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1041por Hoffmann, Elias da Rosa, Balzan, Lisiane da Rocha, Inamine, Everton, Pancotto, Lisiane Rech, Gaboardi, Guilherme, Cantarelli, Vlademir Vicente“…The results obtained with RT-LAMP (Reverse Transcriptase) were compared to the results of real-time polymerase chain reaction (RT-PCR) to assess its sensitivity and specificity. …”
Publicado 2023
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1042“…FITC was enzymatically incorporated into the RT-LAMP amplicon with the LF-LAMP primer, and biotin was introduced using biotinylated DNA probes, specifically for the amplicon region after RT-LAMP amplification. …”
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1043por Héma, Alimatou, Sermé, Samuel S., Sawadogo, Jean, Diarra, Amidou, Barry, Aissata, Ouédraogo, Amidou Z., Nébié, Issa, Tiono, Alfred B., Houard, Sophie, Chakraborty, Subhra, Ouédraogo, Alphonse, Sirima, Sodiomon B.“…The estimates of enterotoxigenic Escherichia coli (ETEC) and Shigella burden in developing countries are limited by the lack of rapid, accessible, and sensitive diagnostics and surveillance tools. We used a “Rapid LAMP based Diagnostic Test (RLDT)” to detect ETEC and Shigella in diarrheal and non-diarrheal stool samples from a 12-month longitudinal cohort of children under five years of age in a peri-urban area of Ouagadougou in Burkina Faso (West Africa). …”
Publicado 2023
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1044“…We investigated the subcellular distribution of chimeras comprised of the lumenal domain of avian LAMP-1 and the alternatively spliced domains of avian LAMP-2. …”
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1045por Godinho, Rodrigo Maciel da Costa, Matassoli, Flavio Lemos, Lucas, Carolina Gonçalves de Oliveira, Rigato, Paula Ordonhez, Gonçalves, Jorge Luiz Santos, Sato, Maria Notomi, Maciel, Milton, Peçanha, Ligia Maria Torres, August, J. Thomas, de Azevedo Marques, Ernesto Torres, de Arruda, Luciana Barros“…Cells transfected with plasmid DNA constructs containing p55gag attached to truncated sequences of LAMP-1 showed that the increased expression of gag mRNA required p55gag in frame with at least 741 bp of the LAMP-1 luminal domain. …”
Publicado 2014
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1047por Oriero, Eniyou C., Okebe, Joseph, Jacobs, Jan, Van geertruyden, Jean-Pierre, Nwakanma, Davis, D’Alessandro, Umberto“…Compared to the reference PCR method, sensitivity was 92 % for LAMP, 78 % for microscopy, and 76 % for RDT; specificity was 97 % for LAMP, 99 % for microscopy, and 88 % for RDT. …”
Publicado 2015
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1048por Badolo, Athanase, Bando, Hironori, Traoré, Alphonse, Ko-ketsu, Mami, Guelbeogo, Wamdaogo Moussa, Kanuka, Hirotaka, Ranson, Hilary, Sagnon, N’Falé, Fukumoto, Shinya“…RESULTS: The primers designed for LAMP were able to distinguish between the wild type (ace-1(S)) and mutated type allele (ace-1(R)). …”
Publicado 2015
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1049por Khorosheva, Eugenia M., Karymov, Mikhail A., Selck, David A., Ismagilov, Rustem F.“…Our finding was supported experimentally with examples of primer design, the use or exclusion of loop primers in different combinations, and the use of different enzyme mixtures in one-step reverse-transcription loop-mediated amplification (RT-LAMP). Our results show that measuring the digital efficiency of amplification of single-template molecules allows quick, reliable comparisons of the analytical sensitivity of reactions under any two tested conditions, independent of the speeds of the isothermal amplification reactions.…”
Publicado 2016
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1050por Kurosaki, Yohei, Magassouba, N’Faly, Oloniniyi, Olamide K., Cherif, Mahamoud S., Sakabe, Saori, Takada, Ayato, Hirayama, Kenji, Yasuda, Jiro“…The RT-LAMP assay is based on primer sets that target the untranscribed trailer region or nucleoprotein coding region of the viral RNA. …”
Publicado 2016
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1051por Bentaleb, El Mehdi, Abid, Mohammed, El Messaoudi, My Driss, Lakssir, Brahim, Ressami, El Mostafa, Amzazi, Saaïd, Sefrioui, Hassan, Ait Benhassou, Hassan“…CONCLUSION: To our knowledge, this is the first study using a single step LAMP (SS-LAMP) procedure as a rapid, easy to perform and cost effective testing for TB early detection. …”
Publicado 2016
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1052por Heers, Teresa, van Neer, Abbo, Becker, André, Grilo, Miguel Luca, Siebert, Ursula, Abdulmawjood, Amir“…Therefore, a loop-mediated isothermal amplification (LAMP) assay (target sequence cytochrome b) was developed to detect red fox DNA in carcasses of harbour porpoises. …”
Publicado 2017
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1053por Blaser, Simon, Diem, Hanspeter, von Felten, Andreas, Gueuning, Morgan, Andreou, Michael, Boonham, Neil, Tomlinson, Jennifer, Müller, Pie, Utzinger, Jürg, Frey, Jürg E, Bühlmann, Andreas“…CONCLUSION: Our LAMP assays reliably differentiated between the tested regulated and non‐regulated insect species within <1 h. …”
Publicado 2018
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1054por Ahn, Su Jeong, Baek, Yun Hee, Lloren, Khristine Kaith S., Choi, Won-Suk, Jeong, Ju Hwan, Antigua, Khristine Joy C., Kwon, Hyeok-il, Park, Su-Jin, Kim, Eun-Ha, Kim, Young-il, Si, Young-Jae, Hong, Seung Bok, Shin, Kyeong Seob, Chun, Sungkun, Choi, Young Ki, Song, Min-Suk“…We also evaluated this multiplex RT-LAMP for clinical use using a total of 135 clinical and spiked samples (91 influenza viruses and 44 other human infectious viruses). …”
Publicado 2019
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1055por Fgaier, Salah, de Almeida Lopes, Mônica Maria, de Oliveira Silva, Ebenézer, Aarrouf, Jawad, Urban, Laurent“…One (1P), two (2P), three (3P) or four (4P) pulses of light supplied by a xenon lamp, were applied to young lettuce plants grown in pots. …”
Publicado 2019
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1056por Hirouchi, Takatomo, Ota, Shinichiro, Mashima, Dai, Boku, Ryuichi, Yoshida, Yuto, Iwata, Koki, Shinada, Kanako, Matsumoto, Takashi, Morikawa, Miwa, Sato, Takashi, Shinoda, Masahiro, Kamachi, Kenichi, Shinkai, Masaharu“…SARS-CoV-2 RNA was detected in serum by the loop-mediated isothermal amplification (LAMP) method on Day 9; favipiravir treatment was continued. …”
Publicado 2021
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1057por Lim, Wei Sing, Grimaldi, Gabriela, Nicholson, Luke, Basheer, Khadijah, Rajendram, Ranjan“…Agreement of DR grading between both virtual graders and slit lamp examination was fair (Kappa’s coefficient = 0.56). …”
Publicado 2020
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1058por Ahn, Su Jeong, Baek, Yun Hee, Lloren, Khristine Kaith S., Choi, Won-Suk, Jeong, Ju Hwan, Antigua, Khristine Joy C., Kwon, Hyeok-il, Park, Su-Jin, Kim, Eun-Ha, Kim, Young-il, Si, Young-Jae, Hong, Seung Bok, Shin, Kyeong Seob, Chun, Sungkun, Choi, Young Ki, Song, Min-SukEnlace del recurso
Publicado 2020
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1059por Allgöwer, Stefanie M., Hartmann, Chris A., Lipinski, Clarissa, Mahler, Vera, Randow, Stefanie, Völker, Elke, Holzhauser, Thomas“…Sensitivity of soybean detection in incurred food matrices, commercial retail samples, as well as various processed soybean products was comparable between LAMP-LFD and qPCR. The DNA-based LAMP-LFD proved to be a simple and low-technology soybean detection tool, showing sensitivity and specificity that is comparable or superior to the investigated commercial protein-based LFD.…”
Publicado 2020
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1060por Upadhyaya, Swati, Agarwal, Anushri, Rengaraj, Venkatesh, Srinivasan, Kavitha, Newman Casey, Paula Anne, Schehlein, EmilyEnlace del recurso
Publicado 2021
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