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1321“…In this study, we aimed to develop a rapid and sensitive point-of-care AIV test using loop-mediated isothermal amplification (LAMP) technology. We designed three sets of reverse transcription LAMP (RT-LAMP) primers targeting the matrix (M) and hemagglutinin (HA) genes of the H5 and H9 subtypes. …”
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1322Loop-Mediated Isothermal Amplification as Point-of-Care Testing for EGFR-Mutated Lung Adenocarcinomapor Saito, Yuichi, Matsui, Atsuka, Michiyuki, Satoru, Morooka, Hiroaki, Ibi, Takayuki, Yamauchi, Yoshikane, Takahashi, Nobumasa, Shimizu, Yoshihiko, Ikeya, Tomohiko, Hoshi, Eishin, Sakao, Yukinori, Kawamura, Masafumi“…Loop-mediated isothermal amplification (LAMP) has been widely used for the rapid detection of pathogens through DNA amplification. …”
Publicado 2022
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1323por Jelocnik, Martina, Islam, Md. Mominul, Madden, Danielle, Jenkins, Cheryl, Branley, James, Carver, Scott, Polkinghorne, Adam“…For a subset of 36 C. pecorum samples tested in a thermal cycler using newly developed LAMP, we observed 34/36 (94.4%) samples result being congruent between LAMP performed in fluorometer and in thermal cycler. …”
Publicado 2017
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1324“…RESULTS: A total of 123 specimens collected from suspicious patients with HFMD were simultaneously detected by RT-LAMP and PCR fluorescence probing assay. The RT-LAMP amplified products containing EV71 were digested by HinfI and TaqI restriction endonucleases; in contrast, non-specific products with CVA16, coxsackievirus A4 and coxsackievirus B3 could not be detected in RT-LAMP assay. …”
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1325por Wang, Fang, Li, Ran, Shang, Ying, Wang, Can, Wang, Guo-Qing, Zhou, De-Xun, Yang, Dong-Hong, Xi, Wen, Wang, Ke-Qiang, Bao, Jing, Kang, Yu, Gao, Zhan-Cheng“…Specific DNA fragments for identifying 11 pneumonia-related bacteria were amplified by qLAMP assay. Culture results of these patients were compared with the qLAMP results. …”
Publicado 2016
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1326por Ge, Yiyue, Zhou, Qiang, Zhao, Kangchen, Chi, Ying, Liu, Bin, Min, Xiaoyan, Shi, Zhiyang, Zou, Bingjie, Cui, Lunbiao“…However, with regard to multiplex LAMP, differentiating the ladder-like LAMP products derived from multiple targets is still challenging today. …”
Publicado 2017
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1327por Wang, Yi, Wang, Xiaoxia, Chen, Hai, Han, Limei, Wang, Licheng, Chen, Ting, Li, Sha, Li, Huan, Li, Yuanli, Li, Zhengkun, Fu, Xiaoying, Chen, Shaojin, Xing, Mei, Tai, Jun, Zhu, Xiong“…Here, we reported a novel detection platform based on the loop-mediated isothermal amplification (LAMP), termed real-time reverse transcription LAMP (rRT-LAMP) and applied it for the diagnosis of COVID-19 (COVID-19 rRT-LAMP). rRT-LAMP integrates reverse transcription, LAMP amplification, restriction endonuclease cleavage and real-time fluorescence detection into one-pot reaction, and facilitates the diagnosis of COVID-19 at 64°C for only 35 min. …”
Publicado 2021
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1328por Xiao, Fei, Zhou, Juan, Sun, Chunrong, Huang, Xiaolan, Zheng, Baoying, Fu, Jin, Jia, Nan, Xu, Zheng, Cui, Xiaodai, Wang, Yi“…Here, we designed a loop-mediated isothermal amplification (LAMP)-based assay, termed LAMP, combined with a nanoparticle-based lateral flow biosensor (LAMP-LFB) for rapid and sensitive diagnosis of MP.…”
Publicado 2022
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1329por Liu, Jing, Xu, Liping, Guo, Jinlong, Chen, Rukai, Grisham, Michael Paul, Que, Youxiong“…Based on loop-mediated isothermal amplification (LAMP), we developed a method for detecting Lxx. The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of Lxx and without the need for costly PCR apparatus and gel scanner. …”
Publicado 2013
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1330por HIGA, Yumiko, UEMURA, Ryoko, YAMAZAKI, Wataru, GOTO, Shinya, GOTO, Yoshitaka, SUEYOSHI, Masuo“…We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. …”
Publicado 2016
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1331“…Without prior cultural enrichment and DNA extraction steps, bacterial DNA was released by heating at 100°C for 5 min and directly subjected to LAMP assay. Using a set of LAMP primers recognizing six distinct regions of nuc gene, the developed direct LAMP assay was highly specific, and the analytical sensitivity was determined to be 7.6 × 10(2) CFU/mL. …”
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1332por Qiao, Ning, Dai, Huijie, Liu, Jie, Zhu, Xiaoping, Li, Jintang, Zhang, Dezhen, Liu, Yongguang“…The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV. A set of four LAMP primers was designed based on the coat protein gene sequence of MNSV, and a RT-LAMP reaction was successfully performed for 1 h at 62°C. …”
Publicado 2020
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1333Publicado 1994“…A soluble LAMP-1/immunoglobulin fusion protein (LAMP-1/Ig) was transiently expressed in both BR96 binding and nonbinding cell lines. …”
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1334“…The multiple-LAMP conditions were optimized at 63 °C for 40 min. …”
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1335por Liu, Wenwen, Lu, Gang, Wang, Yu, Chen, Zhenghong, Gao, Yunyun, Yin, Zhipeng, Wu, Yi, Lv, Xiaoqian, Guo, Pengbo, Zhao, Yinghui“…UreB-FIP-labeled biotin was used for LAMP amplification, and FAM-labeled probes were specifically hybridized with LAMP amplification products, which were then detected by LFD. …”
Publicado 2023
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1336por Hess, Julian, Kreitlow, Antonia, Rohn, Karl, Hennig-Pauka, Isabel, Abdulmawjood, Amir“…For this LAMP assay, two different methods for the extraction of DNA from brain and joint swabs were compared. …”
Publicado 2023
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1337por Wang, Cen, Chen, Lin, Yin, Xuren, Hua, Wanquan, Hou, Min, Ji, Minjun, Yu, Chuanxin, Wu, Guanling“…LAMP and conventional PCR were shown to amplify the target sequence of the SjR2-pCR2.1 recombinant plasmid template with limits of detection of 10(-4 )ng and 10(-2 )ng, respectively, thus demonstrating the superior sensitivity of the LAMP method. …”
Publicado 2011
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1338por Nie, Kai, Qi, Shun-xiang, Zhang, Yong, Luo, Le, Xie, Yun, Yang, Meng-jie, Zhang, Yi, Li, Jin, Shen, Hongwei, Li, Qi, Ma, Xue-jun“…The direct RT-LAMP assay was evaluated and compared to both RT-LAMP and quantitative real-time PCR (qRT-PCR) in detecting EV71 infection with 145 nasopharyngeal swab specimens. …”
Publicado 2012
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1339por Murray, Lee, Edwards, Lorraine, Tuppurainen, Eeva SM, Bachanek-Bankowska, Katarzyna, Oura, Chris AL, Mioulet, Valerie, King, Donald P“…RESULTS: A single LAMP assay targeting a conserved region of the CaPV P32 gene was selected from 3 pilot LAMP assays and optimised by adding loop primers to accelerate the reaction time. …”
Publicado 2013
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1340por Aydin-Schmidt, Berit, Morris, Ulrika, Ding, Xavier C., Jovel, Irina, Msellem, Mwinyi I., Bergman, Daniel, Islam, Atiqul, Ali, Abdullah S., Polley, Spencer, Gonzalez, Iveth J., Mårtensson, Andreas, Björkman, Anders“…For the PCR positive samples, there was no statistically significant difference between the geometric mean parasite densities among the HTP-LAMP positive (2.5 p/μL, range 0.2–770) and HTP-LAMP negative (1.4 p/μL, range 0.1–7) samples (p = 0.088). …”
Publicado 2017
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