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1761por Zhang, Xuzhi, Yang, Qianqian, Zhang, Qingli, Jiang, Xiaoyu, Wang, Xiaochun, Li, Yang, Zhao, Jun, Qu, Keming“…The nirS gene of P. aeruginosa PAO1 in spiked seawater samples could be detected with both DNA-template based LAMP assay and cell-template based LAMP assay, demonstrating the practicality of in-field use.…”
Publicado 2020
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1762por Shimizu, Eisuke, Yazu, Hiroyuki, Aketa, Naohiko, Yokoiwa, Ryota, Sato, Shinri, Katayama, Taiichiro, Hanyuda, Akiko, Sato, Yasunori, Ogawa, Yoko, Tsubota, Kazuo“…PURPOSE: This study aimed to demonstrate the efficacy of a “Smart Eye Camera (SEC)” in comparison with the efficacy of the conventional slit-lamp microscope by evaluating their diagnostic functionality for dry eye disease (DED) in clinical cases. …”
Publicado 2021
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1763por Carvalho, Joana, Garrido-Maestu, Alejandro, Azinheiro, Sarah, Fuciños, Pablo, Barros-Velázquez, Jorge, De Miguel, Ramón J., Gros, Verónica, Prado, Marta“…Regarding sensitivity, both qPCR approaches were more sensitive than qLAMP by one order of magnitude, however the qLAMP method proved to be as specific and much faster being performed in just 9 min versus 23 and 29 min for the qPCR methods based on hydrolysis probe and intercalating dye respectively.…”
Publicado 2021
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1764por Chen, Nianzhen, Si, Yuying, Li, Gen, Zong, Ming, Zhang, Wenyan, Ye, Yangqin, Fan, Lieying“…We also evaluated the result of our RT-LAMP assay on 638 nasopharyngeal swab specimens with the commercial RT-PCR by Cohen’s Kappa. …”
Publicado 2021
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1765por Promlek, Thanyarat, Thanunchai, Maytawan, Phumisantiphong, Uraporn, Hansirisathit, Tonsan, Phuttanu, Chayanit, Dongphooyao, Sunisa, Thongsopa, Wipawee, Nuchnoi, Pornlada“…The sensitivity and specificity of RT-LAMP were determined in comparison with reverse transcriptase–polymerase chain reaction (RT-PCR). …”
Publicado 2022
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1766por Mo, Ying-Qian, Nakamura, Hiroyuki, Tanaka, Tsutomu, Odani, Toshio, Perez, Paola, Ji, Youngmi, French, Benjamin N., Pranzatelli, Thomas J.F., Michael, Drew G., Yin, Hongen, Chow, Susan S., Khalaj, Maryam, Afione, Sandra A., Zheng, Changyu, Oliveira, Fabiola Reis, Motta, Ana Carolina F., Ribeiro-Silva, Alfredo, Rocha, Eduardo M., Nguyen, Cuong Q., Noguchi, Masayuki, Atsumi, Tatsuya, Warner, Blake M., Chiorini, John A.“…BMP6 expression could be experimentally induced in mice by overexpression of LAMP3, which developed an SS-like phenotype. The newly identified LAMP3/HSP70/BMP6 axis provided an etiological model for SS gland dysfunction and autoimmunity.…”
Publicado 2022
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1767Highly Sensitive Detection of Human Pluripotent Stem Cells by Loop-Mediated Isothermal Amplificationpor Yasui, Ryota, Matsui, Atsuka, Sekine, Keisuke, Okamoto, Satoshi, Taniguchi, Hideki“…LAMP could readily assay microgram order of input sample per test and detected an equivalent model of 0.00002% hiPSC contamination in a simple one-pot reaction. …”
Publicado 2022
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1768por Yoshii, Toshitaka, Morishita, Shingo, Egawa, Satoru, Sakai, Kenichiro, Kusano, Kazuo, Tsutsui, Shunji, Hirai, Takashi, Matsukura, Yu, Wada, Kanichiro, Katsumi, Keiichi, Koda, Masao, Kimura, Atsushi, Furuya, Takeo, Maki, Satoshi, Nagoshi, Narihito, Nishida, Norihiro, Nagamoto, Yukitaka, Oshima, Yasushi, Ando, Kei, Nakashima, Hiroaki, Takahata, Masahiko, Mori, Kanji, Nakajima, Hideaki, Murata, Kazuma, Miyagi, Masayuki, Kaito, Takashi, Yamada, Kei, Banno, Tomohiro, Kato, Satoshi, Ohba, Tetsuro, Inami, Satoshi, Fujibayashi, Shunsuke, Katoh, Hiroyuki, Kanno, Haruo, Taneichi, Hiroshi, Imagama, Shiro, Kawaguchi, Yoshiharu, Takeshita, Katsushi, Matsumoto, Morio, Yamazaki, Masashi, Okawa, Atsushi“…Herein, we prospectively investigated the surgical outcomes of anterior cervical decompression with fusion (ADF) and laminoplasty (LAMP) with cervical OPLL. Three hundred patients were included in this study (ADF: n = 89; LAMP: n = 211 patients), and propensity score matching yielded 67 pairs of patients with ADF and LAMP, in which clinical outcomes were compared. …”
Publicado 2022
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1769por Wang, Xiang, Wang, Lai-Fa, Cao, Ye-Fan, Yuan, Yan-Zhi, Hu, Jian, Chen, Zu-Hai, Zhu, Fei, Wang, Xi-Zhuo“…Compared with the single LAMP method, the LAMP-CRISPR/Cas12a assay uses species-specific fluorescence cleavage to detect target amplicons. …”
Publicado 2022
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1770por Kim, Eun Jin, Lee, Jiwon, Yoon, Youngbae, Lee, Donghyun, Baek, Yeongjun, Takano, Chika, Sakai, Jun, Iijima, Takahiro, Kanamori, Dai, Gardner, Humphrey, McLaughlin, Robert E., Kilgore, Paul E., Nakamura, Akihiro, Ogihara, Takashi, Hayakawa, Satoshi, Hoshino, Tomonori, Kim, Dong Wook, Seki, Mitsuko“…We developed loop-mediated isothermal amplification (LAMP)-based assays for four β-lactamase genes (bla (KPC), bla (NDM-1), bla (IMP-1) group, and bla (VIM)). …”
Publicado 2023
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1771por Rawling, David, Nie, Wensheng, Remmel, Melissa, Eshoo, Mark, Romanowsky, Jonathan, Liesenfeld, Oliver, Sweeney, Timothy“…Loop-mediated isothermal amplification (LAMP) represents a rapid amplification solution with the potential to meet these needs. …”
Publicado 2018
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1772por Zhu, Xiong, Wang, Xiaoxia, Han, Limei, Chen, Ting, Wang, Licheng, Li, Huan, Li, Sha, He, Lvfen, Fu, Xiaoying, Chen, Shaojin, Xing, Mei, Chen, Hai, Wang, Yi“…Hence, we devised a multiplex reverse transcription loop-mediated isothermal amplification (mRT-LAMP) coupled with a nanoparticle-based lateral flow biosensor (LFB) assay (mRT-LAMP-LFB) for diagnosing COVID-19. …”
Publicado 2020
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1773por Gao, Kankan, Deng, Qiulian, Huang, Lianfen, Chang, Chien-Yi, Zhong, Huamin, Xie, Yongqiang, Guan, Xiaoshan, Liu, Haiying“…Our data suggested that the performance of LAMP was highly comparable to the standard Lim broth-enriched subculture and LAMP is considered as an alternative for fast and accurate GBS screening.…”
Publicado 2021
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1774por Blackmore, Claire, Hall, Gareth W, Allsopp, Rebecca C, Hansell, Anna L, Cowley, Caroline M, Barber, Ruth C, Holmes, Christopher W, Tobin, Martin D, Shaw, Jacqui A, Brunskill, Nigel J, Baker, Philip N“…The programme used RT-LAMP testing to identify asymptomatic cases within the population. …”
Publicado 2021
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1775por Dassanayake, Rohana P., Clawson, Michael L., Tatum, Fred M., Briggs, Robert E., Kaplan, Bryan S., Casas, Eduardo“…The overall detection sensitivity and specificity of the newly developed colorimetric LAMP assay for each genotype were 100%. The limits of detection of two LAMP assays were 1–100 target gene copies per reaction. …”
Publicado 2023
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1776por Sakaki, Kyohei, Sakai, Kenichiro, Arai, Yoshiyasu, Torigoe, Ichiro, Tomori, Masaki, Hirai, Takashi, Onuma, Hiroaki, Kobayashi, Yutaka, Okawa, Atsushi, Yoshii, Toshitaka“…A series of 55 cases with C-OPLL: 13 anterior decompression with fusion (ADF), 16 posterior decompression with fusion (PDF), and 26 laminoplasty (LAMP), and a series of 123 cases with CSM: 61 ADF, 5 PDF, and 57 LAMP, were included. …”
Publicado 2023
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1777“…The phagomagnetic-based approach succeeded in the selective bacterial capture and ensuing lysis, triggering Listeria DNA leakage, which was efficiently LAMP amplified. Methylene blue-based electrochemical detection of LAMP amplicons was accomplished in 20 min with remarkable analytical sensitivity (1 CFU mL(−1)). …”
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1778“…These results offered reasons for further investigations into the functions of IGFBP7 and LAMP2 in MS and NMOSD.…”
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1779por Ocwieja, Karen E., Sherrill-Mix, Scott, Liu, Changchun, Song, Jinzhao, Bau, Haim, Bushman, Frederic D.“…Detection based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is particularly useful for this, because when combined with fluorescence-based DNA detection, RT-LAMP can be implemented with minimal equipment and expense. …”
Publicado 2015
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1780por Bhadra, Sanchita, Jiang, Yu Sherry, Kumar, Mia R., Johnson, Reed F., Hensley, Lisa E., Ellington, Andrew D.“…Additionally we applied one-step strand displacement probes (OSD) for real-time sequence-specific verification of LAMP amplicons. Asymmetric amplification effected by incorporating a single loop primer in each assay accelerated the time-to-result of the OSD-RT-LAMP assays. …”
Publicado 2015
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