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1681por Qiu, Xiaohuo, Li, Tian, Zhang, Guorui, Cao, Jingjing, Jin, Yulan, Xing, Gang, Liao, Min, Zhou, Jiyong“…RESULTS: Herein, a LAMP method is developed to detect the genotypes of PCV2. …”
Publicado 2012
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1682por Hase, Katsunori, Fujiwara, Yuuki, Kikuchi, Hisae, Aizawa, Shu, Hakuno, Fumihiko, Takahashi, Shin-Ichiro, Wada, Keiji, Kabuta, Tomohiro“…GGGGGG or d(GGGG) sequences are essential for the interaction between poly-G/dG and LAMP2C. In addition to poly-G/dG, G/dG-rich sequences, such as a repeated GGGGCC sequence, interacted with the cytosolic region of LAMP2C. …”
Publicado 2015
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1683por Cuadros, Juan, Pérez-Tanoira, Ramón, Prieto-Pérez, Laura, Martin-Martin, Ines, Berzosa, Pedro, González, Vicenta, Tisiano, Gebre, Balcha, Seble, Ramos, José Manuel, Górgolas, Miguel“…A new rapid molecular malaria diagnostic test called Loop-mediated isothermal DNA amplification (LAMP) has been recently validated in clinical trials showing exceptional sensitivity and specificity features. …”
Publicado 2015
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1684por Yao, Xiefeng, Li, Pingfang, Xu, Jinghua, Zhang, Man, Ren, Runsheng, Liu, Guang, Yang, Xingping“…The LAMP assay could be accomplished in about 45 min, with the results visible to the naked eye. …”
Publicado 2016
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1685“…The lowest sheet resistance obtained in the Ag NP layer was 0.294 Ω/□, the distance between the substrate and lamp was 50-mm long, the IR lamp power was 500 W, and the sintering time was 5.4 s. …”
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1686por Chi, Ying, Ge, Yiyue, Zhao, Kangchen, Zou, Bingjie, Liu, Bin, Qi, Xian, Bian, Qian, Shi, Zhiyang, Zhu, Fengcai, Zhou, Minghao, Cui, Lunbiao, Su, Chuan“…However, for multiplex LAMP, differentiation of the amplicons derived from multiple targets is still challengeable currently. …”
Publicado 2017
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1687por Gorseta, Kristina, Glavina, Domagoj, Skrinjaric, Tomislav, Czarnecka, Beata, Nicholson, John W.“…The cure lamp emitted heat, which may enhance the flexural strength of specimens coated with light-cured varnish.…”
Publicado 2016
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1688por Song, Zonggong, Huang, Yongming, Liu, Chao, Lu, Ming, Li, Zhituo, Sun, Bei, Zhang, Weihui, Xue, Dongbo“…The function enrichment analysis showed that the functions of miR-352 and its regulatory targets lysosome-associated membrane protein 2 (LAMP2) and cathepsin L1 (CTSL1) were lysosome related. …”
Publicado 2018
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1689“…METHODS: A one-step single tube accelerated quantitative RT-LAMP assay was evaluated by targeting the Env gene of WNV. …”
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1690por Barnes, Lucien, Heithoff, Douglas M., Mahan, Scott P., Fox, Gary N., Zambrano, Andrea, Choe, Jane, Fitzgibbons, Lynn N., Marth, Jamey D., Fried, Jeffrey C., Soh, H. Tom, Mahan, Michael J.“…METHODS: A smartphone-based real-time loop-mediated isothermal amplification (smaRT-LAMP) system was developed for pathogen ID in urinary sepsis patients. …”
Publicado 2018
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1691por Lai, Dingwang, Zhang, Yuliang, Huang, Qixing, Yin, Guohua, Pennerman, Kayla K., Liu, Zhixin, Guo, Anping“…Our results indicated that reverse transcription loop-mediated isothermal amplification (RT-LAMP) reactions are more efficient and sensitive than RT-PCR for RRSV detection. …”
Publicado 2018
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1692“…We evaluated whether loop-mediated isothermal amplification (LAMP) for screening malaria parasites could reduce the workload in the diagnosis of malaria. …”
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1693por Kitamura, Masashi, Kazato, Akira, Yamamuro, Tadashi, Ando, Hirokazu, Sasaki, Yohei, Suzuki, Ryuichiro, Shirataki, Yoshiaki“…Using the LAMP primers, the LAMP assay included an initiation reaction of 10 min followed by amplification for 20 min at the isothermal reaction temperature of 65 °C. …”
Publicado 2019
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1694“…This research first establishment a loop-mediated isothermal amplification (LAMP) assay in rapidly identifying O. sinensis, upon the basis of the complete internal transcribed spacers (ITS) gene. …”
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1695“…Patients were examined by a clinician and graded for SVP with a slit lamp and 78 Dioptre lens. Videos were taken with a smartphone ophthalmoscope and graded by two separate clinicians blinded to the slit lamp findings. …”
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1696por Zhang, Yubao, Xie, Zhongkui, Fletcher, John D, Wang, Yajun, Wang, Ruoyu, Guo, Zhihong, He, Yuhui“…RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. …”
Publicado 2020
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1697por Korbut, Anton I., Taskaeva, Iuliia S., Bgatova, Nataliya P., Muraleva, Natalia A., Orlov, Nikolai B., Dashkin, Maksim V., Khotskina, Anna S., Zavyalov, Evgenii L., Konenkov, Vladimir I., Klein, Thomas, Klimontov, Vadim V.“…LC3B and LAMP-1, autophagy markers, and caspase-3 and Bcl-2, apoptotic markers, were evaluated in renal cortex by western blot. …”
Publicado 2020
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1698por Ganguli, A., Mostafa, A., Berger, J., Aydin, M., Sun, F., Valera, E., Cunningham, B. T., King, W. P., Bashir, R.“…As assay details and primer sequences become widely known, many laboratories could perform diagnostic tests using methods such as RT-PCR or isothermal RT-LAMP amplification. A key advantage of RT-LAMP based approaches compared to RT-PCR is that RT-LAMP is known to be robust in detecting targets from unprocessed samples. …”
Publicado 2020
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1699por Yu, Shao-shuai, Che, Hai-yan, Wang, Sheng-jie, Lin, Cai-li, Lin, Ming-xing, Song, Wei-wei, Tang, Qing-hua, Yan, Wei, Qin, Wei-quan“…The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loop-mediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64oC for 40 min. …”
Publicado 2020
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1700“…In our preliminary study, we discovered that leptin downregulated cathepsin A, which is responsible for lysosomal-associated membrane protein 2a (LAMP2a) degradation. LAMP2a is a rate-limiting factor in chaperone-mediated autophagy and is highly active in malignant cancers. …”
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