Mostrando 241 - 260 Resultados de 989 Para Buscar '"Minions"', tiempo de consulta: 0.13s Limitar resultados
  1. 241
    “…In this study, we evaluated the use of the MinION real-time sequencer as well as different polishing tools on MinION-sequenced genome for sequencing the MPXV genome originating from a pustular lesion in the context of an epidemic in a remote area of the Central African Republic. …”
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    Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic
  2. 242
    “…BTx623 while using the MinION platform and obtained 895,678 reads and 17.9 gigabytes (Gb) (ca. 25× coverage of reference) from long-read sequence data. …”
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    Constructing a Reference Genome in a Single Lab: The Possibility to Use Oxford Nanopore Technology
  3. 243
    “…Here, we describe a rapid, inexpensive, and portable strategy to robustly re-identify human DNA called 'MinION sketching'. MinION sketching requires as few as 3 min of sequencing and 60-300 random SNPs to re-identify a sample enabling near real-time applications of DNA re-identification. …”
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    Rapid re-identification of human samples using portable DNA sequencing
  4. 244
    “…The average accuracy of NaS reads reached 99.99% without losing the initial size of the input MinION® reads. CONCLUSIONS: We described NaS tool, a hybrid approach allowing the sequencing of microbial genomes using the MinION® device. …”
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    Genome assembly using Nanopore-guided long and error-free DNA reads
  5. 245
    “…Here we demonstrate rapid and accurate sequencing of two disease-causing viruses affecting global salmonid aquaculture, salmonid alphavirus (SAV) and infectious salmon anaemia virus (ISAV), using third-generation nanopore sequencing on the MinION platform (Oxford Nanopore Technologies). Our approach complements PCR from infected material with MinION sequencing to recover genomic information that matches near perfectly to Sanger-verified references. …”
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    Nanopore sequencing for rapid diagnostics of salmonid RNA viruses
  6. 246
    “…However, the accuracy of MinION, per base, is much lower than MiSeq (95% versus 99.9%). …”
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    Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil
  7. 247
    “…In field-collected clinical swab samples, NDV (Ct range 12–37) was detected in all eleven samples within 1 h of MinION sequencing, with 10 of 11 samples accurately genotyped within 1 h. …”
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    Comparable outcomes from long and short read random sequencing of total RNA for detection of pathogens in chicken respiratory samples
  8. 248
    “…We developed a protocol using ONT’ MinION sequencer to accurately detect and classify a wide spectrum of VBB from canine blood at a sensitivity comparable to that of regularly used diagnostics, such as qPCR. …”
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    Nanopore Sequencing Using the Full-Length 16S rRNA Gene for Detection of Blood-Borne Bacteria in Dogs Reveals a Novel Species of Hemotropic Mycoplasma
  9. 249
    “…Using low coverage ONT minION sequencing for genotyping of pre-validated SNP loci was examined in 9 canola doubled haploids. …”
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    Assessment of low-coverage nanopore long read sequencing for SNP genotyping in doubled haploid canola (Brassica napus L.)
  10. 250
    “…Moreover, we adjusted TSC for the Oxford Nanopore MinION and compared the performance to an SMg approach. …”
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    Assessment of Viral Targeted Sequence Capture Using Nanopore Sequencing Directly from Clinical Samples
  11. 251
    “…Due to its low equipment cost, short turn-around-time, and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.…”
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    Rapid diagnosis of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis by next-generation sequencing: a case report
  12. 252
    “…It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to be isolated from the tissue biopsy. …”
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    Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
  13. 253
    “…In this study, Oxford nanopore MinION sequencing was carried out to generate complete genomes of PPRV from archived PPRV-positive samples collected from PPR outbreaks in goats in Ngorongoro and Momba districts in Tanzania during 2016 and 2018, respectively. …”
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    Complete Genome Sequencing of Field Isolates of Peste des Petits Ruminants Virus from Tanzania Revealed a High Nucleotide Identity with Lineage III PPR Viruses
  14. 254
    “…Nanopore sequencing was recently made available to users in the form of the Oxford Nanopore MinION. Released to users through an early access programme, the MinION is made unique by its tiny form factor and ability to generate very long sequences from single DNA molecules. …”
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    A disruptive sequencer meets disruptive publishing
  15. 255
    “…In this study, MinION reads were generated from the multi-chromosome genome of Agrobacterium tumefaciens strain LBA4404. …”
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    Characterization, correction and de novo assembly of an Oxford Nanopore genomic dataset from Agrobacterium tumefaciens
  16. 256
    “…The performance of the MinION sequencer and the portable laboratory was tested for DNA barcoding in a mimicking tropical environment, as well as in a remote rainforest of Tanzania lacking electricity. …”
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    On site DNA barcoding by nanopore sequencing
  17. 257
    “…Different 16S gene regions were amplified, bar-coded, and sequenced using the Illumina MiSeq and ONT MinION sequencers and corresponding kits. Mapping of the sequenced amplicon using MinION to the entire 16S rRNA gene was analyzed with the cloud-based EPI2ME algorithm. …”
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    Characterization of Fecal Microbiota with Clinical Specimen Using Long-Read and Short-Read Sequencing Platform
  18. 258
    “…To ease future molecular studies, this study compares first- (partial sequencing via Sanger), second- (Illumina), and third-generation (MinION Nanopore) sequencing platforms for USUV. With emphasis on MinION Nanopore sequencing, cDNA-direct and target-enrichment (amplicon-based) sequencing approaches were validated in parallel. …”
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    Expedited retrieval of high-quality Usutu virus genomes via Nanopore sequencing with and without target enrichment
  19. 259
    “…The MinION™ is a USB-connected, portable nanopore sequencer that permits real-time analysis of streaming event data. …”
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    Poretools: a toolkit for analyzing nanopore sequence data
  20. 260
    “…The combination of Illumina RNASeq and MinION nanopore technologies enabled the detection of at least one transcript for each protein-coding gene encoded by Chr 18. …”
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    Genome of the Single Human Chromosome 18 as a “Gold Standard” for Its Transcriptome
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