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441“…In this study, to elucidate the mouse Y chromosome, we enriched the mouse Y chromosome using a fluorescence-activated cell sorter (FACS) equipped with commonly used UV and blue 488 nm lasers and read the nucleotides using the Oxford Nanopore MinION long-read sequencer. This sequencing strategy allows us to cover the whole known region as well as the potential undetermined region of the Y chromosome. …”
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442por Srivastava, Prakhar, Gomathinayagam, Sankaranarayanan, Easwaran, Nalini, Sankar, Gowri, Padmavathi, E., Shankar, Manoharan, Gothandam, Kodiveri M., Sivashanmugam, Karthikeyan“…The whole genome sequencing library was prepared according to the Oxford Nanopore's SQK-LSK108 kit protocol on Oxford Nanopore's Minion platform. The 7.1 Mb and 6.3-Mb draft genome sequence with GC content of 65.8% and 66.4% respectively provides insight into their resistance mechanism and virulence factors.…”
Publicado 2020
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443por Hale, Jon Michael“…A total of 20 students from schools across Jersey are attempting to sequence the chloroplast genomes from daffodils that they have collected from non-cultivated locations using Oxford Nanopore Technologies’ MinION. Despite site closures due to COVID-19 control measures, the project has developed insight into different scientific careers through experience and ownership of the entire project pipeline. …”
Publicado 2020
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444por Torsson, Emeli, Kgotlele, Tebogo, Misinzo, Gerald, Johansson Wensman, Jonas, Berg, Mikael, Karlsson Lindsjö, Oskar“…The use of a portable miniPCR and MinION brings the laboratory to the field and in addition makes the production of a full genome possible within 24 h of sampling. …”
Publicado 2020
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445por Brejová, Broňa, Boršová, Kristína, Hodorová, Viktória, Čabanová, Viktória, Gafurov, Askar, Fričová, Dominika, Neboháčová, Martina, Vinař, Tomáš, Klempa, Boris, Nosek, Jozef“…Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for analysis of the SARS-CoV-2 genome sequences. …”
Publicado 2021
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446“…According to Oxford Nanopore Technologies (ONT), MinION is a compact and portable sequencing device. …”
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447por Quan, Jingjing, Hu, Huangdu, Zhang, Huichuan, Meng, Yan, Liao, Weichao, Zhou, Junxin, Han, Xinhong, Shi, Qiucheng, Zhao, Dongdong, Wang, Qian, Jiang, Yan, Yu, Yunsong“…The complete chromosome and plasmid sequences were obtained by combining Illumina paired-end short reads and MinION long reads. S1-PFGE, southern blot analysis and conjugation assay confirmed the transferability of the mcr-1-harboring plasmid. …”
Publicado 2023
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448por Minion, Lindsey E., Chase, Dana M., Farley, John H., Willmott, Lyndsay J., Monk, Bradley J.Enlace del recurso
Publicado 2016
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449“…We showed that even 40 min of the MinION run was enough for bacterial identification and detecting the first AMR gene. …”
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450por Angarita, Ana M., Short, Vanessa, Goyal, Neera, McLaughlin, Kim, Minion, Lindsey, King, Jacqueline, Bui, Khanh, Abatemarco, Diane, Gannon, MeghanEnlace del recurso
Publicado 2022
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451por He, Xianhong, Xu, Liwei, Dai, Hangdong, Ge, Minxia, Zhu, Jufang, Fu, Hangyu, Zhu, Shuilong, Shao, Jiayu“…METHODS: The antimicrobial susceptibility of E. coli isolate 488 was measured by using the broth microdilution method. The Oxford Nanopore MinION and Illumina NovaSeq 6000 platforms were applied to determine the whole-genome sequence of this isolate. …”
Publicado 2023
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452por Pollo, Stephen M. J., Reiling, Sarah J., Wit, Janneke, Workentine, Matthew L., Guy, Rebecca A., Batoff, G. William, Yee, Janet, Dixon, Brent R., Wasmuth, James D.“…CONCLUSIONS: The ability to generate reference quality genomes from a single MinION run and a multiplexed MiSeq run enables future large-scale comparative genomic studies within the genus Giardia. …”
Publicado 2020
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453por Wongsurawat, Thidathip, Jenjaroenpun, Piroon, Taylor, Mariah K., Lee, Jasper, Tolardo, Aline Lavado, Parvathareddy, Jyothi, Kandel, Sangam, Wadley, Taylor D., Kaewnapan, Bualan, Athipanyasilp, Niracha, Skidmore, Andrew, Chung, Donghoon, Chaimayo, Chutikarn, Whitt, Michael, Kantakamalakul, Wannee, Sutthent, Ruengpung, Horthongkham, Navin, Ussery, David W., Jonsson, Colleen B., Nookaew, Intawat“…Long-read nanopore sequencing by a MinION device offers the unique possibility to directly sequence native RNA. …”
Publicado 2019
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454por Li, Shengjun, Jia, Shangang, Hou, Lili, Nguyen, Hanh, Sato, Shirley, Holding, David, Cahoon, Edgar, Zhang, Chi, Clemente, Tom, Yu, Bin“…The Ds element-enriched DNAs are then sequenced using the MinION-based platform of Nanopore. This method allowed us to rapidly map the Ds insertion positions in 51 transgenic soybean lines through a single sequencing run. …”
Publicado 2019
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455por King, Jacqueline, Schulze, Christoph, Engelhardt, Andreas, Hlinak, Andreas, Lennermann, Sara-Lisa, Rigbers, Kerstin, Skuballa, Jasmin, Staubach, Christoph, Mettenleiter, Thomas C., Harder, Timm, Beer, Martin, Pohlmann, Anne“…Full-genome sequencing was conducted on the MinION platform, enabling further phylogenetic analyses. …”
Publicado 2020
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456por Alpert, Tara, Vogels, Chantal B.F., Breban, Mallery I., Petrone, Mary E., Wyllie, Anne L., Grubaugh, Nathan D., Fauver, Joseph R.“…Using the ARTIC Network amplicon-generation approach with sequencing on the Oxford Nanopore MinION, we demonstrate that sequencing SARS-CoV-2 from saliva produces genomes comparable to those from nasopharyngeal swabs, and that RNA extraction is necessary to generate complete genomes from saliva. …”
Publicado 2021
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457“…The portability of the MinION and Flongle platforms combined with lowering costs and continued improvements in read accuracy make ONT an attractive method for both low- and high-scale virus diagnostics. …”
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458por Boykin, Laura M., Sseruwagi, Peter, Alicai, Titus, Ateka, Elijah, Mohammed, Ibrahim Umar, Stanton, Jo-Ann L., Kayuki, Charles, Mark, Deogratius, Fute, Tarcisius, Erasto, Joel, Bachwenkizi, Hilda, Muga, Brenda, Mumo, Naomi, Mwangi, Jenniffer, Abidrabo, Phillip, Okao-Okuja, Geoffrey, Omuut, Geresemu, Akol, Jacinta, Apio, Hellen B., Osingada, Francis, Kehoe, Monica A., Eccles, David, Savill, Anders, Lamb, Stephen, Kinene, Tonny, Rawle, Christopher B., Muralidhar, Abishek, Mayall, Kirsty, Tairo, Fred, Ndunguru, Joseph“…In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with the MinION and MinIT mobile sequencing devices developed by Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. …”
Publicado 2019
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459por Juma, Meshack, Sankaradoss, Arun, Ndombi, Redcliff, Mwaura, Patrick, Damodar, Tina, Nazir, Junaid, Pandit, Awadhesh, Khurana, Rupsy, Masika, Moses, Chirchir, Ruth, Gachie, John, Krishna, Sudhir, Sowdhamini, Ramanathan, Anzala, Omu, Meenakshi, Iyer S.“…Increasing accuracy of base-calling algorithms, high throughput, error-correction strategies, and ease of using the mobile sequencer MinION in remote areas lead to its adoption for routine microbial genome sequencing. …”
Publicado 2021
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460por Gliddon, Harriet D., Frampton, Dan, Munsamy, Vanisha, Heaney, Jude, Pataillot-Meakin, Thomas, Nastouli, Eleni, Pym, Alexander S., Steyn, Adrie J. C., Pillay, Deenan, McKendry, Rachel A.“…The DNA is then read using a portable sequencer called a MinION, which indicates whether there are tell-tale changes in the DNA that indicate whether the TB strain is drug resistant. …”
Publicado 2021
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