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381por Kafetzopoulou, Liana E., Efthymiadis, Kyriakos, Lewandowski, Kuiama, Crook, Ant, Carter, Dan, Osborne, Jane, Aarons, Emma, Hewson, Roger, Hiscox, Julian A., Carroll, Miles W., Vipond, Richard, Pullan, Steven T.“…METHODS: We performed metagenomic sequencing using both the Illumina MiSeq and the portable Oxford Nanopore MinION on clinical samples which were real-time reverse transcription-PCR (qRT-PCR) positive for chikungunya (CHIKV) or dengue virus (DENV), two of the most important arboviruses. …”
Publicado 2018
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382“…Due its notable characteristics, researchers have recently started performing assessments regarding the suitability MinION on 16S rRNA sequencing studies, and have obtained remarkable results. …”
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383por Leidenfrost, Robert M., Bänsch, Svenja, Prudnikow, Lisa, Brenig, Bertram, Westphal, Catrin, Wünschiers, Röbbe“…We compared next-generation (Illumina), i.e., short-read, and third-generation (Nanopore), i.e., MinION, sequencing techniques. MinION data were preprocessed using traditional and Nanopore specific tools for comparative analysis and were evaluated in comparison to short-read sequence data with conventional processing. …”
Publicado 2020
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384por De Vivo, Mattia, Lee, Hsin-Han, Huang, Yu-Sin, Dreyer, Niklas, Fong, Chia-Ling, de Mattos, Felipe Monteiro Gomes, Jain, Dharmesh, Wen, Yung-Hui Victoria, Mwihaki, John Karichu, Wang, Tzi-Yuan, Machida, Ryuji J., Wang, John, Chan, Benny K. K., Tsai, Isheng Jason“…Approximately 4 Gb of sequence was produced from 16 Flongle and one MinION flow cells, averaging 235 Mb and N50 = 4.4 kb per flow cell. …”
Publicado 2022
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385“…The genome was assembled using nanopore MinION readings, yielding an assembly of 8,857,650 base pairs made up of 5(five) contigs. …”
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386por Boukerb, Amine M., Schaeffer, Julien, Serghine, Joëlle, Carrier, Gregory, Le Guyader, Françoise S., Gourmelon, Michèle“…As determined by a hybrid approach combining Oxford Nanopore MinION and Illumina MiniSeq sequence data, Campylobacter armoricus strain CA639 harbored a circular chromosome of 1,688,169 bp with a G+C content of 28.47% and two plasmids named pCA639-1 and pCA639-2, with lengths of 51,123 and 28,139 bp, and G+C contents of 26.5% and 28.45%, respectively.…”
Publicado 2020
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387por Nita, I. B., Ilie, O. D., Ciobica, A. S., Hritcu, L. D., Popescu, R., Dobrin, R. P.“…For “schizophrenia” + “MinION, there was a cumulative number of n = 5, from which we had n = 0 in PubMed/Medline, n = 0 in ISI Web of Knowledge, n = 0 in Scopus, and n = 5 in ScienceDirect. …”
Publicado 2023
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388por Runtuwene, Lucky R., Tuda, Josef S. B., Mongan, Arthur E., Makalowski, Wojciech, Frith, Martin C., Imwong, Mallika, Srisutham, Suttipat, Nguyen Thi, Lan Anh, Tuan, Nghia Nguyen, Eshita, Yuki, Maeda, Ryuichiro, Yamagishi, Junya, Suzuki, Yutaka“…Here, we report the application of a portable sequencer, MinION, for genotyping the malaria parasite Plasmodium falciparum. …”
Publicado 2018
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389por Acharya, Kishor, Blackburn, Adrian, Mohammed, Jemila, Haile, Alemseged Tamiru, Hiruy, Andualem Mekonnen, Werner, David“…We describe the technical feasibility of metagenomic water quality analysis using only portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Technologies, for the library preparation and sequencing of 16S rRNA gene amplicons. …”
Publicado 2020
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390“…We obtained 922,210 reads using MinION with genomic DNA from a transgenic mouse strain (4C30, Proc. …”
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391por Avershina, Ekaterina, Sharma, Priyanka, Taxt, Arne M., Singh, Harpreet, Frye, Stephan A., Paul, Kolin, Kapil, Arti, Naseer, Umaer, Kaur, Punit, Ahmad, Rafi“…Moreover, they can be used both on Illumina and MinION generated data and do not require high genome coverage for phenotype prediction. …”
Publicado 2021
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392por Deeg, Christoph M., Sutherland, Ben J. G., Ming, Tobi J., Wallace, Colin, Jonsen, Kim, Flynn, Kelsey L., Rondeau, Eric B., Beacham, Terry D., Miller, Kristina M.“…However, recent advances in third‐generation single‐molecule sequencing platforms, such as the Oxford Nanopore minION, provide base calling on portable, pocket‐sized sequencers and promise real‐time, in‐field stock identification of variable batch sizes. …”
Publicado 2022
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393por Gurgul, Artur, Jasielczuk, Igor, Szmatoła, Tomasz, Sawicki, Sebastian, Semik-Gurgul, Ewelina, Długosz, Bogusława, Bugno-Poniewierska, Monika“…In this study, we attempt to develop a Nanopore sequencing-based genotyping strategy (with MinION system from Oxford Nanopore) that allows simple and cost-efficient genome-wide analysis in horse species. …”
Publicado 2023
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395“…The Oxford Nanopore MinION sequences DNA by sensing changes in electrical current flow in real-time as molecules traverse nanopores. …”
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396por da Costa, Vanessa Duarte, Santos, Alanna Calheiros, da Silva, Lucas Limas, Wiggers, Wilian Jean, Ivantes, Claudia Alexandra Pontes, Lima, Danielle Malta, Colares, Jeová Keny Baima, Dallacqua, Deusilene Souza Vieira, Motta-Castro, Ana Rita Coimbra, de Paula, Vanessa Salete, Dávila, Alberto Martín Rivera, Pollo-Flores, Priscilla, Lewis-Ximenez, Lia Laura, Villar, Livia Melo“…Besides, RT-LAMP was capable of detecting all SARS-CoV-2 lineages genotyped by MinION in both groups.…”
Publicado 2023
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397“…Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 ‘full-length’ isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. …”
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398por Postigo-Hidalgo, Ignacio, Fischer, Carlo, Moreira-Soto, Andres, Tscheak, Patricia, Nagel, Michael, Eickmann, Markus, Drexler, Jan Felix“…We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. …”
Publicado 2020
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399por Pomerantz, Aaron, Peñafiel, Nicolás, Arteaga, Alejandro, Bustamante, Lucas, Pichardo, Frank, Coloma, Luis A, Barrio-Amorós, César L, Salazar-Valenzuela, David, Prost, Stefan“…We used portable equipment, including the MinION nanopore sequencer (Oxford Nanopore Technologies) and the miniPCR (miniPCR), to perform DNA extraction, polymerase chain reaction amplification, and real-time DNA barcoding of reptile specimens in the field. …”
Publicado 2018
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400“…All tested tools were initially developed for short NGS reads, but some have claimed support for long Pacific Biosciences (PacBio) or even Oxford Nanopore Technologies (ONT) MinION reads. RESULTS: The tools were tested on synthetic and real datasets from two technologies (PacBio and ONT MinION). …”
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