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1481“…BACKGROUND: To analyze the expression and clinical significance of murine double minute 2 (MDM2), lysosome-associated membrane protein (LAMP1) and P-glycoprotein (P-gp) in children with acute lymphoblastic leukemia (ALL). …”
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1482por Qiao, Lei, Ma, Jing, Zhang, Zihao, Sui, Wenhai, Zhai, Chungang, Xu, Dan, Wang, Zunzhe, Lu, Huixia, Zhang, Meng, Zhang, Cheng, Chen, Wenqiang, Zhang, Yun“…Furthermore, gain-of-function studies verified that restoration of LAMP-2A levels in LAMP-2A–deficient macrophages greatly attenuated NLRP3 inflammasome activation. …”
Publicado 2021
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1483por Chitpim, Natthakan, Jittikoon, Jiraphun, Udomsinprasert, Wanvisa, Mahasirimongkol, Surakameth, Chaikledkaew, Usa“…METHODS: A hybrid decision tree Markov model using a 1-month cycle length was used to evaluate costs and outcomes of five TB diagnostic algorithms: 1) sputum smear microscopy (SSM) with culture and drug susceptibility testing (DST), 2) Xpert MTB/RIF add-on, 3) Xpert MTB/RIF initial, 4) TB-LAMP add-on, and 5) TB-LAMP initial during a lifetime period. …”
Publicado 2022
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1484“…The LAMP reactions were able to be completed in 60 min at a single temperature of 64◦C. …”
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1485por Shimuta, Ken, Takahashi, Hideyuki, Akeda, Yukihiro, Nakayama, Shu-ichi, Ohnishi, Makoto“…To develop a nonculture test for predicting the CFM responsiveness of N. gonorrhoeae strains, we developed an assay to detect N. gonorrhoeae nonmosaic penA using loop-mediated isothermal amplification (LAMP). To avoid false-positive reactions with commensal Neisseria spp. penA, we amplified specific regions of the N. gonorrhoeae penA (NG-penA-LAMP1) and also the nonmosaic N. gonorrhoeae penA (NG-penA-LAMP3). …”
Publicado 2022
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1486por Lai, Meng Yee, Zen, Lee Phone Youth, Abdul Hamid, Mohd Hafizi, Jelip, Jenarun, Mudin, Rose Nani, Ivan, Vun Jan Shui, Francis, Lee Ngie Ping, Saihidi, Izreena, Lau, Yee Ling“…We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detect Plasmodium knowlesi, P. vivax, P. falciparum, and Plasmodium genus (for P. malariae and P. ovale). …”
Publicado 2023
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1487“…The results of LAMP and the PCR tests showed very high agreement (k = 0.97) in the detection of the bacteria studied. …”
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1488“…METHODS: The effects of radation time, turbidity, pH and temperature were invistigated in this study. Ultraviolet lamp used in this study was a 11 W lamp and intensity of this lamp was 24 μw / cm(2). …”
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1489por Zhou, Dinggang, Wang, Chunfeng, Li, Zhu, Chen, Yun, Gao, Shiwu, Guo, Jinlong, Lu, Wenying, Su, Yachun, Xu, Liping, Que, Youxiong“…A set of six primers was designed for LAMP-based amplification of the bar gene. The LAMP reaction conditions were optimized as follows: 5.25 mM of Mg(2+), 6:1 ratio of inner vs. outer primer, and 6.0 U of Bst DNA polymerase in a reaction volume of 25.0 μL. …”
Publicado 2016
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1490por Liu, Wei, Huang, Simo, Liu, Ningwei, Dong, Derong, Yang, Zhan, Tang, Yue, Ma, Wen, He, Xiaoming, Ao, Da, Xu, Yaqing, Zou, Dayang, Huang, Liuyu“…This study established a constant-temperature fluorescence quantitative detection method, combining loop-mediated isothermal amplification (LAMP) with molecular beacons. The advantages of LAMP are its convenience and efficiency, as it does not require a thermocycler and results are easily visualized by the naked eye. …”
Publicado 2017
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1491“…Relative to traditional culture, the sensitivity and specificity of the LAMP assay were 94.7 and 85.7%, respectively. CONCLUSION: LAMP was sensitive and reliable for detecting the porA gene of N. gonorrhoeae. …”
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1492“…METHODS: Forty depressed male rats were randomly divided into five groups of 8 rats each: depressive model, microwave sulfur lamp, halogen lamp, fluorescent lamp, and LED lamp groups. …”
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1493“…Finally, the sensitivity of qPCR and LAMP assays were evaluated. The results showed that the primer Y-430 F/R, Y-307 F/R, Y-755 F/R, and Y-139 F/R for qPCR and primers L-139 and L-988 for LAMP could be used for U. tritici detection. …”
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1494por Fan, Qing, Xie, Zhixun, Xie, Liji, Liu, Jiabo, Pang, Yaoshan, Deng, Xianwen, Xie, Zhiqin, Peng, Yi, Wang, Xiuqing“…A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. …”
Publicado 2012
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1495por Ichikawa, Akihiro, Fujita, Yu, Hosaka, Yusuke, Kadota, Tsukasa, Ito, Akihiko, Yagishita, Shigehiro, Watanabe, Naoaki, Fujimoto, Shota, Kawamoto, Hironori, Saito, Nayuta, Yoshida, Masahiro, Hashimoto, Mitsuo, Minagawa, Shunsuke, Hara, Hiromichi, Motoi, Noriko, Yamamoto, Yusuke, Ochiya, Takahiro, Araya, Jun, Kuwano, Kazuyoshi“…Lysosome‐associated membrane protein type 2a (LAMP2A) is the key receptor protein of CMA; downregulation of LAMP2A leads to CMA blockade. …”
Publicado 2020
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1496“…An alternative to RT‐PCR is loop‐mediated isothermal amplification coupled with reverse transcription (RT‐LAMP) that is easy to perform, quick and does not require a thermal cycler. …”
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1497por Cao, Xiaoying, Chang, Yanbin, Tao, Chunqing, Chen, Sen, Lin, Qiuxia, Ling, Chao, Huang, Shifeng, Zhang, Hengshu“…After comparing the sensitivity and specificity of three different methods, we determined that the nuc-LAMP-Cas12a and mecA-LAMP-Cas12a methods were the optimal detection methods. …”
Publicado 2023
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1498por Kidd, Stephen P., Burns, Daniel, Armson, Bryony, Beggs, Andrew D., Howson, Emma L.A., Williams, Anthony, Snell, Gemma, Wise, Emma L., Goring, Alice, Vincent-Mistiaen, Zoe, Grippon, Seden, Sawyer, Jason, Cassar, Claire, Cross, David, Lewis, Thomas, Reid, Scott M., Rivers, Samantha, James, Joe, Skinner, Paul, Banyard, Ashley, Davies, Kerrie, Ptasinska, Anetta, Whalley, Celina, Ferguson, Jack, Bryer, Claire, Poxon, Charlie, Bosworth, Andrew, Kidd, Michael, Richter, Alex, Burton, Jane, Love, Hannah, Fouch, Sarah, Tillyer, Claire, Sowood, Amy, Patrick, Helen, Moore, Nathan, Andreou, Michael, Morant, Nick, Houghton, Rebecca, Parker, Joe, Slater-Jefferies, Joanne, Brown, Ian, Gretton, Cosima, Deans, Zandra, Porter, Deborah, Cortes, Nicholas J., Douglas, Angela, Hill, Sue L., Godfrey, Keith M., Fowler, Veronica L.“…This multisite clinical evaluation describes the validation of an improved sample preparation method for extraction-free RT-LAMP and reports clinical performance of four RT-LAMP assay formats for SARS-CoV-2 detection. …”
Publicado 2022
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1499por Karthik, K., Rathore, Rajesh, Thomas, Prasad, Arun, T.R., Viswas, K.N., Dhama, Kuldeep, Agarwal, R.K.“…Loop mediated isothermal amplification (LAMP) assay, a promising diagnostic test, has been developed for detection of different pathogens of human as well as animals. …”
Publicado 2014
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1500por Singh, Preeti, Singh, Sundeep, Mirdha, Bijay Ranjan, Guleria, Randeep, Agarwal, Sanjay Kumar, Mohan, Anant“…In the present study, the loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Pneumocystis jirovecii. 185 clinical respiratory samples, including both BALF and IS, were subjected to GMS staining, nested PCR, and LAMP assay. …”
Publicado 2015
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