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641por Hohl, Sarah D., Bird, Jennifer E., Nguyen, Claire V. T., D’Angelo, Heather, Minion, Mara, Pauk, Danielle, Adsit, Robert T., Fiore, Michael, Nolan, Margaret B., Rolland, BetsyEnlace del recurso
Publicado 2022
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642por Gill, Stephanie P., Snelling, William J., Dooley, James S. G., Ternan, Nigel G., Banat, Ibrahim M., Arnscheidt, Joerg, Hunter, William R.“…We then extracted DNA and carried out shotgun sequencing using a MinION portable sequencer to determine microbial community composition, with 16S rRNA analyses (64,678 classifiable reads identified), and AMR gene occurrence (81 instances of AMR genes over 9 AMR gene classes) through a metagenomic analysis. …”
Publicado 2023
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643por Uppili, Bharathram, Sharma, Pooja, Ahmad, Istaq, Sahni, Shweta, Asokachandran, Vivekanand, Nagaraja, Anil B, Srivastava, Achal K, Faruq, Mohammed“…We utilized an approach of long-range targeted amplification of FXN-GAA repeats using Oxford Nanopore Technologies MinION platform for accurate estimation of repeat length. …”
Publicado 2023
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644Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukrainepor Klink, Amy C., Rula, Oleksandr, Sushko, Mykola, Bezymennyi, Maksym, Mezinov, Oleksandr, Gaidash, Oleksandr, Bai, Xiao, Stegniy, Anton, Sapachova, Maryna, Datsenko, Roman, Skorokhod, Sergiy, Nedosekov, Vitalii, Hill, Nichola J., Ninua, Levan, Kovalenko, Ganna, Ducluzeau, Anne Lise, Mezhenskyi, Andriy, Buttler, Jeremy, Drown, Devin M., Causey, Douglas, Stegniy, Borys, Gerilovych, Anton, Bortz, Eric, Muzyka, Denys“…To build One Health’s capacity to characterize APMV virulence and analyze the potential risks of spillover to immunologically naïve populations, we sequenced virus genomes in veterinary research labs in Ukraine using a nanopore (MinION) platform. RNA was extracted and amplified using a multiplex tiling primer approach to specifically capture full-length APMV-1 (n = 5) and APMV-6 (n = 2) genomes at high read depth. …”
Publicado 2023
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645por Flack, Nicole, Drown, Melissa, Walls, Carrie, Pratte, Jay, McLain, Adam, Faulk, Christopher“…All assembly drafts were generated from 158 Gb of sequence using seven minION flow cells.…”
Publicado 2023
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646por Fukushima, Kiyoharu, Matsumoto, Yuki, Matsuki, Takanori, Saito, Haruko, Motooka, Daisuke, Komukai, Sho, Fukui, Eriko, Yamuchi, June, Nitta, Tadayoshi, Niitsu, Takayuki, Abe, Yuko, Nabeshima, Hiroshi, Nagahama, Yasuharu, Nii, Takuro, Tsujino, Kazuyuki, Miki, Keisuke, Kitada, Seigo, Kumanogoh, Atsushi, Akira, Shizuo, Nakamura, Shota, Kida, Hiroshi“…In total, 138 patients were prospectively enrolled between April 2021 and May 2022, and culture-positive MGIT broths were subjected to sequencing using MinION, a portable next-generation sequencer. Sequence analysis was conducted to identify species using core genome multilocus sequence typing and to predict macrolide and amikacin (AMK) resistance based on previously reported mutations in rrl, rrs, and erm(41). …”
Publicado 2023
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647por Sittikul, Pichamon, Batty, Elizabeth M., Yodsawat, Prasert, Nuanpirom, Jiratchaya, Kosoltanapiwat, Nathamon, Sangket, Unitsa, Chatchen, Supawat, Day, Nicholas P. J., Thaipadungpanit, Janjira“…Random amplification-based sequencing using MinION (Oxford Nanopore Technology) helped identify viral transmission between two closely related clones. …”
Publicado 2023
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648por Park, Kyungmin, Noh, Juyoung, Kim, Kijin, Kim, Jongwoo, Cho, Hee-Kyung, Kim, Seong-Gyu, Yang, Eunyoung, Kim, Won-Keun, Song, Jin-Won“…We evaluated the deployable MinION system to obtain high-fidelity entire-length sequences of SEOV for the genome identification of accurate infectious sources and their genetic diversity. …”
Publicado 2023
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649por McIntyre, Alexa B R, Rizzardi, Lindsay, Yu, Angela M, Alexander, Noah, Rosen, Gail L, Botkin, Douglas J, Stahl, Sarah E, John, Kristen K, Castro-Wallace, Sarah L, McGrath, Ken, Burton, Aaron S, Feinberg, Andrew P, Mason, Christopher E“…As a first step toward sequencing in space and aboard the International Space Station (ISS), we tested the Oxford Nanopore Technologies MinION during a parabolic flight to understand the effects of variable gravity on the instrument and data. …”
Publicado 2016
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650por Salazar, Alex N., Gorter de Vries, Arthur R., van den Broek, Marcel, Wijsman, Melanie, de la Torre Cortés, Pilar, Brickwedde, Anja, Brouwers, Nick, Daran, Jean-Marc G., Abeel, Thomas“…In this study, we obtained a near-complete genome assembly of CEN.PK113–7D using only Oxford Nanopore Technology's MinION sequencing platform. Fifteen of the 16 chromosomes, the mitochondrial genome and the 2-μm plasmid are assembled in single contigs and all but one chromosome starts or ends in a telomere repeat. …”
Publicado 2017
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651por Wang, Yue, Lu, Ji, Mao, Likai, Li, Jie, Yuan, Zhiguo, Bond, Philip L., Guo, Jianhua“…The underlying mechanisms of the enhanced HGT were revealed by detecting oxidative stress and cell membrane permeability, in combination with MinION DNA sequencing, genome-wide RNA sequencing, and proteomic analysis. …”
Publicado 2018
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652“…RESULTS: We re-sequenced the H. gammarus mitogenome on an Oxford Nanopore Minion flowcell and performed a long-read only assembly, generating a complete mitogenome assembly for H. gammarus. …”
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653“…A total of ∼4,480 colonies were screened for the presence of cellular BChl a, resulting in 129 AAP isolates that were further clustered into 21 groups based on MALDI-TOF MS profiling, representatives of which were sequenced using the Illumina NextSeq and Oxford Nanopore MinION platforms. Seventeen draft and four complete genomes of AAPs were assembled belonging in Methylobacterium, Rhizobium, Roseomonas, and a novel Alsobacter. …”
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654por Taylor, Tonya L., Volkening, Jeremy D., DeJesus, Eric, Simmons, Mustafa, Dimitrov, Kiril M., Tillman, Glenn E., Suarez, David L., Afonso, Claudio L.“…In sequencing runs as short as four hours, using the MinION instrument, we obtained full-length genomes with an average identity of 99.87% for Salmonella Bareilly and 99.89% for E. coli in comparison to the respective MiSeq references. …”
Publicado 2019
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655por Baloch, Zulqarnain, Lv, Luchao, Yi, Lingxian, Wan, Miao, Aslam, Bilal, Yang, Jun, Liu, Jian-Hua“…Whole-genome sequencing and Oxford Nanopore MinION sequencing revealed that 15978 and C4109 belonged to ST156 and ST167, respectively. bla(NDM-7) was carried by an IncX3 plasmid that has disseminated worldwide, whereas bla(NDM-5) was located on an F36: A-: B32 plasmid, which shared high identity with two plasmids carried by E. coli isolates from other countries (one from a patient in Canada). …”
Publicado 2019
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656por Burton, Aaron S., Stahl, Sarah E., John, Kristen K., Jain, Miten, Juul, Sissel, Turner, Daniel J., Harrington, Eoghan D., Stoddart, David, Paten, Benedict, Akeson, Mark, Castro-Wallace, Sarah L.“…The MinION sequencer has made in situ sequencing feasible in remote locations. …”
Publicado 2020
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657por Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M, Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne-Lise, Leggett, Richard M, Heavens, Darren, Peel, Ned, Snutch, Terrance P, Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R, Jain, Miten, Brown, Bonnie L“…RESULTS: We investigated the metagenomes of 11 rivers across 3 continents using MinION nanopore sequencing, a portable platform that could be useful for future global river monitoring. …”
Publicado 2020
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658por Kumar, Pramod, Pandey, Rajesh, Sharma, Pooja, Dhar, Mahesh S., A., Vivekanand, Uppili, Bharathram, Vashisht, Himanshu, Wadhwa, Saruchi, Tyagi, Nishu, Fatihi, Saman, Sharma, Uma, Singh, Priyanka, Lall, Hemlata, Datta, Meena, Gupta, Poonam, Saini, Nidhi, Tewari, Aarti, Nandi, Bibhash, Kumar, Dhirendra, Bag, Satyabrata, Gahlot, Deepanshi, Rathore, Surabhi, Jatana, Nidhi, Jaiswal, Varun, Gogia, Hema, Madan, Preeti, Singh, Simrita, Singh, Prateek, Dash, Debasis, Bala, Manju, Kabra, Sandhya, Singh, Sujeet, Mukerji, Mitali, Thukral, Lipi, Faruq, Mohammed, Agrawal, Anurag, Rakshit, Partha“…Methods: We used ARTIC protocol-based tiling amplicon sequencing of SARS-CoV-2 (n=104) from different states of India using a combination of MinION and MinIT sequencing from Oxford Nanopore Technology to understand how introduction and local transmission occurred. …”
Publicado 2020
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659por de Jong, Samuel I., van den Broek, Marcel A., Merkel, Alexander Y., de la Torre Cortes, Pilar, Kalamorz, Falk, Cook, Gregory M., van Loosdrecht, Mark C. M., McMillan, Duncan G. G.“…To address this, Oxford Nanopore Technology’s MinION allowed us to span these repeats through long reads, with over 6000-fold coverage. …”
Publicado 2020
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660por Fenlon, Shannon N., Chee, Yuemin Celina, Chee, Jacqueline Lai Yuen, Choy, Yeen Hui, Khng, Alexis Jiaying, Liow, Lu Ting, Mehershahi, Kurosh S., Ruan, Xiaoan, Turner, Stephen W., Yao, Fei, Chen, Swaine L.“…We now provide matched whole genome sequencing data generated using the PacBio RSII, Oxford Nanopore MinION R9.4, Ion Torrent, ABI SOLiD, and Illumina NextSeq sequencers. …”
Publicado 2020
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