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41“…In this study, two types of soil were prepared experimentally to investigate the possibility of simultaneously analyzing multiple environmental samples using MinION. The MinION sequencing of amplicons was adjusted by the different rounds of PCR performed. …”
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42“…PURPOSE OF REVIEW: The introduction of MinION whole-genome sequencing technology greatly increased and simplified complete genome sequencing in various fields of science across the globe. …”
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43por Sato, Priscila de Morais, Leite, Fernanda Helena Marrocos, Khandpur, Neha, Martins, Ana Paula Bortoletto, Mais, Laís AmaralEnlace del recurso
Publicado 2022
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44por Miah, Mojnu, Hossain, Mohammad Enayet, Hasan, Rashedul, Alam, Md Shaheen, Puspo, Joynob Akter, Hasan, Md Mahmudul, Islam, Ariful, Chowdhury, Sukanta, Rahman, Mohammed Ziaur“…First, the library was prepared using the ligation sequencing kit, barcoded individually using the native barcodes, and sequenced on the MinION MK 1C platform with real-time base-calling. …”
Publicado 2023
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45por de Souza, Leandro Menezes, de Oliveira, Isabelle Dias, Sales, Flávia Cristina Silva, da Costa, Antonio Charlys, Campos, Karoline Rodrigues, Abbud, Adriano, Guerra, Juliana Mariotti, dos Santos Cirqueira Borges, Cinthya, Takahashi, Carlos Pires Fernandes Júnior, de Araújo, Leonardo José Tadeu“…Among the metrics studied, running time, read production, and Q score were better represented in Illumina sequencing, while the MinIOn platform showed better response time and greater diversity of generated files. …”
Publicado 2023
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46por Judge, Kim, Harris, Simon R., Reuter, Sandra, Parkhill, Julian, Peacock, Sharon J.“…RESULTS: An evaluation of the accuracy of the MinION based on sequencing of an MRSA isolate showed that error rates were higher in the MinION reads, but provided an even coverage across the entire genome length. …”
Publicado 2015
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47“…MinION is a memory stick–sized nanopore-based sequencer designed primarily for single-molecule sequencing of long DNA fragments (>6 kb). …”
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48por Judge, Kim, Hunt, Martin, Reuter, Sandra, Tracey, Alan, Quail, Michael A., Parkhill, Julian, Peacock, Sharon J.“…Three software tools (PBcR, Canu and miniasm) were used to assemble MinION data and a fourth (SPAdes) was used to combine MinION and Illumina data to produce a hybrid assembly. …”
Publicado 2016
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49“…The nature of the MinION output requires new tools that are easy to use by scientists with a range of computational skills and which enable quick and simple QC and data extraction from MinION runs. …”
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50Fusobacterium Genomics Using MinION and Illumina Sequencing Enables Genome Completion and Correction“…Here we report the first eight complete Fusobacterium genomes sequenced using an Oxford Nanopore MinION and Illumina sequencing pipeline and assembled using the open-source program Unicycler. …”
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51“…RESULTS: We sequenced symbiotic algae Chlorella variabilis using Oxfofrd Nanopore MinION as the long-read sequencer and Illumina HiSeq 4000 as the short-read sequencer and then assembled the genomes under various conditions. …”
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52por Xu, Yifei, Lewandowski, Kuiama, Lumley, Sheila, Pullan, Steven, Vipond, Richard, Carroll, Miles, Foster, Dona, Matthews, Philippa C., Peto, Timothy, Crook, Derrick“…Metagenomic sequencing with the Oxford Nanopore MinION sequencer offers potential for point-of-care testing of infectious diseases in clinical settings. …”
Publicado 2018
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53por Peserico, Alessia, Marcacci, Maurilia, Malatesta, Daniela, Di Domenico, Marco, Pratelli, Annamaria, Mangone, Iolanda, D’Alterio, Nicola, Pizzurro, Federica, Cirone, Francesco, Zaccaria, Guendalina, Cammà, Cesare, Lorusso, Alessio“…In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. …”
Publicado 2019
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54por Shabardina, Victoria, Kischka, Tabea, Manske, Felix, Grundmann, Norbert, Frith, Martin C, Suzuki, Yutaka, Makałowski, Wojciech“…Moreover, for the latter group, we describe the basic algorithm necessary for MinION sequencing analysis from the first to last step.…”
Publicado 2019
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55por Maestri, Simone, Cosentino, Emanuela, Paterno, Marta, Freitag, Hendrik, Garces, Jhoana M., Marcolungo, Luca, Alfano, Massimiliano, Njunjić, Iva, Schilthuizen, Menno, Slik, Ferry, Menegon, Michele, Rossato, Marzia, Delledonne, Massimo“…Barcodes are sequenced using MinION technology and analyzed with ONTrack, an original de novo assembly pipeline that requires as few as 1000 reads per sample. …”
Publicado 2019
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56por Seah, Adeline, Lim, Marisa C.W., McAloose, Denise, Prost, Stefan, Seimon, Tracie A.“…Sequencing errors occurred primarily in homopolymer regions, as identified in previous MinION studies. We demonstrate that it is possible to generate accurate DNA barcode sequences from preserved and non-invasively collected wildlife samples using portable MinION sequencing, creating more opportunities to apply portable sequencing technology for species identification.…”
Publicado 2020
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57por Neal-McKinney, Jason M., Liu, Kun C., Lock, Christopher M., Wu, Wen-Hsin, Hu, Jinxin“…Assemblies generated only from MinION data using Canu were the least accurate, containing many indels and substitutions that affected downstream analyses. …”
Publicado 2021
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58por Haveman, Natasha J., Khodadad, Christina L. M., Dixit, Anirudha R., Louyakis, Artemis S., Massa, Gioia D., Venkateswaran, Kasthuri, Foster, Jamie S.“…Microbial transcripts enriched from host–microbe total RNA were sequenced using the Oxford Nanopore MinION sequencing platform. Results show that this enrichment approach was highly reproducible and could be an effective approach for the on-site detection of microbial transcriptional activity. …”
Publicado 2021
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59por Massaiu, Ilaria, Songia, Paola, Chiesa, Mattia, Valerio, Vincenza, Moschetta, Donato, Alfieri, Valentina, Myasoedova, Veronika A., Schmid, Michael, Cassetta, Luca, Colombo, Gualtiero I., D’Alessandra, Yuri, Poggio, Paolo“…This work aimed to provide the user with the number of reads that should be sequenced, through the ONT MinION platform, to reach the desired accuracy level for a human cell RNA study. …”
Publicado 2021
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60por SHINOZUKA, Yasunori, KAWAI, Kazuhiro, KURUMISAWA, Tomomi, SHIMIZU, Yuko, IMANISHI, Tadashi, OHNO, Ayumu, TAKAHASHI, Mano, KANEKO, Sohei, SUZUKI, Naoki“…We investigated 48 milk samples from 22 cows with no systemic or local clinical signs using MinION(TM) nanopore sequencing for a 16S rRNA gene amplicon. …”
Publicado 2021
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