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1781por Bakhtiari, Somaye, Alvandi, Amirhooshang, Pajavand, Hamid, Navabi, Jafar, Najafi, Farid, Abiri, Ramin“…OBJECTIVES: The aim of this study was to set up and evaluate a diagnostic value of loop- mediated isothermal amplification (LAMP) for detecting H. pylori. PATIENTS AND METHODS: The analytical sensitivity values (limit of detection) of LAMP and polymerase chain reaction (PCR) were determined using serial dilutions of H. pylori DNA. …”
Publicado 2016
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1782“…Amplification was carried out using Rotorgene 6000 and the portable ESE Quant amplification unit capable of real-time data output. The stem LAMP formats indicated shorter time to results (~8 min), were 10–100-fold more sensitive, and indicated higher diagnostic sensitivity and accuracy compared to the standard LAMP test. …”
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1783“…Free-spheroids showed the initial (LC3-autophagosomes) but not the late (Lamp1/Lamp2-lysosomes) components of autophagy (incomplete autophagy), preparing their autophagosomes for an external phagocytosis (accumulation of phosphatidylserine). …”
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1784por Yap, Chan Choo, Digilio, Laura, McMahon, Lloyd P., Garcia, A. Denise R., Winckler, Bettina“…Unlike in fibroblasts, we found that the majority of dendritic Rab7 late endosomes (LEs) do not contain LAMP1 and that a large proportion of LAMP1 compartments do not contain CatB/D. …”
Publicado 2018
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1785por Sugie, Kazuma, Komaki, Hirofumi, Eura, Nobuyuki, Shiota, Tomo, Onoue, Kenji, Tsukaguchi, Hiroyasu, Minami, Narihiro, Ogawa, Megumu, Kiriyama, Takao, Kataoka, Hiroshi, Saito, Yoshihiko, Nonaka, Ikuya, Nishino, Ichizo“…Male patients showed completely absent LAMP-2 expression in muscle. In contrast, female patients showed decreased LAMP-2 expression, which is suggested to reflect LAMP-2 haploinsufficiency due to a heterozygous null mutation. …”
Publicado 2018
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1786por Kühbacher, Andreas, Novy, Karel, Quereda, Juan J, Sachse, Martin, Moya-Nilges, Maryse, Wollscheid, Bernd, Cossart, Pascale, Pizarro-Cerdá, Javier“…Our data further shows that the lysosomal-associated membrane proteins LAMP-1 and LAMP-2 are translocated to the cellular surface and those LLO-induced Ca(2+) fluxes are required to trigger the surface relocalization of LAMP-1. …”
Publicado 2018
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1787por Stepp, Mary Ann, Pal-Ghosh, Sonali, Tadvalkar, Gauri, Williams, Alexa R., Pflugfelder, Stephen C., de Paiva, Cintia S.“…Quantitative PCR was performed to quantify expression of beclin 1, LC3, Lamp-1, Lamp-2, CXCL-1, BDNF, NTN1, DCC, Unc5b1, Efna4, Efna5, Rgma, and p21 in corneal epithelial mRNA. …”
Publicado 2018
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1788“…All 12 non-ZIKA respiratory pathogens tested were negative for LAMP detection, indicating the high specificity of the primers for ZIKV. …”
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1789por Hongjaisee, Sayamon, Doungjinda, Natteewan, Khamduang, Woottichai, Carraway, Tanawan Samleerat, Wipasa, Jiraprapa, Debes, Jose D., Supparatpinyo, Khuanchai“…OBJECTIVES: The aim was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of hepatitis C virus (HCV) in a single closed tube. …”
Publicado 2020
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1790por Szpor, Joanna, Streb, Joanna, Glajcar, Anna, Frączek, Paulina, Winiarska, Aleksandra, Tyrak, Katarzyna E., Basta, Paweł, Okoń, Krzysztof, Jach, Robert, Hodorowicz-Zaniewska, Diana“…This study aimed to investigate the density of DCs expressing CD1a, CD83, CD123, DC-LAMP3 (CD208) and DC-SIGN (CD209) in breast cancer. …”
Publicado 2021
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1791por Oscorbin, Igor P., Shevelev, Georgiy Yu., Pronyaeva, Ksenia A., Stepanov, Andrey A., Shamovskaya, Darya V., Mishukova, Olga V., Pyshnyi, Dmitrii V., Filipenko, Maksim L.“…Loop-mediated isothermal amplification (LAMP) is a method of nucleic acid amplification that is more stable and resistant to DNA amplification inhibitors than conventional PCR. …”
Publicado 2021
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1792por Wanjala, Bramwel W., Ateka, Elijah M., Miano, Douglas W., Fuentes, Segundo, Perez, Ana, Low, Jan W., Kreuze, Jan F.“…We developed a simple and rapid loop‐mediated isothermal amplification (LAMP) assay for the detection of SPFMV, SPCSV and begomoviruses related to sweet potato leaf curl virus (SPLCV). …”
Publicado 2021
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1793por Xie, Xiaomei, Li, Bu, Fan, Yuguang, Duan, Renhe, Gao, Chonghua, Zheng, Yuan, Tian, Enjing“…This study aimed to establish a rapid and visual method of G. infula identification based on loop-mediated isothermal amplification (LAMP). A set of specific LAMP primers was designed, and its specificity in G. infula was confirmed against various mushroom species, including its closely related species and other macrofungi. …”
Publicado 2022
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1794“…In this study, we developed a rotatable paper device integrating loop-mediated isothermal amplification (RT-LAMP) and a novel naked-eye readout of the RT-LAMP results using a food additive, carmoisine, for infectious pathogen detection. …”
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1795por Logeshwari, R., Gopalakrishnan, C., Kamalakannan, A., Ramalingam, J., Saraswathi, R.“…Loop-mediated isothermal amplification (LAMP) is ideal for field-level diagnosis since it offers quick, high-specific amplification of target template sequences at a single temperature. …”
Publicado 2022
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1796por Neshani, Alireza, Zare, Hosna, Sadeghian, Hamid, Safdari, Hadi, Riahi-Zanjani, Bamdad, Aryan, Ehsan“…Interestingly, EBT-LAMP showed the lowest potential for subjective error in reading the results. …”
Publicado 2023
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1797“…In this study, a rapid and cheap method of detecting S. enterica DNA was developed by employing the Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification (QUASR LAMP) platform. QUASR LAMP provides a closed-tube, target-specific endpoint detection of pathogens, wherein results can be analyzed visually through an LED transilluminator and verified through agarose gel electrophoresis. …”
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1798“…The development of an in-line digestion system based on simple UV lamp is reported. The effect of photolysis on the preconcentration of copper was investigated using an in-line Chelex-l00 ion-exchange column linked to an atomic absorption spectrometer. …”
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1799“…In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID(50))/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID(50) per one RT-LAMP reaction. …”
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1800por OKUWA, Yuka, MIYAMATO-HAYASHI, Michiko, TANAKA, Takaichi, HAYAKAWA, Yuji, INOSHIMA, Yasuo“…Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. …”
Publicado 2016
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