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  1. 2741
    “…METHODS: PEDV was detected by reverse transcription-polymerase chain reaction (RT-PCR), and S1 and ORF3 sequences were processed by the Clustal W method via DNAMAN 8 software, and phylogenetic trees were constructed by the neighbor-joining method using MEGA 6 software. RESULTS: The prevalence of PEDV was 92.25% and was detected in 119 of 129 samples, with 94.03% (63 of 67) of pig farms harbouring the disease. …”
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    Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene
  2. 2742
    “…Phylogenetic analyses were conducted using MEGA (ver. 6.06). RESULTS: In total, 196 (26.3%) specimens were identified as NoV-positive. …”
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    Clinical and molecular analyses of norovirus-associated sporadic acute gastroenteritis: the emergence of GII.17 over GII.4, Huzhou, China, 2015
  3. 2743
    “…DNA barcoding gap was realized based on the Kimura two-parameter distance model (K2P) in TaxonDNA and MEGA. PRINCIPAL FINDINGS: Based on the K2P distance, significant divergences between the inter- and intra-specific genetic distances were observed, while the presence of a DNA barcoding gap was obvious. …”
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    Feasibility of nuclear ribosomal region ITS1 over ITS2 in barcoding taxonomically challenging genera of subtribe Cassiinae (Fabaceae)
  4. 2744
    “…HIV-1 subtyping was computed in MEGA6. Eighty seven (93.5%) of the eligible samples were successfully sequenced. …”
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    Surveillance of HIV-1 pol transmitted drug resistance in acutely and recently infected antiretroviral drug-naïve persons in rural western Kenya
  5. 2745
    “…In this paper, we use RAINBIO – one of the largest mega-databases of tropical African vascular plant species distributions ever compiled – to address questions about plant and growth form diversity across tropical Africa. …”
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    Exploring the floristic diversity of tropical Africa
  6. 2746
    “…To investigate the genetic diversity of PvMSP-1, the 95 nucleotide sequences of the PvMSP-1 icb5-6 fragment were genotyped and analyzed using DnaSP v5, MEGA software. RESULTS: The 95 P. vivax isolates collected from Zhejiang province were either indigenous cases or imported cases from different regions around the world. …”
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    Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
  7. 2747
    “…METHODS: Through database searching and citation tracking, we identified 7 systematic reviews of systematic reviews, 32 systematic reviews (including 17 meta-analyses and 8 qualitative reviews); six mega-trials and over 60 additional relevant empirical studies and commentaries. …”
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    Understanding heart failure; explaining telehealth – a hermeneutic systematic review
  8. 2748
    “…No episode of ileus or mega colon was documented. Most common treatment regimen were metronidazole 500 mg per orally every 8 hourly (65%) and vancomycin 125 mg per orally four times a day (58.8%). …”
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    Clostridium difficile Infection in Hematopoietic Stem Cell Transplant Patients: A Single-center Experience
  9. 2749
    “…Phylogenetic analysis of assembled sequences was performed in MEGA7 using the maximum likelihood method. RESULTS: For the HN gene sequence of 1715 bp was obtained for 10 of 12 patients (5 in each group). …”
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    Molecular Epidemiological Investigation of Human Parainfluenza 3 Virus Outbreak in a Pediatric Bone Marrow Transplantation Unit
  10. 2750
    “…Then, the PCR products were sequenced and analysed using BioEdit, MEGA6, and DnaSP programs. RESULTS: The average pairwise nucleotide diversities (π) of P. malariae trap, ama1, and p48/45 were 0.00169, 0.00413, and 0.00029, respectively. …”
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    Genetic diversity of three surface protein genes in Plasmodium malariae from three Asian countries
  11. 2751
    “…Polymorphic characteristics and effect of natural selection were analysed with using DNASTAR, MEGA4, and DnaSP programs. Polymorphic nature and natural selection in 459 global PfAMA-1 were also analysed. …”
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    Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates
  12. 2752
    “…A surface imaging system, Catalyst (C‐Rad), was compared with laser‐based positioning and daily mega voltage computed tomography (MVCT) setup for breast patients with nodal involvement treated by helical TomoTherapy. …”
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    Surface imaging, laser positioning or volumetric imaging for breast cancer with nodal involvement treated by helical TomoTherapy
  13. 2753
    “…Phylogenetic trees were then constructed by maximum likelihood phylogeny test (MEGA7.0), followed by analysis of nucleotide mutation and amino acid substitution. …”
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    Molecular Characterization of Dengue Virus Serotype 2 Cosmospolitan Genotype From 2015 Dengue Outbreak in Yunnan, China
  14. 2754
    “…By comparing clinical features in patients with significant CNVs against those with non-significant CNVs, a statistically significant association was found between the presence of significant CNVs and speech and language delay for those aged above 2 years and for those with facial malformations, microcephaly, congenital heart disease, fair skin, eye malformations, and mega cisterna magna. Multivariate logistic regression analysis allowed the identification of two independent significant CNV predictors, which are eye malformations and facial malformations. …”
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    Rare Copy Number Variations and Predictors in Children With Intellectual Disability and Epilepsy
  15. 2755
    “…Motif discovery was carried out using the MEME software, sequence identity calculation was done using an in-house Python script, multiple sequence alignments were performed using PROMALS3D and TCOFFEE tools, and phylogenetic tree calculations were performed using MEGA vs 7.0 tool. Possible alternative binding sites were predicted using FTMap webserver and SiteMap tool. …”
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    Aminoacyl tRNA synthetases as malarial drug targets: a comparative bioinformatics study
  16. 2756
    “…Purpose: To propose a novel method to improve the mega-voltage CT (MVCT) image quality for helical TomoTherapy while maintaining the stability on dose calculation. …”
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    A Megavoltage CT Image Enhancement Method for Image-Guided and Adaptive Helical TomoTherapy
  17. 2757
    “…The bioinformatics analyses were inferred by Maximum likelihood method and the evolutionary relatedness was deduced by MEGA X. RESULTS: Only 21 (16.8%) out of 125 isolates were hemolytic and 10 of these (47.62%) harbored the toxins encoding genes (cnf 1(+), clbA(+) and clbQ(+)). …”
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    Prevalence and pathologic effects of colibactin and cytotoxic necrotizing factor-1 (Cnf 1) in Escherichia coli: experimental and bioinformatics analyses
  18. 2758
    “…Nucleotide sequences were translated into amino acids by Mega 6.0 software. The physical/chemical properties, amino acid-conserved sequences and secondary structure of protein sequences were analysed by the Protparam server, ConSurf server and PSIPRED software. …”
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    Identification of variants and therapeutic epitopes in HPV-33/HPV-58 E6 and E7 in Southwest China
  19. 2759
    “…Phylogenetic trees were constructed on the concatenated amino acid sequences of 13 gene products using MEGA v.6.06. RESULTS: The results showed that the six ovine Babesia apicoplast genomes consisted of circular DNA. …”
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    Comparative analysis of apicoplast genomes of Babesia infective to small ruminants in China
  20. 2760
    “…Homological and phylogenetic analyses of the partial NS5B gene were performed using BLAST and MEGA version 6. RESULTS: Based on the phylogenetic tree analysis using 360 nucleotides as the partial NS5B gene, Indonesian BVDV-1 isolates from Central and East Java were subdivided to BVDV-1a (n=9), BVDV-1b (n=1), and BVDV-1c (n=5). …”
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    Genetic analysis of NS5B gene from bovine viral diarrhea virus-infected cattle in Central and East Java, Indonesia
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