-
361por Rand, Arthur C., Jain, Miten, Eizenga, Jordan M., Musselman-Brown, Audrey, Olsen, Hugh E., Akeson, Mark, Paten, Benedict“…We present a framework for mapping methylation to cytosine and adenosine with the Oxford Nanopore Technologies MinION using its ionic current signal. We map three cytosine variants and two adenine variants. …”
Publicado 2017
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
362por Pachi Pulusu, Chanakya, Khamari, Balaram, Lama, Manmath, Peketi, Arun Sai Kumar, Kumar, Prakash, Nagaraja, Valakunja, Bulagonda, Eswarappa Pradeep“…The genome assembly was generated by combining short-read Illumina HiSeq-X Ten and long-read Oxford Nanopore Technologies MinION sequence data using the Unicycler assembler.…”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
363por Mannan, Adnan, Mehedi, H. M. Hamidullah, Rob, M. Abdur, Biswas, Sanjoy Kanti, Sultana, Nahid, Biswas, Rajdeep, Hoque, M. Minhazul, Hossain, Mohammed Akram, Das, Ajoy, Chakma, Kallyan, Salauddin, Asma, Raza, M. Thosif, Reza, Fahim Hasan, Mahtab, Azmain, Miah, Mojnu, Hasan, Rashedul, Rahman, Mustafizur, Rahman, Mohammed Ziaur, Hossain, Mohammad Enayet“…We announce the complete genome sequences of 12 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sublineage B.1.617.2 strains (Delta variant) obtained from nasopharyngeal and oropharyngeal swab samples from 12 pediatric patients in Chittagong, Bangladesh, displaying COVID-19 symptoms. Oxford Nanopore MinION sequencing technology was used to generate the genomic sequences.…”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
364por Ravi, Keerthikka, Rauch, Marcus, Lynch, Susan V., Lukacs, Nicholas W., Huffnagle, Gary B.“…Long-read sequencing of this isolate, which was performed using a MinION sequencer, yielded a single, closed genome of 1,953,837 bp, with a GC content of 34.67%.…”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
365por Naômé, Aymeric, Maciejewska, Marta, Calusinska, Magdalena, Martinet, Loïc, Anderssen, Sinaeda, Adam, Delphine, Tenconi, Elodie, Deflandre, Benoit, Coppieters, Wouter, Karim, Latifa, Hanikenne, Marc, Baurain, Denis, Delfosse, Philippe, van Wezel, Gilles P., Rigali, Sébastien“…The complete genome sequence of MM109(T) was obtained by combining Oxford Nanopore MinION and Illumina HiSeq and MiSeq technologies, revealing an 8.4-Mb linear chromosome and two plasmids, pSLUN1 (127,264 bp, linear) and pSLUN2 (46,827 bp, circular).…”
Publicado 2018
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
366por D’Souza, Roshan, Filippov, Andrey A., Sergueev, Kirill V., He, Yunxiu, Ward, Amanda M., Goglin, Karrie, Vashee, Sanjay, Nikolich, Mikeljon P., Fouts, Derrick E.“…We report the complete genome sequence of ESa1, assembled using both MinION and Illumina MiSeq reads, consisting of 153,106 bp, with 30.3% GC content, 253 protein coding sequences, 4 tRNAs, and 10,437-bp direct terminal repeats.…”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
367“…MOTIVATION: The introduction of portable DNA sequencers such as the Oxford Nanopore Technologies MinION has enabled real-time and in the field DNA sequencing. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
368
-
369“…Our study indicates that direct mtDNA sequencing from native DNA on the MinION™ device provides comparable results to those obtained from common mtDNA sequencing methods and is a reliable alternative to approaches using PCR‐enriched libraries.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
370“…In this study, a total of nine Escherichia coli and Klebsiella pneumoniae isolates from Norwegian clinical samples were sequenced using both MinION and Illumina platforms. Three out of nine samples were sequenced directly from blood culture, and one sample was sequenced from a mixed-blood culture. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
371por Phelan, Jody E., O’Sullivan, Denise M., Machado, Diana, Ramos, Jorge, Oppong, Yaa E. A., Campino, Susana, O’Grady, Justin, McNerney, Ruth, Hibberd, Martin L., Viveiros, Miguel, Huggett, Jim F., Clark, Taane G.“…The predictive performance of the mutation library has been assessed using > 17,000 clinical isolates with WGS and laboratory-based drug susceptibility testing (DST) data. An integrated MinION analysis pipeline was assessed by performing WGS on 34 replicates across 3 multi-drug resistant isolates with known resistance mutations. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
372por Irwin, Adam D., Coin, Lachlan J. M., Harris, Patrick N. A., Cotta, Menino Osbert, Bauer, Michelle J., Buckley, Cameron, Balch, Ross, Kruger, Peter, Meyer, Jason, Shekar, Kiran, Brady, Kara, Fourie, Cheryl, Sharp, Natalie, Vlad, Luminita, Whiley, David, Beatson, Scott A., Forde, Brian M., Paterson, David, Clark, Julia, Hajkowicz, Krispin, Raman, Sainath, Bialasiewicz, Seweryn, Lipman, Jeffrey, Schlapbach, Luregn J., Roberts, Jason A.“…In Phase 1, we will evaluate Oxford Nanopore Technologies MinION sequencing direct from blood in 50 blood culture-proven sepsis patients recruited from consecutive patients with suspected sepsis. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
373por D’Souza, Roshan, White, Richard C., Buzzeo, Rachel, Goglin, Karrie, Vashee, Sanjay, Lee, Yoona, Son, Bokyung, Ryu, Sangryeol, Fouts, Derrick E.“…We used a hybrid approach combining MinION and Illumina MiSeq sequencing, which yielded a 43,134-bp genome and 65 open reading frames.…”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
374por Chanda, Bidisha, Rivera, Yazmín, Nunziata, Schyler O., Galvez, Marco E., Gilliard, Andrea, Ling, Kai-Shu“…The complete genome sequence of a U.S. isolate of a Tomato brown rugose fruit virus (ToBRFV) (CA18-01) was obtained through Illumina and MinION sequencing. The U.S. ToBRFV isolate shared a high nucleic acid sequence identity (>99%) with known ToBRFV isolates. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
375por Ravi, Keerthikka, Erb-Downward, John R., Gammon, Natalie K., Falkowski, Nicole R., Huffnagle, Gary B.“…Long-read sequencing was performed using a MinION sequencer, and reads were assembled into circular chromosomes of 4,842,187 bp and 4,814,486 bp for M101 and M105, respectively. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
376por Zainulabid, Ummu Afeera, Mohamad Zain, Norirwanisyam, Arumugam, Janaki, Kamarudin, Norhidayah, Zainal Abidin, Muhammad ‘Adil, Abd Mokti, Abdullah Shamshir, Nordin, Noorlina, Faizal Rakawi, Ahmad, Abdul Majid, Aman Shah, Ashok, Gnanasekaran, Francis, Alex Lourdes, Tay, Darren Dean, Vijayalakshami, Natesan, Hin, How Soon, Ahmad, Hajar Fauzan“…Here, we present two 7.1- and 7.3-bp near-complete genome sequences of Burkholderia pseudomallei strains of HTAA077 and HRPB058, isolated from a pus culture from a confirmed melioidosis patient at Hospital Tengku Ampuan Afzan, Kuantan, Malaysia, and from blood culture from a patient at Hospital Raja Permaisuri Bainun, Ipoh, Malaysia, using a Nanopore MinION instrument.…”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
377por Su, Junhao, Lui, Wui Wang, Lee, YanLam, Zheng, Zhenxian, Siu, Gilman Kit-Hang, Ng, Timothy Ting-Leung, Zhang, Tong, Lam, Tommy Tsan-Yuk, Lao, Hiu-Yin, Yam, Wing-Cheong, Tam, Kingsley King-Gee, Leung, Kenneth Siu-Sing, Lam, Tak-Wah, Leung, Amy Wing-Sze, Luo, Ruibang“…Oxford nanopore technologies (ONT) MinION sequencing allows rapid and simple sample preparation for sequencing. …”
Publicado 2023
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
378por Castro-Wallace, Sarah L., Chiu, Charles Y., John, Kristen K., Stahl, Sarah E., Rubins, Kathleen H., McIntyre, Alexa B. R., Dworkin, Jason P., Lupisella, Mark L., Smith, David J., Botkin, Douglas J., Stephenson, Timothy A., Juul, Sissel, Turner, Daniel J., Izquierdo, Fernando, Federman, Scot, Stryke, Doug, Somasekar, Sneha, Alexander, Noah, Yu, Guixia, Mason, Christopher E., Burton, Aaron S.“…We evaluated the performance of the MinION DNA sequencer in-flight on the International Space Station (ISS), and benchmarked its performance off-Earth against the MinION, Illumina MiSeq, and PacBio RS II sequencing platforms in terrestrial laboratories. …”
Publicado 2017
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
379por Theuns, Sebastiaan, Vanmechelen, Bert, Bernaert, Quinten, Deboutte, Ward, Vandenhole, Marilou, Beller, Leen, Matthijnssens, Jelle, Maes, Piet, Nauwynck, Hans J.“…First, cell culture-grown porcine epidemic diarrhea virus and rotavirus A were pooled and sequenced on a MinION. Reads were already detected at 7 seconds after start of sequencing, resulting in high sequencing depths (19.2 to 103.5X) after 3 h. …”
Publicado 2018
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
380por Briggs, Benjamin, Federman, Scot, Deng, Xian Ding, Gu, Wei, Yu, Guixia, Martin, Claudia Sanchez San, Miller, Steve, Chiu, Charles Y“…RESULTS: With the use of molecular concatemerization library approach, we show that metagenomic and transcriptomic data generated on the MinION are comparable to those on the Illumina platform, yet can be collected and analyzed in significantly less time (6 hours vs. 2–3 days). …”
Publicado 2018
Enlace del recurso
Enlace del recurso
Online Artículo Texto