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  1. 2701
    “…OBJECTIVE: This study aimed to investigate whether the research output during the second decade after the release of the NHI claims database continues growing, to explore how the emergence of open access mega journals (OAMJs) and lawsuit against the use of this database affect the research topics and publication volume and to discuss the underlying reasons. …”
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    Two Decades of Research Using Taiwan’s National Health Insurance Claims Data: Bibliometric and Text Mining Analysis on PubMed
  2. 2702
    “…Phylogenetic analyses were carried out with MEGA 7.0 software. Graphs were drawn with GraphPad Prism 8.0 software. …”
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    Natural Wolbachia infection in field-collected Anopheles and other mosquito species from Malaysia
  3. 2703
    “…BACKGROUND & AIMS: Given ongoing challenges in non-invasive non-alcoholic liver disease (NAFLD) diagnosis, we sought to validate an ALT-based NAFLD phenotype using measures readily available in electronic health records (EHRs) and population-based studies by leveraging the clinical and genetic data in the Million Veteran Program (MVP), a multi-ethnic mega-biobank of US Veterans. METHODS: MVP participants with alanine aminotransferases (ALT) >40 units/L for men and >30 units/L for women without other causes of liver disease were compared to controls with normal ALT. …”
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    Validating a non-invasive, ALT-based non-alcoholic fatty liver phenotype in the million veteran program
  4. 2704
    “…Gene sequences were analysed using BioEdit, MEGA7, and DnaSP programs. Mutations and gene amplifications were compared with P. falciparum and Plasmodium vivax orthologous genes. …”
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    Genetic analysis of the orthologous crt and mdr1 genes in Plasmodium malariae from Thailand and Myanmar
  5. 2705
    “…Integrating trials into mega-cohorts should improve health and well-being by generating faster, larger-scale evidence on a longer and/or broader horizon than previously possible. …”
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    Integrating trials into a whole-population cohort of children and parents: statement of intent (trials) for the Generation Victoria (GenV) cohort
  6. 2706
    “…Genotyping was done using the Illumina Multi-Ethnic Genotyping Array (MEGA) and imputation was done using the African Genome Resources Haplotype Reference Panel. …”
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    Genome-wide association study of prevalent and persistent cervical high-risk human papillomavirus (HPV) infection
  7. 2707
    “…Phylogenetic and recombination analysis of SARS-CoV-2 genome with nearest animal and human coronaviruses were analyzed using MEGA X and SimPlot version 3.5.1 software respectively. …”
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    TSP-based PCR for rapid identification of L and S type strains of SARS-CoV-2
  8. 2708
    “…The PCR products were sequenced and analyzed using MEGA 7.0 software. RESULTS: A total of 503 (97.7%) H. pylori strains were cagA-positive and 1,587 EPIYA motifs were identified, including 12 types of EPIYA or EPIYA-like sequences. …”
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    Diversity of 3′ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population
  9. 2709
    “…Detailed sequence comparison, genetic heterogeneity analyses and maximum-likelihood phylogenetic tree construction were performed with BioEdit Sequence Alignment Editor and MEGA X software. Data for cytology tests and histological diagnoses were obtained from our Taizhou Area Study with longitudinal follow-up for at least 5 years. …”
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    Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
  10. 2710
    “…Phylogenetic trees were reconstructed using the neighbor-joining method with the Kimura 2-parameter model on MEGA X version 10.2.2. RESULTS: A total of 46 anopheline specimens were used for the phylogenetic analysis. …”
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    Updated distribution of anopheline mosquitoes in Hokkaido, Japan, and the first evidence of Anopheles belenrae in Japan
  11. 2711
    “…All positive RT-PCR amplification products were sequenced and the obtained sequences analyzed by MEGA (version 7.0). Demographic information and clinical manifestation data were also analyzed. …”
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    Molecular and epidemiological characterization of human adenoviruses infection among children with acute diarrhea in Shandong Province, China
  12. 2712
    “…The sequences were analysed using the MEGA-X tool, and a phylogenetic tree was generated. …”
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    Roles of two distinct alphasatellites modulating geminivirus pathogenesis
  13. 2713
    “…This paper, therefore, analyzes transcription start sites, promoter regions, motifs, transcription factors, and CpG islands in TetR family transcriptional regulatory (TFTR) genes of M. colombiense CECT 3035 using neural network promoter prediction, MEME, TOMTOM algorithms, and evolutionary analysis with the help of MEGA-X. RESULTS: The analysis of 22 protein coding TFTR genes of M. colombiense CECT 3035 showed that 86.36% and 13.64% of the gene sequences had one and two TSSs, respectively. …”
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    In silico analysis of promoter regions to identify regulatory elements in TetR family transcriptional regulatory genes of Mycobacterium colombiense CECT 3035
  14. 2714
    “…The power and simplicity of the method is demonstrated by two filter samples from heavily polluted mega cities. The samples were collected in Tehran (Iran) and Hangzhou (China) in February 2018. …”
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    Particle characterization and quantification of organic and inorganic compounds from Chinese and Iranian aerosol filter samples using scanning laser desorption/ionization mass spectrometry
  15. 2715
    “…The downstream population genetic analyses were performed using MEGA4, DnaSP, Arlequin v3.5 and Network.5 resources. …”
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    Population genetic analyses inferred a limited genetic diversity across the pvama-1 DI domain among Plasmodium vivax isolates from Khyber Pakhtunkhwa regions of Pakistan
  16. 2716
    “…BACKGROUND: By entering the era of mega-scale genomics, we are facing many computational issues with standard genomic evaluation models due to their dense data structure and cubic computational complexity. …”
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    Optimisation of the core subset for the APY approximation of genomic relationships
  17. 2717
    “…Air quality improved significantly during the middle phases of the lockdown (April and May), especially in small metropolitan cities like Amman, Beirut, and Jeddah, while it was less significant in “mega” cities like Cairo, Tehran, and Istanbul. For example, the concentrations of NO(2) in Amman, Beirut, and Jeddah decreased by −56.6%, −43.4%, and −32.3%, respectively, during April 2020, compared to April 2019. …”
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    The impact of COVID-19 lockdowns on surface urban heat island changes and air-quality improvements across 21 major cities in the Middle East()()
  18. 2718
    “…For HAdV typing, phylogenetic trees were built by MEGA X. Then clinical data of HAdV positive cases were collected. …”
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    The changed endemic pattern of human adenovirus from species B to C among pediatric patients under the pressure of non-pharmaceutical interventions against COVID-19 in Beijing, China
  19. 2719
    “…Nucleotide similarity was calculated using MEGA to construct a phylogenetic tree and DNASTAR. …”
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    A new PEDV strain CH/HLJJS/2022 can challenge current detection methods and vaccines
  20. 2720
    “…The polymerase chain reaction (PCR) products were then sequenced, and data were analyzed using the MEGA version 7.0, DNA SP version 6.0, and NTSYS-pc version 2.11 software. …”
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    Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences
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