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  1. 1581
    “…Drastic speed increase, through complete re-design of its working mechanism, makes VarioWatch capable of annotating millions of human genomic variants generated from next generation sequencing in minutes, if not seconds. While using MegaQuery of VarioWatch to quickly annotate variants, users can apply various filters to retrieve a subgroup of variants according to the risk levels, interested regions, etc. that satisfy users’ requirements. …”
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    VarioWatch: providing large-scale and comprehensive annotations on human genomic variants in the next generation sequencing era
  2. 1582
    “…Conclusion The lessons identified from this mega-disaster highlighted the specific health needs of the vulnerable populations, particularly the elderly and those with non-communicable diseases. …”
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    The Great East Japan Earthquake Disaster: a Compilation of Published Literature on Health Needs and Relief Activities, March 2011-September 2012
  3. 1583
    “…Subtypes were assigned using REGA HIV-1 subtyping tool and phylogenetic trees constructed using MEGA version 5. RESULTS: Although mutations associated with resistance to all three drug were detected in these surveys, transmitted resistance was analyzed and classified as <5% in Maputo in both surveys for all three drug classes. …”
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    Evolution of Primary HIV Drug Resistance in a Subtype C Dominated Epidemic in Mozambique
  4. 1584
    “…After alignment, the significance of amino acid variation was determined by using the χ(2) test and a phylogenetic tree was constructed with MEGA software (version 5.1). RESULTS: We showed no significant difference in neurovirulence between genotype B and C. …”
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    The variations of VP1 protein might be associated with nervous system symptoms caused by enterovirus 71 infection
  5. 1585
    “…METHODS: The rectum was manually outlined on the kilo-voltage planning scan and 37 daily mega-voltage image guidance scans for 10 participants recruited to the VoxTox study. …”
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    Random variation in rectal position during radiotherapy for prostate cancer is two to three times greater than that predicted from prostate motion
  6. 1586
    “…CONCLUSION: Oesophagectomy should be considered for patients with end-stage achalasia and mega-oesophagus causing respiratory compromise to avoid potential fatal complications such as tracheal compression and subsequent respiratory arrest.…”
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    Recurrent respiratory distress and cardiopulmonary arrest caused by megaoesophagus secondary to achalasia()
  7. 1587
    “…This review also describes the different aspects of virology, pathogenesis, epidemiology, and clinical symptoms on the basis of the published article surveillance data and phylogenetic analyses of viral sequences of large, medium, and small segments retrieved from database using mega 5.05, simplot 3.5.1, network 4.611, and epi information system 3.5.3 software. …”
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    Systematic review of severe fever with thrombocytopenia syndrome:virology, epidemiology, and clinical characteristics
  8. 1588
    “…The aligned sequences, 658 base pairs in length, were analyzed for divergence using the Kimura-2-parameter (K2P) distance model with MEGA5.0. RESULTS: The mean intraspecific K2P distance was 0.0103 and the average interspecific genetic distance was 0.2178 in B. multicinctus, far greater than the minimal interspecific genetic distance of 0.027 recommended for species identification. …”
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    Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication
  9. 1589
    “…The progressive clustering method is expected to be effective for the reorganization of any mega-sized DNA molecules cloned in yeast, facilitating the construction of designer genomes in microbes as well as genomic fragments for genetic engineering of higher eukaryotes.…”
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    Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling
  10. 1590
    “…The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. …”
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    Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China
  11. 1591
    “…MATERIALS AND METHODS: This study was conducted as part of a mega project on tuberculosis by the Infectious and Tropical Diseases Research Centre affiliated to Ahvaz Jundishapur University of Medical Sciences. …”
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    Clinical and Radiographic Manifestations and Treatment Outcome of Pulmonary Tuberculosis in the Elderly in Khuzestan, Southwest Iran
  12. 1592
    “…Data demonstrate that in fingermarks, further improvements to the existing method can be achieved using MEGA-8 as surfactant in higher percentages as well as combinations of different detergents. …”
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    Alternative Surfactants for Improved Efficiency of In Situ Tryptic Proteolysis of Fingermarks
  13. 1593
    “…Communities in Sub-Saharan Africa may not conform to these models, however; physical accessibility, availability of transport, and cost of travel between locations may be variable and severely constrained compared to high-income settings, informal labor movements rather than regular commuting patterns are often the norm, and the rise of mega-cities across the continent has important implications for travel between rural and urban areas. …”
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    Evaluating Spatial Interaction Models for Regional Mobility in Sub-Saharan Africa
  14. 1594
    “…BACKGROUND: SUN(^_^)D, the Strategic Use of New generation antidepressants for Depression, is an assessor-blinded, parallel-group, multicenter pragmatic mega-trial to examine the optimum treatment strategy for the first- and second-line treatments for unipolar major depressive episodes. …”
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    Strategic use of new generation antidepressants for depression: SUN(^_^) D protocol update and statistical analysis plan
  15. 1595
    “…In combination with sequences from GenBank, the sequences were aligned using Clustal W and genetic distances were computed using MEGA V5.1. The success rate of PCR amplification and sequencing was 100%. …”
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    Molecular Identification of Dendrobium Species (Orchidaceae) Based on the DNA Barcode ITS2 Region and Its Application for Phylogenetic Study
  16. 1596
    “…The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. …”
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    Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
  17. 1597
    “…Butterflies and moths are found in all terrestrial environments and require efforts for a better understanding of its mega-diversity. These taxa have been the subject of several studies involving phylogeny, ecology and environmental impacts. …”
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    Butterflies of the Bodoquena Plateau in Brazil (Lepidoptera, Papilionoidea)
  18. 1598
    “…Collectively, these data identify domains of the U6 snRNP that are critical for one of the first steps in assembly of the megaDalton U4/U6.U5 tri-snRNP complex, and lead to a dynamic model for U4/U6 pairing that involves a striking degree of evolved cooperativity between protein and RNA.…”
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    Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly
  19. 1599
    “…However, the rs2232710 SNV showed no association with DVT in two Dutch replication cohorts the LETS study (454 patients and 451 controls) and the MEGA study (3799 patients and 4399 controls), indicating that the rs2232710 variant is not a risk factor for DVT.…”
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    Single Nucleotide Variant rs2232710 in the Protein Z-Dependent Protease Inhibitor (ZPI, SERPINA10) Gene Is Not Associated with Deep Vein Thrombosis
  20. 1600
    “…MATERIALS AND METHODS: One hundred five greater taper Ni-Ti instruments were used including Protaper universal hand (Dentsply Maillefer, Ballaigues, Switzerland), Protaper universal rotary (Dentsply Maillefer, Ballaigues, Switzerland), and Revo-S rotary (MicroMega, Besançon, France) files. Files were used on lower anterior teeth. …”
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    Stereomicroscopic evaluation of defects caused by torsional fatigue in used hand and rotary nickel-titanium instruments
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