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201por Kim, Hyaekang, Chung, Sung Hee, Kim, Hyun Soo, Kim, Han-Sung, Song, Wonkeun, Hong, Ki Ho, Kim, Jae-Seok“…METHODS: We performed whole-genome sequencing using Oxford Nanopore MinION sequencing on 58 SARS-CoV-2 isolates collected during the ongoing coronavirus disease 2019 pandemic at a tertiary hospital in South Korea and tracked the emergence of mutations responsible for massive spikes in South Korea. …”
Publicado 2022
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202por Bizzarri, Melissa, Cassanelli, Stefano, Pryszcz, Leszek P., Gawor, Jan, Gromadka, Robert, Solieri, Lisa“…Illumina and Oxford Nanopore MinION sequencing revealed genome sizes of 20.9 and 24.7 Mb, respectively. …”
Publicado 2018
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203“…Serratia liquefaciens strain S1 was isolated from mixed greens and sequenced with short-read Illumina MiSeq technology and long-read MinION technology. Hybrid sequence assembly generated a complete single circular chromosome and two extrachromosomal contigs, which together encoded 5,098 proteins, 85 tRNAs, and 22 rRNAs.…”
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204por Ishino, Masato, Omi, Masatoshi, Araki-Sasaki, Kaoru, Oba, Shimpei, Yamada, Haruhiko, Matsuo, Yoshiyuki, Hirota, Kiichi, Takahashi, Kanji“…PURPOSE: To evaluate the efficacy of identifying the bacteria by aqueous sampling and vitreous sampling in postoperative infectious endophthalmitis using 16S ribosomal RNA (rRNA) gene analysis with a nanopore sequencer (MinION™). OBSERVATION: A 55-year-old woman who underwent cataract surgery at an ophthalmology clinic 18 days ago was referred to our hospital for suspected endophthalmitis. …”
Publicado 2022
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205por Fuselli, S., Baptista, R. P., Panziera, A., Magi, A., Guglielmi, S., Tonin, R., Benazzo, A., Bauzer, L. G., Mazzoni, C. J., Bertorelle, G.“…One of these amplicons was sequenced with the MinION device, which produced long reads covering the entire amplified fragment. …”
Publicado 2018
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206por Bainomugisa, Arnold, Duarte, Tania, Lavu, Evelyn, Pandey, Sushil, Coulter, Chris, Marais, Ben J., Coin, Lachlan M.“…Here, we report the use of the MinION nanopore sequencer (Oxford Nanopore Technologies) to sequence and assemble an extensively drug-resistant (XDR) isolate, which is part of a modern Beijing sub-lineage strain, prevalent in Western Province, Papua New Guinea. …”
Publicado 2018
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207“…In the present study, Oxford Nanopore MinION long-read sequencing was employed to generate full-length SSU rRNA sequences for seven new Blastocystis subtypes for which no full-length references currently exist: ST21, ST23, ST24, ST25, ST26, ST27, and ST28. …”
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208por Brinkmann, Annika, Ulm, Sophie-Luisa, Uddin, Steven, Förster, Sophie, Seifert, Dominique, Oehme, Rainer, Corty, Merle, Schaade, Lars, Michel, Janine, Nitsche, Andreas“…It can be used on the miniature-sized and field-deployable sequencing device Oxford Nanopore MinION, with sequencing library preparation time of 10 min. …”
Publicado 2021
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209por Pechtl, Alexander, Rückert, Christian, Maus, Irena, Koeck, Daniela E., Trushina, Nataliya, Kornberger, Petra, Schwarz, Wolfgang H., Schlüter, Andreas, Liebl, Wolfgang, Zverlov, Vladimir V.Enlace del recurso
Publicado 2018
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210por Brinkmann, Annika, Uddin, Steven, Ulm, Sophie-Luisa, Pape, Katharina, Förster, Sophie, Enan, Khalid, Nourlil, Jalal, Krause, Eva, Schaade, Lars, Michel, Janine, Nitsche, Andreas“…Here we present a rapid multiplex approach (RespiCoV), combining highly multiplexed PCRs and MinION sequencing suitable for the simultaneous screening for 41 viral and five bacterial agents related to respiratory tract infections, including the human coronaviruses NL63, HKU1, OC43, 229E, Middle East respiratory syndrome coronavirus, SARS-CoV, and SARS-CoV-2. …”
Publicado 2022
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211por Natali, Susan. M., Watts, Jennifer D., Rogers, Brendan M., Potter, Stefano, Ludwig, Sarah M., Selbmann, Anne-Katrin, Sullivan, Patrick F., Abbott, Benjamin W., Arndt, Kyle A., Birch, Leah, Björkman, Mats P., Bloom, A. Anthony, Celis, Gerardo, Christensen, Torben R., Christiansen, Casper T., Commane, Roisin, Cooper, Elisabeth J., Crill, Patrick, Czimczik, Claudia, Davydov, Sergey, Du, Jinyang, Egan, Jocelyn E., Elberling, Bo, Euskirchen, Eugenie S., Friborg, Thomas, Genet, Hélène, Göckede, Mathias, Goodrich, Jordan P., Grogan, Paul, Helbig, Manuel, Jafarov, Elchin E., Jastrow, Julie D., Kalhori, Aram A. M., Kim, Yongwon, Kimball, John, Kutzbach, Lars, Lara, Mark J., Larsen, Klaus S., Lee, Bang-Yong, Liu, Zhihua, Loranty, Michael M., Lund, Magnus, Lupascu, Massimo, Madani, Nima, Malhotra, Avni, Matamala, Roser, McFarland, Jack, McGuire, A. David, Michelsen, Anders, Minions, Christina, Oechel, Walter C., Olefeldt, David, Parmentier, Frans-Jan W., Pirk, Norbert, Poulter, Ben, Quinton, William, Rezanezhad, Fereidoun, Risk, David, Sachs, Torsten, Schaefer, Kevin, Schmidt, Niels M., Schuur, Edward A.G., Semenchuk, Philipp R., Shaver, Gaius, Sonnentag, Oliver, Starr, Gregory, Treat, Claire C., Waldrop, Mark P., Wang, Yihui, Welker, Jeffrey, Wille, Christian, Xu, Xiaofeng, Zhang, Zhen, Zhuang, Qianlai, Zona, DonatellaEnlace del recurso
Publicado 2019
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212“…Here, we validate the MinION’s application to heterozygous crops by creating a de novo genome assembly of a key parent in breeding programs, the clone Pound 7. …”
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213por Latorre-Pérez, Adriel, Villalba-Bermell, Pascual, Pascual, Javier, Vilanova, Cristina“…Overall, nanopore metagenomic sequencing data-adapted to MinION’s current output-proved sufficient for assembling and characterizing low-complexity microbial communities.…”
Publicado 2020
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214por Cabibbe, Andrea M., Spitaleri, Andrea, Battaglia, Simone, Colman, Rebecca E., Suresh, Anita, Uplekar, Swapna, Rodwell, Timothy C., Cirillo, Daniela M.“…In conclusion, we demonstrated that the workflow and sequencing data obtained from Deeplex on MinION are comparable to those for the MiniSeq, despite the higher raw error rates on MinION, with the added advantage of MinION’s portability, versatility, and low capital costs. …”
Publicado 2020
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215por Kang, Albert S. W., Bernasconi, Janette G., Jack, William, Kanavarioti, Anastassia“…Intact oligos traverse the MinION’s nanopore relatively quickly compared to the device’s acquisition rate, and exhibit count of events comparable to the baseline. …”
Publicado 2020
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216por Tyler, Andrea D., McAllister, Jane, Stapleton, Helen, Gauci, Penny, Antonation, Kym, Thirkettle-Watts, David, Corbett, Cindi R.“…From pathogen detection to genome or plasmid closure, the utility of the Oxford Nanopore Technologies (ONT) MinION for microbiological analysis has been well documented. …”
Publicado 2023
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217por Runtuwene, Lucky R., Sathirapongsasuti, Nuankanya, Srisawat, Raweewan, Komalamisra, Narumon, Tuda, Josef S. B., Mongan, Arthur E., Aboge, Gabriel O., Shabardina, Victoria, Makalowski, Wojciech, Nesti, Dela Ria, Artama, Wayan T., Nguyen-Thi, Lan Anh, Wan, Kiew-Lian, Na, Byoung-Kuk, Hall, William, Pain, Arnab, Eshita, Yuki, Maeda, Ryuichiro, Yamagishi, Junya, Suzuki, Yutaka“…OBJECTIVE: To disseminate the portable sequencer MinION in developing countries for the main purpose of battling infectious diseases, we found a consortium called Global Research Alliance in Infectious Diseases (GRAID). …”
Publicado 2022
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218por Voorhuijzen-Harink, Marleen M., Hagelaar, Rico, van Dijk, Jeroen P., Prins, Theo W., Kok, Esther J., Staats, Martijn“…• MinION DNA metabarcoding is a promising tool for species identification in food. • MinION and Illumina MiSeq sequencing platforms perform equally accurate. • Species identification with MinION sequencing requires dedicated bioinformatics.…”
Publicado 2019
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219por Nilgiriwala, Kayzad, Rabodoarivelo, Marie-Sylvianne, Hall, Michael B., Patel, Grishma, Mandal, Ayan, Mishra, Shefali, Andrianomanana, Fanantenana Randria, Dingle, Kate, Rodger, Gillian, George, Sophie, Crook, Derrick W., Hoosdally, Sarah, Mistry, Nerges, Rakotosamimanana, Niaina, Iqbal, Zamin, Grandjean Lapierre, Simon, Walker, Timothy M.“…The fraction of M. tuberculosis reads from MinION sequencing was lower than from Illumina, but monoplexing grade 3+ samples on MinION produced higher read depth than Illumina (P < 0.05) and MinION multiplexing (P < 0.01). …”
Publicado 2023
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220por Filloux, Denis, Fernandez, Emmanuel, Loire, Etienne, Claude, Lisa, Galzi, Serge, Candresse, Thierry, Winter, Stephan, Jeeva, M. L., Makeshkumar, T., Martin, Darren P., Roumagnac, Philippe“…We here assessed the capability of the MinION sequencing approach to detect and characterize viruses infecting a water yam plant. …”
Publicado 2018
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