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1101por Natoli, Mary, Kundrod, Kathryn, Chang, Megan, Smith, Chelsey, Paul, Sai, Eldin, Karen, Patel, Keyur, Baker, Ellen, Schmeler, Kathleen, Richards-Kortum, Rebecca“…FINDINGS: The developed RT-LAMP assay demonstrated a limit of detection of 4–5 virions/μL. …”
Publicado 2021
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1102por Zhang, Yaqin, Chen, Minyan, Liu, Chengrong, Chen, Jiaqi, Luo, Xinyi, Xue, Yingying, Liang, Qiming, Zhou, Li, Tao, Yu, Li, Mingqiang, Wang, Di, Zhou, Jianhua, Wang, Jiasi“…We here developed a gold nanoparticle-based visual assay that combines with CRISPR/Cas12a-assisted RT-LAMP, which is called Cas12a-assisted RT-LAMP/AuNP (CLAP) assay for rapid and sensitive detection of SARS-CoV-2. …”
Publicado 2021
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1103por Lee, In Hee, Jung, Jin Hyang, Lee, Soo Jung, Lee, Jeeyeon, Park, Ho Yong, Park, Ji-Young, Park, Jee Young, Jung, Jae-hwan, Lee, Hyunchul, Jeon, Hyo-Sung, Chae, Yee Soo“…Because a direct reverse transcription loop-mediated isothermal amplification method (direct RT-LAMP) has been previously developed in order to rapidly identify viruses in place of RNA extraction, our team hypothesized that a direct RT-LAMP assay can be employed as a substitute in order to detect tumor involvement of lymph nodes within breast cancer patients. …”
Publicado 2022
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1104por Sharma, Supriya, Kumar, Sandeep, Ahmed, Md Zohaib, Bhardwaj, Nitin, Singh, Jaskirat, Kumari, Sarita, Savargaonkar, Deepali, Anvikar, Anupkumar R., Das, Jyoti“…Melting curve analysis of mLAMP revealed the lowest detection limit of 0.15 pg/μL from sample genomic DNA. …”
Publicado 2021
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1105por Abelardo dos Santos, Carlos, Silva, Lívia do Carmo, Júnior, Marcio Neres de Souza, Mendes, Geovana de Melo, de Curcio, Juliana Santana, Estrela, Paulo Felipe Neves, de Oliveira, Kézia Gomes, Mendes Duarte, Gabriela Rodrigues, Silveira-Lacerda, Elisângela de Paula“…A técnica de Amplificação Isotérmica Mediada por Loop com transcriptase reversa (RT-LAMP) é uma técnica barata, de fácil aplicação e com potencial de distinguir amostras que contenham pequenas regiões com mutações e/ou SNPs. …”
Publicado 2022
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1106por Chiu, Chien-Chih, Chen, Yen-Chun, Bow, Yung-Ding, Chen, Jeff Yi-Fu, Liu, Wangta, Huang, Jau-Ling, Shu, En-De, Teng, Yen-Ni, Wu, Chang-Yi, Chang, Wen-Tsan“…Furthermore, insufficient LAMP2 glycosylation induced by diTFPP/ceramide cotreatment may cause the failure of autophagosome–lysosome fusion, eventually lowering the threshold for triggering cell death in response to C(2)-ceramide. …”
Publicado 2022
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1107por Wanney, Johannes, Lüsebrink, Jessica, Spölgen, Gina, Demuth, Sabrina, Schildgen, Verena, Schildgen, Oliver“…The positive and negative likelihood ratio for LAMP was 6.693 and 0.241, respectively, while the diagnostic odds ratio was 27.77. …”
Publicado 2022
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1109por Wang, Huanan, Cong, Feng, Zeng, Fanwen, Lian, Yuexiao, Liu, Xiangnan, Luo, Manlin, Guo, Pengju, Ma, Jingyun“…A simple and rapid detection test was developed for this virus using real-time RT-LAMP based on the conserved N gene of the virus. …”
Publicado 2018
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1110por Gachugia, James, Chebore, Winnie, Otieno, Kephas, Ngugi, Caroline Wangari, Godana, Adano, Kariuki, Simon“…At Siaya hospital the sensitivity of routine microscopy and MG-LAMP was 83.3% (CI 65.3–94.4) and 93.3% (CI 77.9–99.2), respectively. …”
Publicado 2020
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1111por Haq, Farhan, Sharif, Salmaan, Khurshid, Adnan, Ikram, Aamer, Shabbir, Imran, Salman, Muhammad, Ahad, Abdul, Suleman Rana, Muhammad, Raja, Aroosha, Badar, Nazish, Tashkandi, Hanaa, Al Amri, Turki, Azhar, Esam I., Almuhayawi, Mohammed S., Harakeh, Steve, Faraz Arshad Malik, Muhammad“…For this purpose, a cohort of 297 suspected COVID-19 patients was tested using both the RT-LAMP assay and the conventional RT-PCR method. For the RT-LAMP assay, three genes (orf-1ab, N, and S) were identified as the target sites for the detection of COVID-19. …”
Publicado 2021
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1112por Castellucci, Léa C, Almeida, Lucas, Cherlin, Svetlana, Fakiola, Michaela, Francis, Richard W, Carvalho, Edgar M, Santos da Hora, Anadílton, do Lago, Tainã Souza, Figueiredo, Amanda B, Cavalcanti, Clara M, Alves, Natalia S, Morais, Katia L P, Teixeira-Carvalho, Andréa, Dutra, Walderez O, Gollob, Kenneth J, Cordell, Heather J, Blackwell, Jenefer M“…LAMP3 (P(adjusted) = 9.25 × 10(−12); +6-fold), STX7 (P(adjusted) = 7.62 × 10(−3); +1.3-fold), and CRLF3 (P(adjusted) = 9.19 × 10(−9); +1.97-fold) were expressed more highly in CL biopsies compared to normal skin; KRT80 (P(adjusted) = 3.07 × 10(−8); −3-fold) was lower. …”
Publicado 2020
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1113“…Loop-mediated isothermal amplification or LAMP is one of the DNA analysis techniques used in forensic study. …”
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1114“…We found a significant divergence of grade value distribution between cancers of different DCs’ CD1a+ counts. The DC-LAMP+ count was positively associated with grade. …”
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1115por Cao, Yumeng, Lu, Xiao, Lin, Haosi, Rodriguez Serrano, Alan Fernando, Lui, Grace C.Y., Hsing, I-Ming“…Here, we report an amplicon-depleting CRISPR-based one-pot loop-mediated isothermal amplification (CoLAMP) assay for point-of-care diagnosis of SARS-CoV-2 RNA. …”
Publicado 2023
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1116por Iijima, Takahiro, Sakai, Jun, Kanamori, Dai, Ando, Shinnosuke, Nomura, Tsutomu, Tisi, Laurence, Kilgore, Paul E., Percy, Neil, Kohase, Hikaru, Hayakawa, Satoshi, Maesaki, Shigefumi, Hoshino, Tomonori, Seki, Mitsuko“…In this study, we established LAMP primers and peptide nucleic acid probes to detect N501Y and Q493R/Q498R. …”
Publicado 2023
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1117por McCreesh, Patrick, Mumbengegwi, Davis, Roberts, Kathryn, Tambo, Munyaradzi, Smith, Jennifer, Whittemore, Brooke, Kelly, Gerard, Moe, Caitlin, Murphy, Max, Chisenga, Mukosha, Greenhouse, Bryan, Ntuku, Henry, Kleinschmidt, Immo, Sturrock, Hugh, Uusiku, Petrina, Gosling, Roland, Bennett, Adam, Hsiang, Michelle S.“…RESULTS: Prevalence of Plasmodium falciparum malaria infection was low: 0.8% (16/1919) by RDT and 2.2% (43/1919) by LAMP. All but one LAMP-positive infection was RDT-negative. …”
Publicado 2018
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1118por Iijima, Takahiro, Ando, Shinnosuke, Kanamori, Dai, Kuroda, Kazumichi, Nomura, Tsutomu, Tisi, Laurence, Kilgore, Paul E., Percy, Neil, Kohase, Hikaru, Hayakawa, Satoshi, Seki, Mitsuko, Hoshino, Tomonori“…The novel RT-LAMP-BART assay to detect SARS-CoV-2 was highly specific compared to the conventional real-time RT-PCR. …”
Publicado 2022
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