-
401“…We will use a third-generation sequencing platform (the MinION sequencer from Oxford Nanopore Technology), comparing data from a sample subset to the Illumina platform as a means of measuring viability of the MinION platform in this specific setting. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
402por Krehenwinkel, Henrik, Pomerantz, Aaron, Henderson, James B, Kennedy, Susan R, Lim, Jun Ying, Swamy, Varun, Shoobridge, Juan Diego, Graham, Natalie, Patel, Nipam H, Gillespie, Rosemary G, Prost, Stefan“…CONCLUSIONS: Sequencing dual indexed, long rDNA amplicons on the MinION platform is a straightforward, cost-effective, portable, and universal approach for eukaryote DNA barcoding. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
403por Pitt, Miranda E, Nguyen, Son H, Duarte, Tânia P S, Teng, Haotian, Blaskovich, Mark A T, Cooper, Matthew A, Coin, Lachlan J M“…CONCLUSION: Overall, MinION sequencing rapidly detected the XDR/PDR K. pneumoniae resistome, and direct RNA sequencing provided accurate estimation of expression levels of these genes.…”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
404por Prost, Stefan, Petersen, Malte, Grethlein, Martin, Hahn, Sarah Joy, Kuschik-Maczollek, Nina, Olesiuk, Martyna Ewa, Reschke, Jan-Olaf, Schmey, Tamara Elke, Zimmer, Caroline, Gupta, Deepak K., Schell, Tilman, Coimbra, Raphael, De Raad, Jordi, Lammers, Fritjof, Winter, Sven, Janke, Axel“…The use of ∼35x nanopore-based long-read data sequenced on a MinION platform (Oxford Nanopore Technologies) allowed us to generate a baseline assembly of only 1,276 contigs with a contig N50 of 2.1 Mbp, and a total length of 441 Mbp. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
405por Krasnov, George S., Pushkova, Elena N., Novakovskiy, Roman O., Kudryavtseva, Ludmila P., Rozhmina, Tatiana A., Dvorianinova, Ekaterina M., Povkhova, Liubov V., Kudryavtseva, Anna V., Dmitriev, Alexey A., Melnikova, Nataliya V.“…Sequencing of DNA extracted using this protocol allowed us to obtain about 85x genome coverage with long (N50 = 29 kb) MinION reads and 30x coverage with 2 × 250 bp HiSeq reads. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
406por Gomes-de-Sá, Sónia, Barradas, Patrícia, Queirós-Reis, Luís, Matas, Isabel M., Amorim, Irina, Cardoso, Luís, Muñoz-Mérida, Antonio, Mesquita, João R.“…We expanded the use of long-read nanopore-based sequencing technology by performing genomic de novo assembly of a D. immitis specimen retrieved from a canine cardiopulmonary dirofilariasis case by using the ONT MinION platform. We also identified loci previously characterized as being associated to macrocyclic lactone resistance selection pressure. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
407por Yu, Ming-Chih, Hung, Ching-Sheng, Huang, Chun-Kai, Wang, Cheng-Hui, Liang, Yu-Chih, Lin, Jung-Chun“…In this study, we propose a whole-genome sequencing (WGS) pipeline via the Nanopore MinION platform to facilitate the efficacy of phenotypic identification of clinical isolates. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
408“…In this study, the complete mitochondrial genome of M. gulio was sequenced using the MinION system (Oxford Nanopore Technologies). The mitochondrial genome is 16,518 bp in length (41.1% [G + C] content) and consists of 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
409por Minervini, Crescenzio Francesco, Cumbo, Cosimo, Orsini, Paola, Anelli, Luisa, Zagaria, Antonella, Specchia, Giorgina, Albano, Francesco“…In 2012 Oxford Nanopore Technologies (ONT) released the MinION, the first long-read nanopore-based sequencer, overcoming the main limits of short-reads sequences generation. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
410“…We present the first mitogenome sequence of the Smoothnose Wedgefish, Rhynchobatus laevis obtained through field sequencing on the MinION handheld sequencer. The mitochondrial genome of R. laevis is 16,560 bp in length and consisted of 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a non-coding control region (D-loop). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
411
-
412por Garcia-Segura, Sergio, del Rey, Javier, Closa, Laia, Garcia-Martínez, Iris, Hobeich, Carlos, Castel, Ana Belén, Vidal, Francisco, Benet, Jordi, Oliver-Bonet, Maria“…In the present work, seminal microbiota of 14 control samples and 42 samples of idiopathic infertile patients were characterized based on full-length sequencing of the 16S rRNA gene using MinION platform from Oxford Nanopore. These same samples had been analyzed previously using Illumina’s MiSeq sequencing platform. …”
Publicado 2023
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
413por Votintseva, Antonina A., Bradley, Phelim, Pankhurst, Louise, del Ojo Elias, Carlos, Loose, Matthew, Nilgiriwala, Kayzad, Chatterjee, Anirvan, Smith, E. Grace, Sanderson, Nicolas, Walker, Timothy M., Morgan, Marcus R., Wyllie, David H., Walker, A. Sarah, Peto, Tim E. A., Crook, Derrick W., Iqbal, Zamin“…A critical advantage of MinION is the ability to continue sequencing until sufficient coverage is obtained, providing a potential solution to the problem of variable amounts of M. tuberculosis DNA in direct samples.…”
Publicado 2017
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
414por Liem, Michael, Jansen, Hans J., Dirks, Ron P., Henkel, Christiaan V., van Heusden, G. Paul H., Lemmers, Richard J.L.F., Omer, Trifa, Shao, Shuai, Punt, Peter J., Spaink, Herman P.“…Background : The introduction of the MinION sequencing device by Oxford Nanopore Technologies may greatly accelerate whole genome sequencing. …”
Publicado 2018
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
415“…We also included two field isolates of serotypes 2 and 3 that were PacBio- and MinION-sequenced, respectively. Genome assemblies were performed following two different strategies, i.e. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
416por Jansen, Hans J., Liem, Michael, Jong-Raadsen, Susanne A., Dufour, Sylvie, Weltzien, Finn-Arne, Swinkels, William, Koelewijn, Alex, Palstra, Arjan P., Pelster, Bernd, Spaink, Herman P., Thillart, Guido E. van den, Dirks, Ron P., Henkel, Christiaan V.“…We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. …”
Publicado 2017
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
417“…Here, we are reviewing the works have been undertaken to utilize a portable sequencer, MinION, in various aspects of infectious disease management.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
418por Christopher, Heavenlight, Burns, Adam, Josephat, Emmanuel, Makani, Julie, Schuh, Anna, Nkya, Siana“…RESULTS: Sequencing results for SCD using the MinION were 100% concordant with those from the Sanger method. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
419por Dunn, Nicholas, Johri, Shaili, Curnick, David, Carbone, Chris, Dinsdale, Elizabeth A., Chapple, Taylor K., Block, Barbara A., Savolainen, Vincent“…Two specimens from the British Indian Ocean Territory were sequenced independently using two different next generation sequencing methods, namely short read sequencing on the Illumina HiSeq and long read sequencing on the Oxford Nanopore Technologies’ MinION sequencer. The two sequences are 99.9% identical and are 16,705 base pairs (bp) and 16,706 bp in length. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
420por Moldován, Norbert, Torma, Gábor, Gulyás, Gábor, Hornyák, Ákos, Zádori, Zoltán, Jefferson, Victoria A., Csabai, Zsolt, Boldogkői, Miklós, Tombácz, Dóra, Meyer, Florencia, Boldogkői, Zsolt“…This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto