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1001“…Lassa virus (LASV) cell entry is mediated by the interaction of the virus glycoprotein complex (GPC) with alpha-dystroglycan at the cell surface followed by binding to LAMP1 in late endosomes. However, LAMP1 is not absolutely required for LASV fusion, as this virus can infect LAMP1-deficient cells. …”
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1002“…H. influenzae-ERT-LAMP outcomes were in total agreement with LAMP-LFB and PCR. …”
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1003por Yip, Cyril Chik-Yan, Sridhar, Siddharth, Chan, Wan-Mui, Ip, Jonathan Daniel, Chu, Allen Wing-Ho, Leung, Kit-Hang, Cheng, Vincent Chi-Chung, Yuen, Kwok-Yung, To, Kelvin Kai-Wang“…RT-LAMP-CRISPR is fast and affordable, does not require bulky thermocyclers, and minimizes carryover contamination risk. …”
Publicado 2022
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1004por Guan, Lichun, Yu, Ziqin, Che, Zhimei, Zhang, Hang, Yu, Yong, Yang, Dicheng, Qian, Dewei, Chen, Ruizhen, Yu, Min“…Co‐overexpression of Atg5 and LAMP2 reduced myocardial injury and normalized autophagic flux. …”
Publicado 2022
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1005por Barndt, Robert J., Liu, Qing, Tang, Ying, Haugh, Michael P., Cui, Jeffery, Chan, Stephen Y., Wu, Haodi“…In the current study, we generated LAMP2 knockout (KO) human iPSC-derived cardiomyocytes (CM), which mimic the LAMP2 dysfunction in DD heart. …”
Publicado 2022
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1006“…A rapid and intuitive method for detecting Vibrio parahaemolyticus (VP) was established by a designed reaction vessel which coupled CRISPR/Cas12a with loop-mediated isothermal nucleic acid amplification (LAMP). There were two spaces in the vessel-holding LAMP reaction solution and CRISPR reaction solution, respectively, which were separated with a polyvinyl alcohol (PVA) membrane. …”
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1007por Hu, Yong-Liang, Zhang, Lian-Qing, Liu, Xiao-Qian, Ye, Wei, Zhao, Yue-Xi, Zhang, Liang, Qiang, Zun-Xian, Zhang, Lin-Xuan, Lei, Ying-Feng, Jiang, Dong-Bo, Cheng, Lin-Feng, Zhang, Fang-Lin“…The mice vaccinated with pVAX-LAMP1-CCHFV-Gc mainly elicited specific anti-Gc and neutralizing antibodies and provided a certain protection from CCHFV tecVLPs infection, but the protective efficacy was less than that of pVAX-LAMP1-CCHFV-NP. …”
Publicado 2023
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1008por Nguyen, Trieu, Vinayaka, Aaydha Chidambara, Huynh, Van Ngoc, Linh, Quyen Than, Andreasen, Sune Zoëga, Golabi, Mohsen, Bang, Dang Duong, Møller, Jens Kjølseth, Wolff, Anders“…We present in this comprehensive work, for the first time, detailed fabrication and clinical validation of a point of care (PoC) device for rapid, onsite detection of SARS-CoV-2 using a real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reaction on a polymer cartridge. The PoC system, namely PATHPOD, consisting of a standalone device (weight less than 1.2 kg) and a cartridge, can perform the detection of 10 different samples and two controls in less than 50 min, which is much more rapid than the golden standard real-time reverse-transcription Polymerase Chain Reaction (RT-PCR), typically taking 16–48 h. …”
Publicado 2023
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1009por Wang, Yongxiang, Bai, Ming, Zhang, Piyi, Peng, Yu, Chen, Zixian, He, Zhiyu, Xu, Jin, Zhu, Youqi, Yan, Dongdong, Wang, Runqing, Zhang, Zheng“…The patient carried a novel de novo mutation, LAMP2 c.2T>C located at the initiation codon. The quantitative polymerase chain reaction (qPCR) and Western blot (WB) analysis of peripheral blood leukocytes from the patients revealed evidence of LAMP2 haploinsufficiency. …”
Publicado 2023
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1010por Werbajh, Santiago, Larocca, Luciana, Carrillo, Carolina, Stolowicz, Fabiana, Ogas, Lorena, Pallotto, Sergio, Cassará, Solange, Mammana, Liliana, Zapiola, Inés, Bouzas, María Belén, Vojnov, Adrian A.“…The kit utilizes three sets of LAMP primers targeting two regions of ORF1ab and one region in the E gene. …”
Publicado 2023
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1011por Amodeo, Davide, Manzi, Pietro, De Palma, Isa, Puccio, Alessandro, Nante, Nicola, Barcaccia, Mariella, Marini, Daniele, Pietrella, Donatella“…The aim of this study was to evaluate the antimicrobial effectiveness of violet-blue (405 nm) LED lamps on high-contact surfaces in a hospital infection-control laboratory. …”
Publicado 2023
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1012por Su, Yan, Romeu-Bonilla, Eliezer, Anagnostou, Athanasia, Fitz-Patrick, David, Hearl, William, Heiland, Teri“…We designed a DNA vaccine encoding CryJ2 allergen from the JRC pollen and Lysosomal Associated Membrane Protein 1 (LAMP-1) to treat JRC allergy. These Phase IA and IB trials assessed safety and immunological effects of the investigational CryJ2-LAMP DNA vaccine in both non-sensitive and sensitive Japanese expatriates living in Honolulu, Hawaii. …”
Publicado 2017
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1013por Gandasegui, Javier, Fernández-Soto, Pedro, Muro, Antonio, Simões Barbosa, Constança, Lopes de Melo, Fabio, Loyo, Rodrigo, de Souza Gomes, Elainne Christine“…The amplification efficiency of LAMP is known to be higher than PCR. The present work aimed to assess the utility of our previously described SmMIT-LAMP assay for S. mansoni detection in human stool and snail samples in a low-transmission area of schistosomiasis in the municipality of Umbuzeiro, Paraíba State, Northeast Region of Brazil. …”
Publicado 2018
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1014por Manajit, Orapan, Longyant, Siwaporn, Sithigorngul, Paisarn, Chaivisuthangkura, Parin“…UDG-LAMP was performed to prevent carry over contamination and the LAMP reactions can be readily observed using the nanogold probe. …”
Publicado 2018
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1015“…The sensitivities and PPV were 40.91% and 90% respectively for RDTs, 72.73% and 100% respectively for PCR with LAMP as the golden standard. In low malaria transmission settings, LAMP can be also be considered for use as a surveillance tool to detect all sources of malaria and determine proportion of low parasitaemia infections in order to eliminate them.…”
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1016por Fernández-Soto, Pedro, Celis-Giraldo, Carmen T., Collar-Fernández, Coralina, Gorgojo, Óscar, Camargo, Milena, Muñoz, José, Salas-Coronas, Joaquín, Patarroyo, Manuel A., Muro, Antonio“…This study was thus aimed at comparing, for the first time, the use of a new loop-mediated isothermal amplification (LAMP) molecular assay (Strong-LAMP) to traditional methods on patients' urine samples. …”
Publicado 2020
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1017por Liao, Wenjian, Long, Dan, Huang, Qisen, Wei, Dandan, Liu, Xiaobing, Wan, Lagen, Feng, Yuling, Zhang, Wei, Liu, Yang“…OBJECTIVES: To establish a rapid molecular diagnostics of hvKp using the peg-344 loop-mediated isothermal amplification technique (LAMP). METHODS: In all, 28 K. pneumoniae strains isolated from the blood of patients were used for the peg-344 LAMP. …”
Publicado 2020
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1018por Moonga, Lavel Chinyama, Hayashida, Kyoko, Kawai, Naoko, Nakao, Ryo, Sugimoto, Chihiro, Namangala, Boniface, Yamagishi, Junya“…Aiming at rapid, simple, and simultaneous detection of SFG Rickettsia spp. and Plasmodium spp., we developed a novel multiple pathogen detection system by combining a loop-mediated isothermal amplification (LAMP) method and dipstick DNA chromatography technology. …”
Publicado 2020
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1019por Zebaze Temgoua Kemleu, Sylvie Georgette, Ngando, Laure, Nguekeng, Elvige, Fogang, Balotin, Mafo Kapen, Marie, Fopa, Styve Iruch, Biabi, Marie Florence, Essangui, Estelle, Assob Nguedia, Jules Clement, Ayong, Lawrence“…The overall concordance between the three methods were 75.9% for RT-LAMP and light microscopy, 75.1% for RT-LAMP and RDT, and 83.9% for light microscopy and RDT. …”
Publicado 2021
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1020por da Silva, Severino Jefferson Ribeiro, Pardee, Keith, Balasuriya, Udeni B. R., Pena, Lindomar“…We have previously developed and validated a one-step assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of the Zika virus (ZIKV) from mosquito samples. …”
Publicado 2021
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