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1601por Zhang, Qingli, Standish, Isaac, Winters, Andrew D., Puzach, Corey, Ulferts, Rachel, Ziebuhr, John, Faisal, Mohamed“…It is anticipated that the developed RT-LAMP and quantitative RT-LAMP methods will be instrumental for diagnosis and surveillance of FHMNV.…”
Publicado 2014
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1602por Nemoto, Manabu, Morita, Yoshinori, Niwa, Hidekazu, Bannai, Hiroshi, Tsujimura, Koji, Yamanaka, Takashi, Kondo, Takashi“…A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of equine coronavirus (ECoV). …”
Publicado 2015
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1603“…For LAMP detection, a SPR based LAMP sensing system (SPRLAMP) was constructed, including a novel SPRLAMP sensing cartridge integrating a polymethyl methacrylate (PMMA) micro-reactor with a polycarbonate (PC)-based prism coated with a 50 nm Au film. …”
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1604por Sheikh, Nazish, Kumar, Sanjay, Sharma, Harsh Kumar, Bhagyawant, Sameer S., Thavaselvam, Duraipandian“…A very high level of agreement was observed between both colorimetric LAMP and reference qPCR assay. The colorimetric LAMP assay reported here is a rapid and simple test without extensive sample preparation and has a short turnaround time of <45 min. …”
Publicado 2020
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1605por Singh, Manraj Deep, Singh, Harkirat, Singh, Nirbhay Kumar, Singh, Niraj Kumar, Sood, Naresh Kumar, Rath, Shitanshu Shekar“…PURPOSE: The study was planned to develop a simple read out loop mediated isothermal amplification (LAMP) assay targeting a partial 18S rRNA gene of H. canis with naked eye visualisation of LAMP products. …”
Publicado 2019
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1606por Aonuma, Hiroka, Iizuka-Shiota, Itoe, Hoshina, Tokio, Tajima, Shigeru, Kato, Fumihiro, Hori, Seiji, Saijo, Masayuki, Kanuka, Hirotaka“…RT-LAMP reactions using a screened primer set, targeting the NS3 region, detected both Zika virus strains. …”
Publicado 2020
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1607por Taki, Keisuke, Yokota, Isao, Fukumoto, Tatsuya, Iwasaki, Sumio, Fujisawa, Shinichi, Takahashi, Masayoshi, Negishi, Saeki, Hayasaka, Kasumi, Sato, Kaori, Oguri, Satoshi, Nishida, Mutsumi, Sugita, Junichi, Konno, Satoshi, Saito, Tomoya, Teshima, Takanori“…Reverse transcription loop-mediated isothermal amplification (RT-LAMP) can detect SARS-CoV-2 more rapidly than RT-PCR. …”
Publicado 2021
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1608por Moeini-Zanjani, Ali, Pournajaf, Abazar, Ferdosi-Shahandashti, Elaheh, Gholami, Mehrdad, Masjedian, Faramarz, Khafri, Soraya, Rajabnia, Ramazan“…In this study, LAMP assay was comprehensively evaluated against the common PCR method. …”
Publicado 2020
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1609por Mikami, Yuta, Fuwa, Kazumasa, Arima, Eriko, Suda, Yasuo, Yanagihara, Itaru, Ibara, Satoshi“…One hundred sixty-seven nasopharyngeal swabs and 101 tracheal aspirates were analyzed by LAMP, culture, and quantitative real-time polymerase chain reaction. …”
Publicado 2021
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1610por MUNGTHONG, Kanumporn, KHAING, Soe Thiri, OTSUBO, Takehiko, HATANAKA, Chihiro, YONEYAMA, Shuji, HISAMATSU, Shin, MURAKAMI, Hironobu, TSUKAMOTO, Kenji“…For broad detection of pestivirus A (bovine viral diarrhea virus 1: BVDV1) and pestivirus B (BVDV2) by a reverse transcription loop-mediated isothermal amplification (RT-LAMP) test, the P25 primer set was designed using nucleotide sequences of 5’-UTR region of 1454 BVDVs. …”
Publicado 2021
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1611por Jones, Les, Bakre, Abhijeet, Naikare, Hemant, Kolhe, Ravindra, Sanchez, Susan, Mosley, Yung-Yi C., Tripp, Ralph A.“…When compared to RT-qPCR, positive NP swab samples assayed by fluorescent RT-LAMP had 98% (n = 41/42) concordance and negative NP swab samples assayed by fluorescent RT-LAMP had 87% (n = 59/68) concordance for the same samples. …”
Publicado 2021
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1612A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testingpor Kellner, Max J., Ross, James J., Schnabl, Jakob, Dekens, Marcus P. S., Matl, Martin, Heinen, Robert, Grishkovskaya, Irina, Bauer, Benedikt, Stadlmann, Johannes, Menéndez-Arias, Luis, Straw, Andrew D., Fritsche-Polanz, Robert, Traugott, Marianna, Seitz, Tamara, Zoufaly, Alexander, Födinger, Manuela, Wenisch, Christoph, Zuber, Johannes, Pauli, Andrea, Brennecke, Julius“…Based on this assay, we have increased sensitivity and scalability by adding a nucleic acid enrichment step (Bead-LAMP), developed a version for home testing (HomeDip-LAMP), and identified open-source RT-LAMP enzymes that can be produced in any molecular biology laboratory. …”
Publicado 2022
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1613por Tavakoli-Koopaei, Reyhaneh, Javadi-Zarnaghi, Fatemeh, Aboutalebian, Shima, Mirhendi, Hossein“…Isothermal amplification methods such as loop-mediated-isothermal amplification (LAMP) are the method of choice in these cases. Reverse transcription steps are efficiently coupled to LAMP for the detection of pathogens with genomic RNAs such as SARS-CoV-2. …”
Publicado 2023
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1614“…The MTF1 gene was isolated from synovial fluids using an FTA card and amplified using the LAMP method at 65 °C for 35 min. A test part of the phenolphthalein-soaked swab was decolorized in the presence of the MTF1 gene due to the pH change after the LAMP, but the color remained pink in the absence of the MTF1 gene. …”
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1615“…Six specific primers were designed by targeting the PCMV DNA polymerase (DPOL) DNA. The LAMP reaction was optimized in a water bath. The sensitivity and specificity of LAMP and polymerase chain reaction (PCR) were compared. …”
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1616por Li, Jie, Wang, Peiyuan, Zhang, Aiguo, Zhang, Ping, Alsarakibi, Muhamd, Li, Guoqing“…The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. …”
Publicado 2013
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1617por Xie, Liji, Xie, Zhixun, Zhao, Guangyuan, Liu, Jiabo, Pang, Yaoshan, Deng, Xianwen, Xie, Zhiqin, Fan, Qing, Luo, Sisi“…RESULTS: The LAMP assay reaction was conducted in a 62°C water bath condition for 50 min. …”
Publicado 2014
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1618“…In this study, a group of loop-mediated isothermal amplification (LAMP) primers was designed to detect A. rabiei in chickpea plants and seeds via a LAMP method and a conventional PCR method based on an internal transcribed spacer (ITS) sequence analysis of A. rabiei. …”
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1619por Xu, Yuancong, Xiang, Wenjin, Wang, Qin, Cheng, Nan, Zhang, Li, Huang, Kunlun, Xu, Wentao“…Then, an LFD strategy was used for specific signal recognition through an immunoassay. Meanwhile, LFD-LAMP was compared to LAMP and real-time LAMP, the results showed consistent high specificity and sensitivity but in a more convenient and easy-to-use system. …”
Publicado 2017
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1620por Phillips, Elizabeth A., Moehling, Taylor J., Bhadra, Sanchita, Ellington, Andrew D., Linnes, Jacqueline C.“…We find that combining strand displacement with LAMP (SD-LAMP) yields LFIA test band intensities that can be unequivocally interpreted by human subjects without additional instrumentation, thereby alleviating the need for a portable reader’s analysis. …”
Publicado 2018
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