Mostrando 421 - 440 Resultados de 989 Para Buscar '"Minions"', tiempo de consulta: 0.15s Limitar resultados
  1. 421
    “…Total RNA was sequenced from the samples on the Illumina NextSeq and on the MinION to identify pathogens present. The MinION long read platform was also used to sequence SARS-CoV-2 with the goal of reducing the complexity of variant calling in mixed populations as occurs with short Illumina reads. …”
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    Analysis of metatranscriptomic methods to enable wastewater-based biosurveillance of all infectious diseases
  2. 422
    “…In this study we used the MinION to sequence long-range PCR amplicons from multiple dinoflagellate species with a focus on the genus Alexandrium. …”
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    The Application of Nanopore Sequencing Technology to the Study of Dinoflagellates: A Proof of Concept Study for Rapid Sequence-Based Discrimination of Potentially Harmful Algae
  3. 423
    “…In recent years, portable and easy-to-use molecular-based tools, such as Oxford Nanopore Technologies’ MinION™ sequencer and miniPCR bio’s miniPCR™ thermal cycler, have been validated onboard the ISS. …”
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    Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing
  4. 424
    “…Twelve classical HLA genes were enriched from cDNA of 50 individuals, barcoded, pooled, and sequenced in 10 MinION R9.4 SpotON flow cell runs producing over 30,000 reads per sample. …”
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    Targeted RNA-Based Oxford Nanopore Sequencing for Typing 12 Classical HLA Genes
  5. 425
    “…However, the analysis of genomic sequences obtained from natural populations is challenging and the biological interpretation of sequencing data remains the main issue. The MinION device developed by Oxford Nanopore Technologies (ONT) is able to generate long reads with minimal costs and time requirements. …”
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    ONT-Based Alternative Assemblies Impact on the Annotations of Unique versus Repetitive Features in the Genome of a Romanian Strain of Drosophila melanogaster
  6. 426
    “…In contrast, Oxford Nanopore MinION was a better choice for microbe classification at the genus and species levels as indicated by samples sequenced for full length 16S rRNA. …”
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    Exploring taxonomic and functional microbiome of Hawaiian stream and spring irrigation water systems using Illumina and Oxford Nanopore sequencing platforms
  7. 427
    “…Sequence was performed with MinION equipment and local data analysis methods were used for sequencing and data analysis. …”
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    Rapid identification of pathogens associated with ventilator-associated pneumonia by Nanopore sequencing
  8. 428
    “…We also demonstrate the efficacy of the MinION nanopore sequencer in rapidly characterizing bacterial community structure and identifying the dominant species. …”
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    Bacterial bioburden and community structure of potable water used in the International Space Station
  9. 429
    “…In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. …”
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    Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38
  10. 430
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    Thoracoabdominal aortic aneurysm repair: current endovascular perspectives
  11. 431
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    Correction: Survival of viral pathogens in animal feed ingredients under transboundary shipping models
  12. 432
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    Correction: Survival of viral pathogens in animal feed ingredients under transboundary shipping models
  13. 433
    “…METHODS: We therefore established a MinION-based mNGS pathogens diagnostic platform and evaluated its potential for clinical implementation in UTIs with clinical samples. 213 urine samples from patients with suspected UTIs were included and subjected to mNGS testing using the MinION platform. mNGS results were compared to the gold standard of clinical culture and composite standard of combining clinical testing, confirmatory qPCR testing, and clinical adjudication by doctors. …”
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    Clinical evaluation of metagenomic next-generation sequencing in unbiased pathogen diagnosis of urinary tract infection
  14. 434
    “…If >60 % of sequencing reads are human (7/98 samples) susceptibility prediction becomes compromised. Novel MinION-based WGS (n=9) currently gives rapid species identification but not susceptibility prediction. …”
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    DNA extraction from primary liquid blood cultures for bloodstream infection diagnosis using whole genome sequencing
  15. 435
    “…RESULTS: The MinION run of the HCMV (strain TB40/E) library resulted in ~ 47,000 reads from a single R9 flowcell and in ~ 100× average read depth across the virus genome. …”
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    Nanopore sequencing and full genome de novo assembly of human cytomegalovirus TB40/E reveals clonal diversity and structural variations
  16. 436
    por Baeza, J. Antonio
    Publicado 2020
    “…RESULTS: LC-WGS using a MinION ONT device and various de-novo and reference-based assembly pipelines retrieved a complete and highly accurate mitogenome for the Caribbean spiny lobster Panulirus argus. …”
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    Yes, we can use it: a formal test on the accuracy of low-pass nanopore long-read sequencing for mitophylogenomics and barcoding research using the Caribbean spiny lobster Panulirus argus
  17. 437
    “…Thirteen ALS loci in C. tropicalis strain MYA-3404 were deduced from a genome assembly constructed from Illumina MiSeq and Oxford Nanopore MinION data. Although the MinION data were valuable, PCR amplification and Sanger sequencing of ALS loci were still required to complete and verify the gene sequences. …”
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    Pursuing Advances in DNA Sequencing Technology to Solve a Complex Genomic Jigsaw Puzzle: The Agglutinin-Like Sequence (ALS) Genes of Candida tropicalis
  18. 438
    “…Whole-genome sequencing analysis using MinION revealed that bla(NDM–5) gene was carried on a 93.9-kb plasmid with IncFIA/IncFIB/IncFII/IncQ1 replicons, and bla(OXA–181) gene was carried on a 51.5-kb plasmid with the IncX3 replicon in E. coli isolates. bla(OXA–23) was encoded in two locations on the chromosome of A. baumannii. …”
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    On-Site Genomic Epidemiological Analysis of Antimicrobial-Resistant Bacteria in Cambodia With Portable Laboratory Equipment
  19. 439
    “…In this study, we determined the genome sequence of C. albicans TIMM1768 using reads obtained from portable MinION and benchtop Ion PGM sequencers. Genome annotation and a comparative analysis with published genomes revealed that the TIMM1768 strain was close to Candida albicans CHN1, and we identified a significant number of genes encoding for pathogenesis. …”
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    Utilization of Hybrid Assembly Approach to Determine the Genome of an Opportunistic Pathogenic Fungus, Candida albicans TIMM 1768
  20. 440
    por Baltrus, David A., Clark, Meara
    Publicado 2019
    “…Hybrid assembly strategies that combine long‐read sequencing reads from Oxford Nanopore's MinION device combined with high‐depth Illumina paired‐end reads have enabled completion and circularization of both plasmids and chromosomes from multiple bacterial strains. …”
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    A complete genome sequence for Pseudomonas syringae pv. pisi PP1 highlights the importance of multiple modes of horizontal gene transfer during phytopathogen evolution
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