Mostrando 561 - 580 Resultados de 989 Para Buscar '"Minions"', tiempo de consulta: 0.32s Limitar resultados
  1. 561
    “…Here, we compared seven platforms, encompassing second generation sequencers (Illumina HiSeq 300, MGI DNBSEQ-G400 and DNBSEQ-T7, ThermoFisher Ion GeneStudio S5 and Ion Proton P1) and third generation sequencers (Oxford Nanopore Technologies MinION R9 and Pacific Biosciences Sequel II). We constructed three uneven synthetic microbial communities composed of up to 87 genomic microbial strains DNAs per mock, spanning 29 bacterial and archaeal phyla, and representing the most complex and diverse synthetic communities used for sequencing technology comparisons. …”
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    Benchmarking second and third-generation sequencing platforms for microbial metagenomics
  2. 562
    “…We cut five 50-kbp tiled sub-regions of Midas genomic DNA using Cas9, followed by sequencing on a MinION device and de novo assembly, generating a single contig spanning the whole 250-kbp region. …”
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    CRISPR/Cas9-Mediated Enrichment Coupled to Nanopore Sequencing Provides a Valuable Tool for the Precise Reconstruction of Large Genomic Target Regions
  3. 563
    “…We present a de novo assembled draft genome and functional annotation for D. arcuata, using a combination of short and long sequencing reads generated by Illumina HiSeq X and Oxford Nanopore Technologies (ONT) MinION sequencing platforms, respectively. A total of 460,694,612 150bp paired-end Illumina and 395,890 ONT raw reads were assembled into 11,493 scaffolds spanning a genome size of 270.5Mb. …”
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    Draft genome assembly and annotation of the masked birch caterpillar, Drepana arcuata (Lepidoptera: Drepanoidea)
  4. 564
    “…DNA from four sweet cherry seedlings derived from gamma-irradiated female parents was sequenced via nanopore technology (Oxford Nanopore MinION). Total data yield was 8.07 Gb, ranging from 0.92 to 3.36 Gb per sample, with the average length of mapped reads ranging from 22 Kbp–24 Kbp. …”
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    Nanopore sequencing data and structural variants identified in Prunus avium seedlings derived through mutagenesis
  5. 565
    “…MATERIALS AND METHODS: We collected ticks, extracted, and sequenced RNA, using Illumina (NovaSeq 6000) and Oxford Nanopore (MinION). RESULTS: From the total generated sequencing reads, 180,559 (~0.35%) and 197,801 (~0.34%) reads were identified as virus-related reads in male and female tick samples, respectively. …”
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    Virome diversity of Hyalomma dromedarii ticks collected from camels in the United Arab Emirates
  6. 566
    “…The mRNA libraries were prepared, and RNA-Sequencing was conducted using the PCR-cDNA approach through the MinION sequencing platform. Our results demonstrated that BSFL reared on BSG and WH had the highest abundance of Bacteroides and Dysgonomonas. …”
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    Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
  7. 567
    “…[Image: see text] We describe a method for direct tRNA sequencing using the Oxford Nanopore MinION. The principal technical advance is custom adapters that facilitate end-to-end sequencing of individual transfer RNA (tRNA) molecules at subnanometer precision. …”
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    Direct Nanopore Sequencing of Individual Full Length tRNA Strands
  8. 568
    “…We sequenced the gut DNA with Oxford Nanopore Technologies’ (ONT) MinION sequencer, then analyzed the data using MEGAN-LR, Kraken2 protein mode, Kraken2 nucleotide mode, GraphMap, and Minimap2 to classify these long ONT reads. …”
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    Benchmarking assembly free nanopore read mappers to classify complex millipede gut microbiota via Oxford Nanopore Sequencing Technology
  9. 569
    “…In this study, we investigated a sample originating from a mute swan that died in November 2021 in the northern part of Bosnia and Herzegovina with RT-qPCR and whole genome sequencing using ONT MinION. It was diagnosed as a highly pathogenic Influenza A virus, subtype H5N1 of 2.3.4.4b clade, and phylogenetic analysis revealed high sequence homology with other European HPAI H5N1 sequences at the time. …”
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    Molecular characterization and phylogenetic analysis of highly pathogenic H5N1 clade 2.3.4.4b virus in Bosnia and Herzegovina
  10. 570
    “…We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus (EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore sequencing coupled to a newly developed, web-based pipeline for real-time bioinformatics analysis on a computational server or laptop (MetaPORE). …”
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    Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
  11. 571
    “…Recently, a nanopore-based sequencing instrument, the Oxford Nanopore MinION, has become available, and we used this for sequencing the Saccharomyces cerevisiae genome. …”
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    Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome
  12. 572
    “…Here, we employed cDNA amplicon sequencing using a long-read portable sequencer, MinION, to characterize various types of mutations in cancer-related genes, namely, EGFR, KRAS, NRAS and NF1. …”
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    Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer
  13. 573
    “…In this study, we used long-read sequencing for the analysis of pseudorabies virus (PRV) transcriptome, including Oxford Nanopore Technologies MinION, PacBio RS-II, and Illumina HiScanSQ platforms. …”
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    Multi-Platform Sequencing Approach Reveals a Novel Transcriptome Profile in Pseudorabies Virus
  14. 574
    “…We report the sequencing and assembly of a reference genome for the human GM12878 Utah/Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore sequencer. 91.2 Gb of sequence data, representing ∼30× theoretical coverage, were produced. …”
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    Nanopore sequencing and assembly of a human genome with ultra-long reads
  15. 575
    “…The Oxford Nanopore’s MinION device generated 44,006 reads using their regular cDNA-sequencing method, whereas 29,832 and 120,394 reads were produced by using the direct RNA-sequencing and the Cap-selection protocols, respectively. …”
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    Transcriptome-wide survey of pseudorabies virus using next- and third-generation sequencing platforms
  16. 576
    “…We designed an adapter to short highly conserved termini of the influenza A virus genome to target the (-) sense RNA into a protein nanopore on the Oxford Nanopore MinION sequencing platform. Utilizing this method with total RNA extracted from the allantoic fluid of influenza rA/Puerto Rico/8/1934 (H1N1) virus infected chicken eggs (EID(50) 6.8 × 10(9)), we demonstrate successful sequencing of the coding complete influenza A virus genome with 100% nucleotide coverage, 99% consensus identity, and 99% of reads mapped to influenza A virus. …”
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    Direct RNA Sequencing of the Coding Complete Influenza A Virus Genome
  17. 577
    “…A metagenomic analysis was carried out to identify potential pathogens in the river water. The Oxford Nanopore MinION platform was used to sequence DNA in near real time to identify both uncultured and a coliform-enriched culture of microbes collected from a popular summer swimming area of the Little Bighorn River. …”
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    Metagenomic Profiling of Microbial Pathogens in the Little Bighorn River, Montana
  18. 578
    “…Here we describe direct MinION nanopore sequencing of individual, full-length 16S rRNA absent reverse transcription or amplification. …”
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    Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing
  19. 579
    “…Here, we report de novo assemblies, annotation, and comparative analyses of the Berenice strain using a combination of Illumina short reads and MinION long reads. Our work demonstrates that Nanopore sequencing improves T. cruzi assembly contiguity and increases the assembly size in ∼16 Mb. …”
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    Nanopore Sequencing Significantly Improves Genome Assembly of the Protozoan Parasite Trypanosoma cruzi
  20. 580
    “…Robust single-nucleotide polymorphism typing using MinION-based variant calling is possible, and we provide evidence that the 2 technologies can be used interchangeably to type STEC O157:H7 in a public health setting.…”
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    Comparison of single-nucleotide variants identified by Illumina and Oxford Nanopore technologies in the context of a potential outbreak of Shiga toxin–producing Escherichia coli
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