a field-deployable loop-mediated isothermal amplification (LAMP) assay using a unique genomic region, present exclusively in D. fangzhongdai. …”
Publicado 2022
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816por Hurtado, Leidy, Díaz, Diana, Escorcia, Katherine, Flórez, Laura, Bello, Yesit, Díaz, Yirys, Navarro, Elkin, Pacheco, Leonardo C., Galán, Nataly, Maestre, Ronald, Acosta, Antonio, Pacheco, Lisandro A.“…RT-LAMP was performed using both extracted RNA samples and raw samples without prior RNA extraction, and the result was assessed by a simple color change in the reaction. …”
Publicado 2022
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817por Fang, Xiaoling, Deshmukh, Mihir, Chee, Miao Li, Soh, Zhi-Da, Teo, Zhen Ling, Thakur, Sahil, Goh, Jocelyn Hui Lin, Liu, Yu-Chi, Husain, Rahat, Mehta, Jodhbir, Wong, Tien Yin, Cheng, Ching-Yu, Rim, Tyler Hyungtaek, Tham, Yih-Chung“…BACKGROUND/AIMS: To evaluate the performances of deep learning (DL) algorithms for detection of presence and extent pterygium, based on colour anterior segment photographs (ASPs) taken from slit-lamp and hand-held cameras. METHODS: Referable pterygium was defined as having extension towards the cornea from the limbus of >2.50 mm or base width at the limbus of >5.00 mm. 2503 images from the Singapore Epidemiology of Eye Diseases (SEED) study were used as the development set. …”
Publicado 2022
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818por Areekit, Supatra, Tangjitrungrot, Pongbun, Khuchareontaworn, Sintawee, Rattanathanawan, Kankanit, Jaratsing, Pornpun, Yasawong, Montri, Chansiri, Gaysorn, Viseshakul, Nareerat, Chansiri, Kosum“…The limit of detection (LOD) values of the duplex LAMP-LFD for the detection of PEDV and PCV2 were 0.1 ng/µL and 0.246 ng/µL, respectively. …”
Publicado 2022
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819“…Here, we report a high-quality transparent electrode produced with an extremely low thermal budget using Xe-flash lamp rapid photonic curing. Photonic curing is an extremely short time (~ μs) process, making it possible to induce an annealing effect of over 800 °C. …”
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820por Fu, Han‐Yu, Cui, Yang, Li, Qiao, Wang, Ding, Li, Hui, Yang, Long, Wang, De‐Juan, Zhou, Jing‐Wei“…Lysosomal‐associated membrane protein type‐2A (LAMP‐2A) is a key protein in chaperone‐mediated autophagy (CMA). …”
Publicado 2022
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