“…Construction of a multiplexed gRNA library based on single guide RNA (sgRNA) performance enabled simultaneous gene repression, yielding a 3 to 4.5-fold increase in isoprenol titer (1.82 ± 0.19 g/L) on M9-MOPS minimal medium. We then scaled the best performing CRISPRi strain to 2-L fed-batch cultivation and demonstrated translatable titer improvements, ultimately obtaining 12.4 ± 1.3 g/L isoprenol. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…For slow freezing, Dulbecco phosphate buffered saline based 10% glicerol solution was used. For vitrification, G-MOPS™ based solution supplemented with 16% ethylene glycol, 16% propylene glycol, Ficoll (10 mg/ml) and sucrose (0.65 mol/l) was used. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…A CoV RNA sequence similar to Chaerephon bat coronavirus/Kenya/KY22/2006 (BtKY22) was found in a Chaerephon cf. pumilus and a Mops sp. fecal swab, while a CoV RNA sequence similar to the two almost identical Kenya bat coronaviruses BtKY55 and BtKY56 (BtKY55/56) was detected in an Epomops buettikoferi oral swab. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…When compared to the recently available FREEC and cn.MOPS approaches, our findings were more concordant with putative hits from the highest quality array data for the 7G8 and GB4 isolates. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In addition, the addition of 40 mM MOPS to the growth medium increased the maximal growth yield of C. bescii by approximately twofold. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Predictors of voluntary early retirement were persistent shoulder pain HR: 1.40 (95 % CI 1.16 to 1.67) and floor mopping for more than 10 h per week HR: 1.20 (95 % CI 1.03 to 1.40). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We used the DELLY software to identify deletions and insertions, cn.MOPS to identify copy-number variants (CNVs). Furthermore, we annotated these structural variations using different bioinformatics tools, such as gene ontology, cattle quantitative trait locus (QTL) database and ingenuity pathway analysis (IPA). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In contrast, C. auris biofilms formed in RPMI-MOPS were approximately half the density of the C. albicans biofilms. …”
Enlace del recurso
Enlace del recurso

“…This response to the last outbreak has to be evaluated independently to ensure effective mopping and surveillance to stop further outbreak all over the country including the holy province Karbala. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To estimate biofilm production, echinocandin resistant (n = 2), borderline resistant (n = 5), and susceptible isolates (n = 3) were seeded at the cell concentration of 1 × 10⁶ cells/mL in RPMI-1640 with 0.165 M MOPS in polystyrene 96-well microtiter plates and incubated for 24 and 48 h at 37°C. …”
Enlace del recurso
Enlace del recurso

“…The incorporation of glass beads (ø 3 mm) in MTPs not only improved the process performance in terms of improved oxygen transfer improving secondary metabolite production, but also helped to transform morphology from pellet to disperse, resulting in enhanced reproducibility. Addition of MOPS into the medium resulted in pH maintenance above 6.50, a crucial parameter towards reproducibility. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Novel CalVs were detected in Rousettus aegyptiacus and Mops condylurus while novel Rotavirus-A-related viruses were detected in Taphozous bats and R. aegyptiacus. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Migration of surgical sponge (gauze, mops) into the urinary bladder is uncommon when compared to other abdominal and pelvic viscus. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Cleaning activities highlighted included mopping and disinfecting high-touch surfaces, bathrooms, and floors. …”
Enlace del recurso
Enlace del recurso

“…Abbreviations used: NO: Nitric oxide, PBMC: Peripheral blood mononuclear cells, CE: Crude extract, AF: Ethyl-acetate fraction, BF: n-butanol fraction, HF: Hexane fraction, WF: Water fraction, MIC: Minimum inhibitory concentration, MBC: Minimum bactericidal concentration, ATCC: American Type Culture Collection, CFU: Colony-forming units, BHI: Brain heart infusion, RPMI: Roswell Park Memorial Institute, MOPS: 3-(N-morpholino)propanesulfonic acid, DMEM: Dulbecco's modified Eagle's médium, LPS: Lipopolysacharide, MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide, OD: Optical density, AC: Acteoside,…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The experiments were performed with 0.2–0.5 mM precipitate-Fe(III) using 10 mM Na-ascorbate as reductant, 5 mM bipyridine as Fe(II)-complexing ligand, and 10 mM MOPS/5 mM NaOH as pH 7.0 buffer. Times required for the dissolution of half of the precipitate (t(50%)) ranged from 1.5 to 39 h; spanning a factor 25 range. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We used GenomeSTRiP and cn.MOPS to identify copy number variant regions (CNVRs). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…As a result, C. neoformans was grown in either YPD or YNB, which were either left unbuffered or buffered to pH 7 with MOPS. These cells were then labeled with cell wall-specific fluorescent probes to determine the amounts of various cell wall components. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To examine the impact of growth phase on the dynamics of the transcriptome, O157:H7 Sakai strain was cultured in MOPS minimal media (0.1% glucose), RNA harvested at 10 time points from early exponential to full stationary phase, and relative gene expression was measured by co-hybridization on high-density DNA microarrays. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we present a comparison of transcriptional profiles measured using densely tiled oligonucleotide arrays for two phytopathogens, Dickeya dadantii 3937 and Pectobacterium atrosepticum SCRI1043, grown to mid-log phase in MOPS minimal medium (0.1% glucose) with and without O(2). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso