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1301por Lai, Guan-Hua, Chao, Jung, Lin, Ming-Kuem, Chang, Wen-Te, Peng, Wen-Huang, Sun, Fang-Chun, Lee, Meng-Shiunn, Lee, Meng-Shiou“…LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. …”
Publicado 2015
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1302“…The LAMP assay was compared with qPCR in the examination of 100 rhesus and 20 rhesus-feeder blood samples; the diagnostic accuracy was found to be 100% when LAMP was compared to qPCR, but the LAMP assay was significantly more sensitive (p < 0.05). …”
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1303por Zhang, Enming, Kircher, Moritz F., Koch, Martin, Eliasson, Lena, Goldberg, S. Nahum, Renström, Erik“…LAMP1-SPIONs then preferentially accumulated along the membrane in lysosomes in both rat insulinoma tumor cells and human pancreatic beta cells due to binding of LAMP1-SPIONs to endogenous LAMP1. …”
Publicado 2014
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1304por Xie, Guosi, Zhang, Qingli, Han, Nana, Shi, Chengyin, Wang, Xiuhua, Liu, Qinghui, Huang, Jie“…An improved method for quick and accurate detection of E. tarda by loop-mediated isothermal amplification (LAMP) with two additional loop primers was developed by targeting the EsrB gene (EsrB — LAMP). …”
Publicado 2012
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1305por Pan, Lei, Zhang, Lijuan, Fan, Desheng, Zhang, Xiuchun, Liu, Hong, Lu, Qunying, Xu, Qiyi“…RESULTS: The limits of detection of the LAMP assay for the htpAB gene were 1 copy per reaction. …”
Publicado 2013
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1306por Ahmed, Mohamed E., Eldigail, Mawahib H., Elamin, Fatima M., Ali, Ibtisam A., Grobusch, Martin P., Aradaib, Imadeldin E.“…METHODS: A set of six LAMP primers, designed from the mitochondrial NADH-1 gene of EG cattle strain of genotype 5 (G5), was used as a target for LAMP assay. …”
Publicado 2016
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1307por Zhou, Yi, Wan, Zhenzhou, Yang, Shuting, Li, Yingxue, Li, Min, Wang, Binghui, Hu, Yihong, Xia, Xueshan, Jin, Xia, Yu, Na, Zhang, Chiyu“…Loop-mediated isothermal amplification (LAMP) has been widely used in the detection of pathogens causing infectious diseases. …”
Publicado 2019
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1308por Chen, Xu, Zhou, Qingxue, Li, Shijun, Yan, Hao, Chang, Bingcheng, Wang, Yuexia, Dong, Shilei“…The sensitivity and specificity of the mRT-LAMP-LFB method were tested in the current study, and the mRT-LAMP-LFB assay was applied to detect the SARS-CoV-2 virus from clinical samples and artificial sputum samples. …”
Publicado 2021
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1309por Ramezani, Reihaneh, Kardoost Parizi, Zahra, Ghorbanmehr, Nassim, Mirshafiee, Hamideh“…LAMP assay showed the best detection limit of DNA copies with 1.02 copies. …”
Publicado 2018
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1310por Hu, Xuejiao, Deng, Qianyun, Li, Junmin, Chen, Jierong, Wang, Zixia, Zhang, Xiqin, Fang, Zhixin, Li, Haijian, Zhao, Yunhu, Yu, Pan, Li, Wenmin, Wang, Xiaoming, Li, Shan, Zhang, Lei, Hou, Tieying“…The time required for RT-LAMP was less than 1 h from sample preparation to the result. …”
Publicado 2020
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1311“…The sensitivity, specificity and reproducibility of the LAMP were evaluated. The LAMP assay for detecting SFGR was compared with conventional PCR assays for sensitivity and specificity in early phase blood samples obtained from 11 infected human subjects. …”
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1312por McElgunn, Cathal J., Pereira, Clint R., Parham, Nicholas J., Smythe, James E., Wigglesworth, Michael J., Smielewska, Anna, Parmar, Surendra A., Gandelman, Olga A., Brown, Nicholas M., Tisi, Laurence C., Curran, Martin D.“…In a further study (tcdB (+), n = 47; tcdB (−), n = 28) HE-LAMP-BART was compared to an alternative commercially available LAMP-based method, Illumigene (Meridian Bioscience, OH), and yielded 87.2% sensitivity and 100% specificity for the HE-LAMP-BART method compared to 76.6% and 100%, respectively, for Illumigene against the reference method. …”
Publicado 2014
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1313por Kudyba, Heather M., Louzada, Jaime, Ljolje, Dragan, Kudyba, Karl A., Muralidharan, Vasant, Oliveira-Ferreira, Joseli, Lucchi, Naomi W.“…Two independent readers scored the MG-LAMP tests as positive (blue/green) or negative (clear). …”
Publicado 2019
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1314por Barazorda, Keare A., Salas, Carola J., Bishop, Danett K., Lucchi, Naomi, Valdivia, Hugo O.“…Both LAMP methodologies could be used as large-scale screening tests, but the MG-LAMP assay uses a simple, portable heat-block while the RealAmp requires a RealAmp machine or a real-time PCR machine. …”
Publicado 2020
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1315“…MiR-1-3p was lowly expressed in AB, which exhibited negative correlations to LAMP2 expression. MiR-1-3p up-regulation significantly lowered LAMP2 expression in AM-1 cells. …”
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1316por Kumagai, Takashi, Matsumoto-Takahashi, Emilie Louise Akiko, Ishikawa, Hirofumi, Keomalaphet, Sengdeuane, Khattignavong, Phonepadith, Soundala, Pheovaly, Hongvanthong, Bouasy, Oyoshi, Kei, Sasaki, Yoshinobu, Mizukami, Yousei, Kano, Shigeyuki, Brey, Paul T., Iwagami, Moritoshi“…The objective of this study was to develop a LAMP assay for Schistosoma mekongi using a simple DNA extraction method. …”
Publicado 2022
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1317por Yang, Bo-Yun, Liu, Xiao-Lu, Wei, Yu-Mei, Wang, Jing-Qi, He, Xiao-Qing, Jin, Yi, Wang, Zi-Jian“…BACKGROUND: The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus. …”
Publicado 2014
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1318por Coelho, Beatriz Jorge, Veigas, Bruno, Águas, Hugo, Fortunato, Elvira, Martins, Rodrigo, Baptista, Pedro Viana, Igreja, Rui“…Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. …”
Publicado 2017
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1319“…We provided a useful technique, loop-mediated isothermal amplification (LAMP), to differentiate Guang-dilong from other species. …”
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1320por Yoshikawa, Rokusuke, Abe, Haruka, Igasaki, Yui, Negishi, Saeki, Goto, Hiroaki, Yasuda, Jiro“…Although the Direct RT-LAMP assay was less sensitive than the standard RT-LAMP, the Direct RT-LAMP assay can be available as the rapid first screening of COVID-19 in poorly equipped areas, such as rural areas in developing countries.…”
Publicado 2020
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