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461por Logan, Robert, Fleischmann, Zoe, Annis, Sofia, Wehe, Amy Wangsness, Tilly, Jonathan L., Woods, Dori C., Khrapko, Konstantin“…For biological ONT MinION data, 3GOLD clustering sensitivity was 27.99% higher than Sequence-Levenstein distance, 52.76% higher than Levenshtein distance, 56.39% higher than Starcode, 48% higher than CD-HIT-EST and 70.4% higher than DNACLUST. …”
Publicado 2022
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462“…Horizontal crabbing is obtained with a radio-frequency dipole cavity (RFD) designed by Old Do-minion University (ODU), SLAC and Fermilab (FNAL). …”
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463por Wilson, Rebecca C., Butters, Oliver W., Clark, Tom, Minion, Joel, Turner, Andrew, Murtagh, Madeleine J.Enlace del recurso
Publicado 2017
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464“…An Oxford Nanopore Technologies-based compact and portable MinION platform was used to identify the causal viral pathogen. …”
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465“…Our setup implemented the MinION mk1B, an NVIDIA Jetson Xavier GPU, and flongle flow cells. …”
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466Rapid diagnosis of Mycobacterium marinum infection using targeted nanopore sequencing: a case report“…However, this method is time-consuming and has low positivity rates for fastidious organisms. Oxford Nanopore MinION sequencing is an emerging third-generation sequencing technology that can sequence DNA or RNA directly in a culture-independent manner and offers rapid microbial identification. …”
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467por Chandler, Jodie, Camberis, Mali, Bouchery, Tiffany, Blaxter, Mark, Le Gros, Graham, Eccles, David A“…We de-novo assembled a single contig mitochondrial genome from N. brasiliensis using MinION R9 nanopore data. The assembly was error-corrected using existing Illumina HiSeq reads, and annotated in full (i.e. gene boundary definitions without substantial gaps) by comparing with annotated genomes from similar parasite relatives. …”
Publicado 2017
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468por Cretu Stancu, Mircea, van Roosmalen, Markus J., Renkens, Ivo, Nieboer, Marleen M., Middelkamp, Sjors, de Ligt, Joep, Pregno, Giulia, Giachino, Daniela, Mandrile, Giorgia, Espejo Valle-Inclan, Jose, Korzelius, Jerome, de Bruijn, Ewart, Cuppen, Edwin, Talkowski, Michael E., Marschall, Tobias, de Ridder, Jeroen, Kloosterman, Wigard P.“…Here we analyse the genomes of two patients with congenital abnormalities using the MinION nanopore sequencer and a novel computational pipeline—NanoSV. …”
Publicado 2017
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469por Kuderna, Lukas F. K., Lizano, Esther, Julià, Eva, Gomez-Garrido, Jessica, Serres-Armero, Aitor, Kuhlwilm, Martin, Alandes, Regina Antoni, Alvarez-Estape, Marina, Juan, David, Simon, Heath, Alioto, Tyler, Gut, Marta, Gut, Ivo, Schierup, Mikkel Heide, Fornas, Oscar, Marques-Bonet, Tomas“…To facilitate the assembly of such complicated genomic territory, we developed a novel strategy to sequence native, unamplified flow sorted DNA on a MinION nanopore sequencing device. Our approach yields a highly continuous assembly of the first human Y chromosome of African origin. …”
Publicado 2019
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470por Arumugam, Krithika, Bağcı, Caner, Bessarab, Irina, Beier, Sina, Buchfink, Benjamin, Górska, Anna, Qiu, Guanglei, Huson, Daniel H., Williams, Rohan B. H.“…RESULTS: We demonstrate that whole bacterial chromosomes can be obtained from an enriched community, by application of MinION sequencing to a sample from an EBPR bioreactor, producing 6 Gb of sequence that assembles into multiple closed bacterial chromosomes. …”
Publicado 2019
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471por Matsumoto, Yuki, Kinjo, Takeshi, Motooka, Daisuke, Nabeya, Daijiro, Jung, Nicolas, Uechi, Kohei, Horii, Toshihiro, Iida, Tetsuya, Fujita, Jiro, Nakamura, Shota“…This method showed the highest sensitivity and specificity amongst conventional methods and demonstrated the capacity for rapid detection of NTM, 10 min of sequencing of the ONT MinION being sufficient. Application of this methodology could improve disease epidemiology and increase the cure rates of NTM diseases.…”
Publicado 2019
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472“…LFastqC is compared with several state-of-the-art compressors, and the results indicate that LFastqC achieves better compression ratios for important datasets such as the LS454, PacBio, and MinION. Moreover, LFastqC has a better compression and decompression speed than LFQC, which was previously the top-performing compression algorithm for the LS454 dataset. …”
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473por Hamidian, Mohammad, Blasco, Lucia, Tillman, Lauren N., To, Joyce, Tomas, María, Myers, Garry S. A.“…Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. …”
Publicado 2020
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474por Wang, Kaiying, Liu, Xiong, Liu, Huiying, Li, Peihan, Lin, Yanfeng, Yin, Dongdong, Yang, Lang, Li, Jinhui, Li, Shenlong, Jia, Leili, Bai, Changqing, Jiang, Yongqiang, Li, Peng, Song, Hongbin“…Diagnosis of C. psittaci pneumonia is often delayed due to nonspecific clinical presentations and limited laboratory diagnostic techniques. RESULTS: The MinION platform established the diagnosis in the shortest time, while BGISEQ-500 generated additional in-depth sequence data that included the rapid characterization of antibiotic susceptibility. …”
Publicado 2021
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475por Alpert, Tara, Vogels, Chantal B F, Breban, Mallery I, Petrone, Mary E, Wyllie, Anne L, Grubaugh, Nathan D, Fauver, Joseph R“…Using the ARTIC Network amplicon-generation approach with sequencing on the Oxford Nanopore MinION, we demonstrate that sequencing SARS-CoV-2 from saliva produces genomes comparable to those from NP swabs, and that RNA extraction is necessary to generate complete genomes from saliva. …”
Publicado 2022
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476“…These were identified using the MinION Oxford Nanopore system and Kirby–Bauer disc diffusion assays to examine the bacterial responses to antibiotic exposure. …”
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477por Andersen, P., Barksdale, S., Barclay, R.A., Smith, N., Fernandes, J., Besse, K., Goldfarb, D., Barbero, R., Dunlap, R., Jones-Roe, T., Kelly, R., Miao, S., Ruhunusiri, C., Munns, A., Mosavi, S., Sanson, L., Munns, D., Sahoo, S., Swahn, O., Hull, K., White, D., Kolb, K., Noroozi, F., Seelam, J., Patnaik, A., Lepene, B.“…Presented here is a magnetic hydrogel particle enabled workflow for capturing and concentrating SARS-CoV-2 from diagnostic remnant swab samples that significantly improves sequencing results using the Oxford Nanopore Technologies MinION sequencing platform. Our approach utilizes a novel affinity-based magnetic hydrogel particle, circumventing low input sample volumes and allowing for both rapid manual and automated high throughput workflows that are compatible with Nanopore sequencing. …”
Publicado 2023
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478“…More recently, a new fourth generation of Nanopore based single molecule sequencing technology, was developed based on MinION(®) sequencer which is portable, inexpensive and fast. …”
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479“…Here we demonstrate that the MinION nanopore sequencer is capable of producing very long reads to resolve both variants and haplotypes of HLA-A, HLA-B and CYP2D6 genes important in determining patient drug response in sample NA12878 of CEPH/UTAH pedigree 1463, without the need for statistical phasing. …”
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480por Li, Chenhao, Chng, Kern Rei, Boey, Esther Jia Hui, Ng, Amanda Hui Qi, Wilm, Andreas, Nagarajan, Niranjan“…CONCLUSIONS: INC-Seq reads enabled accurate species-level classification, identification of species at 0.1 % abundance and robust quantification of relative abundances, providing a cheap and effective approach for pathogen detection and microbiome profiling on the MinION system. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13742-016-0140-7) contains supplementary material, which is available to authorized users.…”
Publicado 2016
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