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681“…The DNA was sequenced using Illumina Novaseq 6000 (Novogen AIT, Singapore) for short-reads data, and MinION of Nanopore following manufacture’s protocols SQK-LSK110 for long-reads data. …”
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682por Peng, Zhong, Hu, Zizhe, Li, Zugang, Li, Xiaosong, Jia, Chaoying, Zhang, Xiaoxue, Wu, Bin, Chen, Huanchun, Wang, Xiangru“…Antimicrobial susceptibility testing showed that HeN100 was resistant to three aminoglycosides, twelve β-lactams including three carbapenems, one phenicol, two tetracyclines, two fluoroquinolones, nitrofurantoin, and colistin tested. Oxford Nanopore MinION sequencing revealed that the complete genomes of the multidrug resistant (MDR) HeN100 consisted of a single circular chromosome and five circular plasmids. …”
Publicado 2019
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683“…RNA was isolated with Trizol from 5 day old protonemata and sequenced using kit SQK-RNA002, flow cells FLO-MIN106 and a MinION device (Oxford Nanopore Technologies Ltd., UK (ONT)) in three biological repeats. …”
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684por Baseggio, Laura, Rudenko, Oleksandra, Buller, Nicky, Landos, Matt, Englestädter, Jan, Barnes, Andrew C.“…Using a combination of Oxford Nanopore MinION long reads and short Illumina reads, we fully sequenced, closed and curated the genomes of two strains of a primary aquatic pathogen Photobacterium damselae subsp. piscicida isolated in Australia. …”
Publicado 2021
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685por Jacob, Jobin John, Pragasam, Agila Kumari, Vasudevan, Karthick, Veeraraghavan, Balaji, Kang, Gagandeep, John, Jacob, Nagvekar, Vasant, Mutreja, Ankur“…Two of five isolates were additionally sequenced using Oxford Nanopore MinION to completely characterize the plasmid and understand its transmission dynamics within Enterobacteriaceae. …”
Publicado 2021
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686por Egorova, Anna, Mikhaylova, Yulia, Saenko, Stepan, Tyumentseva, Marina, Tyumentsev, Aleksandr, Karbyshev, Konstantin, Chernyshkov, Aleksey, Manzeniuk, Igor, Akimkin, Vasiliy, Shelenkov, Andrey“…In this study, we present the first detailed genomic analysis report based on short-read (Illumina) whole-genome sequencing (WGS) of 45 multidrug-resistant (MDR) Salmonella enterica subsp. enterica serotype Infantis isolates from poultry and meat product samples obtained in Russia during 2018–2020, and long-read (MinION) WGS of five more representative isolates. …”
Publicado 2021
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687por Artuso, Irene, Lucidi, Massimiliano, Visaggio, Daniela, Capecchi, Giulia, Lugli, Gabriele Andrea, Ventura, Marco, Visca, Paolo“…By combining Illumina MiSeq, MinION and Sanger technologies, we generated a high-quality whole-genome sequence of A. baumannii ATCC 19606(T), then performed a comparative genome analysis between A. baumannii ATCC 19606(T) strains from several research laboratories and a reference collection. …”
Publicado 2022
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688por Bharat, Amrita, Mataseje, Laura, Parmley, E. Jane, Avery, Brent P., Cox, Graham, Carson, Carolee A., Irwin, Rebecca J., Deckert, Anne E., Daignault, Danielle, Alexander, David C., Allen, Vanessa, El Bailey, Sameh, Bekal, Sadjia, German, Greg J., Haldane, David, Hoang, Linda, Chui, Linda, Minion, Jessica, Zahariadis, George, Reid-Smith, Richard J., Mulvey, Michael R.Enlace del recurso
Publicado 2022
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689por Nowlan, Joseph P., Sies, Ashton N., Britney, Scott R., Cameron, Andrew D. S., Siah, Ahmed, Lumsden, John S., Russell, Spencer“…Ten isolates representing four known and two unknown species of Tenacibaculum were selected for shotgun whole genome sequencing using the Oxford Nanopore’s MinION platform. The genome assemblies achieved closed circular chromosomes for seven isolates and long contigs for the remaining three isolates. …”
Publicado 2023
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690por Scala, Valeria, Faino, Luigi, Costantini, Francesca, Crosara, Valeria, Albanese, Alessio, Pucci, Nicoletta, Reverberi, Massimo, Loreti, Stefania“…In this study, several Italian isolates of Pss recovered in 2015 and 2018 have been characterized by molecular, biochemical, and pathogenicity tests; moreover, their genomes have been assembled through MinION and Illumina–sequencing procedures. Genomic analysis reveals multiple introgression events. …”
Publicado 2023
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691por McClinton, Benjamin, Crinnion, Laura A., McKibbin, Martin, Mukherjee, Rajarshi, Poulter, James A., Smith, Claire E. L., Ali, Manir, Watson, Christopher M., Inglehearn, Chris F., Toomes, Carmel“…Sequencing was performed on MinION flongle flowcells. The resulting fast5 files were basecalled using Guppy, trimmed using Porechop and aligned using Minimap2. …”
Publicado 2023
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692por Deshpande, Lalitagauri M, Vega, Silvio, Tinoco, Juan Carlos, Castanheira, Mariana“…WGS was performed using MiSeq (Illumina) and MinION (Oxford Nanopore). Assembled contigs from short and long reads were combined for in silico screening of resistance genes, MLST, core genome (cg)MLST and SNP analysis. …”
Publicado 2023
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693por Carter, Cailean, Hutchison, Alexandra, Rudder, Steven, Trotter, Elizabeth, Waters, Emma V, Elumogo, Ngozi, Langridge, Gemma C“…These were whole-genome sequenced using the Illumina and MinION platforms for in silico MLST and antibiotic resistance determinant detection. …”
Publicado 2023
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694por Gand, Mathieu, Bloemen, Bram, Vanneste, Kevin, Roosens, Nancy H. C., De Keersmaecker, Sigrid C. J.“…In addition to the comparison of the quality, quantity and purity of the extracted DNA, the performance obtained when doing Nanopore sequencing on a MinION flow cell was also tested. From the obtained results, the Quick-DNA HMW MagBead Kit (Zymo Research) was selected as producing the best yield of pure HMW DNA. …”
Publicado 2023
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695por Graaf-Rau, Annika, Hennig, Christin, Lillie-Jaschniski, Kathrin, Koechling, Monika, Stadler, Julia, Boehmer, Jan, Ripp, Ulrike, Pohlmann, Anne, Schwarz, Bernd-Andreas, Beer, Martin, Harder, Timm“…For passive surveillance, a set of multiplex quantitative reverse transcription real-time PCRs (mRT-qPCR) and MinION-directed sequencing was updated and deployed. …”
Publicado 2023
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696por Hendrix, Jo, Epperson, L. Elaine, Tong, Eric I., Chan, Yvonne L., Hasan, Nabeeh A., Dawrs, Stephanie N., Norton, Grant J., Virdi, Ravleen, Crooks, James L., Chan, Edward D., Honda, Jennifer R., Strong, Michael“…Here, these community scientists were invited for the first time into a high school lab in O’ahu for a genomic sequencing workshop, where participants sequenced four of the collected isolate genomic samples using the Oxford Nanopore Technologies MinION sequencer. Participants generated high quality long read data that when combined with short read Illumina data yielded complete bacterial genomic assemblies suitable for in-depth analysis. …”
Publicado 2023
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697por Ton, Kim N. T., Cree, Simone L., Gronert-Sum, Sabine J., Merriman, Tony R., Stamp, Lisa K., Kennedy, Martin A.“…Here, we describe an approach to HLA-B typing using next generation sequencing (NGS) on the MinION™ nanopore sequencer, combined with data analysis with the SeqNext-HLA software package. …”
Publicado 2018
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698por Tombácz, Dóra, Prazsák, István, Szűcs, Attila, Dénes, Béla, Snyder, Michael, Boldogkői, Zsolt“…In this study, we applied the Pacific Biosciences RSII and Sequel platforms, which altogether resulted in 937,531 mapped reads of inserts (1.42 Gb), while we obtained 2,160,348 aligned reads (1.75 Gb) from the different library preparation methods using the MinION device from Oxford Nanopore Technologies. CONCLUSIONS: By applying cutting-edge technologies, we were able to generate a large dataset that can serve as a valuable resource for the investigation of the dynamic VACV transcriptome, the virus-host interactions, and RNA base modifications. …”
Publicado 2018
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699por Sheahan, Taylor, Hakstol, Rhys, Kailasam, Senthilkumar, Glaister, Graeme D., Hudson, Andrew J., Wieden, Hans-Joachim“…As standard culturing techniques require a laboratory setting, and are time consuming and labour intensive, our approach was designed to be portable, inexpensive and easy to use based on the MinION third-generation sequencing platform from Oxford Nanopore Technologies. …”
Publicado 2019
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700por Dong, Dandan, Li, Manli, Liu, Zhenzhen, Feng, Jiantao, Jia, Nan, Zhao, Hui, Zhao, Baohua, Zhou, Tingting, Zhang, Xianglilan, Tong, Yigang, Zhu, Yuanqi“…The pHFK418-NDM plasmid was sequenced using a hybrid approach based on Illumina and MinION platforms. The sequence of pHFK418-NDM was compared with those of the six other pPrY2001-like plasmids deposited in GenBank. …”
Publicado 2019
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