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701por Nakagawa, So, Inoue, Shigeaki, Kryukov, Kirill, Yamagishi, Junya, Ohno, Ayumu, Hayashida, Kyoko, Nakazwe, Ruth, Kalumbi, Mox, Mwenya, Darlington, Asami, Nana, Sugimoto, Chihiro, Mutengo, Mable M, Imanishi, Tadashi“…Our system comprises of a nanopore technology‐based sequencer, MinION, and two laptop computers. To examine the accuracy and time efficiency of our system, we provided a proof‐of‐concept for the detection of the causative bacteria of 11 meningitis patients in Zambia. …”
Publicado 2019
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702por Salazar, Alex N., Gorter de Vries, Arthur R., van den Broek, Marcel, Brouwers, Nick, de la Torre Cortès, Pilar, Kuijpers, Niels G. A., Daran, Jean-Marc G., Abeel, Thomas“…RESULTS: To fill this gap, we generated a chromosome-level genome assembly of the S. pastorianus strain CBS 1483 from Oxford Nanopore MinION DNA sequencing data and analysed the newly assembled subtelomeric regions and chromosome heterozygosity. …”
Publicado 2019
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703por Szczerba, Hubert, Komoń-Janczara, Elwira, Krawczyk, Mariusz, Dudziak, Karolina, Nowak, Anna, Kuzdraliński, Adam, Waśko, Adam, Targoński, Zdzisław“…Here, we present the first complete genome sequence of this new, wild strain and report its basic genetic features from a biotechnological perspective. The MinION single-molecule nanopore sequencer supported by the Illumina MiSeq platform yielded a circular 5,062,651 bp chromosome with a GC content of 55% that lacked plasmids. …”
Publicado 2020
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704por Gonçalves, Ana Teresa, Collipal-Matamal, Rayen, Valenzuela-Muñoz, Valentina, Nuñez-Acuña, Gustavo, Valenzuela-Miranda, Diego, Gallardo-Escárate, Cristian“…Using third-generation sequencing with Nanopore technology (MinION) the full 16S rRNA gene from sea lice obtained from 8 areas distributed over the three main aquaculture regions were sequenced. …”
Publicado 2020
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705“…Then, to ascertain the origin of the variation in mtDNA content, we used MinION, a nanopore sequencer, to sequence the mitochondrial genomes of H103, SAS, and the tumour spheres. …”
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706por Márquez, Sully, Prado-Vivar, Belén, Guadalupe, Juan José, Gutierrez, Bernardo, Jibaja, Manuel, Tobar, Milton, Mora, Francisco, Gaviria, Juan, García, María, Espinosa, Franklin, Ligña, Edison, Reyes, Jorge, Barragán, Verónica, Rojas-Silva, Patricio, Trueba, Gabriel, Grunauer, Michelle, Cárdenas, Paúl“…In this work we used the MinION platform (Oxford Nanopore Technologies) to sequence the metagenome of the bronchoalveolar lavage (BAL) from this case reported, and subsequently we sequenced the whole genome of the index case and other three patients using the ARTIC network protocols. …”
Publicado 2020
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707por Voorhuijzen, Marleen M., Prins, Theo W., Belter, Anke, Bendiek, Joachim, Brünen-Nieweler, Claudia, van Dijk, Jeroen P., Goerlich, Ottmar, Kok, Esther J., Pickel, Benjamin, Scholtens, Ingrid M. J., Stolz, Andrea, Grohmann, Lutz“…It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. …”
Publicado 2020
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708por Vairo, Francesco, Aimè Coussoud-Mavoungou, Martin Parfait, Ntoumi, Francine, Castilletti, Concetta, Kitembo, Lambert, Haider, Najmul, Carletti, Fabrizio, Colavita, Francesca, Gruber, Cesare E. M., Iannetta, Marco, Messina, Francesco, Lanini, Simone, Ulrich Judicaël, Biez, Giombini, Emanuela, Montaldo, Chiara, Portella, Chantal, Diafouka-Diatela, Steve, Rueca, Martina, Kock, Richard, Bartolini, Barbara, Mboera, Leonard, Munster, Vincent, Fischer, Robert, Seifert, Stephanie, Muñoz-Fontela, César, Escudero-Pérez, Beatriz, Gomez-Medina, Sergio, Nelson, Emily V., Kjia Tungu, Patrick, Nicastri, Emanuele, Puro, Vincenzo, Di Caro, Antonino, Capobianchi, Maria Rosaria, Mikolo, Jacqueline Lydia, Zumla, Alimuddin, Ippolito, Giuseppe“…Full CHIKV genome sequences were obtained by Sanger and MinION approaches and Bayesian tree phylogenetic analysis was performed. …”
Publicado 2020
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709por Moore, Shona C., Penrice-Randal, Rebekah, Alruwaili, Muhannad, Randle, Nadine, Armstrong, Stuart, Hartley, Catherine, Haldenby, Sam, Dong, Xiaofeng, Alrezaihi, Abdulrahman, Almsaud, Mai, Bentley, Eleanor, Clark, Jordan, García-Dorival, Isabel, Gilmore, Paul, Han, Ximeng, Jones, Benjamin, Luu, Lisa, Sharma, Parul, Shawli, Ghada, Sun, Yani, Zhao, Qin, Pullan, Steven T., Carter, Daniel P., Bewley, Kevin, Dunning, Jake, Zhou, En-min, Solomon, Tom, Beadsworth, Michael, Cruise, James, Crook, Derrick W., Matthews, David A., Davidson, Andrew D., Mahmood, Zana, Aljabr, Waleed, Druce, Julian, Vipond, Richard, Ng, Lisa, Renia, Laurent, Openshaw, Peter J. M., Baillie, J. Kenneth, Carroll, Miles W., Stewart, James, Darby, Alistair, Semple, Malcolm, Turtle, Lance, Hiscox, Julian A.“…A long amplicon/read length-based RT-PCR sequencing approach focused on the Oxford Nanopore MinION/GridION platforms was developed to identify and sequence the SARS-CoV-2 genome in samples from patients with or suspected of COVID-19. …”
Publicado 2020
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710por Laternser, Sandra, Cosentino, Chiara Cianciolo, Przystal, Justyna M, Dettwiler, Susanne, Rushing, Elisabeth Jane, Gerber, Nicolas U, Stücklin, Ana Guerreiro, Prasad, Rachna, Grotzer, Michael, Krayenbühl, Niklaus, Müller, Sabine, Nazarian, Javad“…Tumor cells were validated using the minION sequencing platform. Single and combination drug (n=7) screens were performed. …”
Publicado 2020
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711por Girgis, Hany S., DuPai, Cory D., Lund, Jessica, Reeder, Janina, Guillory, Joseph, Durinck, Steffen, Liang, Yuxin, Kaminker, Joshua, Smith, Peter A., Skippington, Elizabeth“…Despite the high potential for misclassification of heterogenous cell populations as either antibiotic-susceptible or fully resistant in clinical settings, and the concomitant risk of inappropriate treatment, CN distribution among cells has defied analysis. Here, we use the MinION single-molecule nanopore sequencer to uncover CN heterogeneity in clonal populations of Escherichia coli and Acinetobacter baumannii grown from single cells isolated while selecting for resistance to an optimized arylomycin, a member of a recently discovered class of Gram-negative antibiotic. …”
Publicado 2021
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712por Zhou, Menglan, Wu, Yarong, Kudinha, Timothy, Jia, Peiyao, Wang, Lei, Xu, Yingchun, Yang, Qiwen“…Bloodstream infection is a major cause of morbidity and mortality worldwide. We explored whether MinION nanopore sequencing could accelerate diagnosis, resistance, and virulence profiling prediction in simulated blood samples and blood cultures. …”
Publicado 2021
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713por Maloney, Jenny G., Jang, Yunah, Molokin, Aleksey, George, Nadja S., Santin, Monica“…To confirm the validity of ST30 and ST31, MinION sequencing was used to obtain full-length SSU rRNA gene sequences, and phylogenetic and pairwise distance analyses were performed. …”
Publicado 2021
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714por Jones, Ashley, Torkel, Cynthia, Stanley, David, Nasim, Jamila, Borevitz, Justin, Schwessinger, Benjamin“…Using these protocols, sequencing on the Oxford Nanopore MinION can achieve read length N50 values of 30–50 kb, with reads exceeding 200 kb and outputs ranging from 15–30 Gbp. …”
Publicado 2021
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715por Stüder, François, Petit, Jean-Louis, Engelen, Stefan, Mendoza-Parra, Marco Antonio“…This strategy relies on (1) the use of the portable MinION DNA sequencer; (2) a DNA barcoding and a SPIKE gene-centered variant’s tracking, increasing the number of candidates per assay; and (3) a real-time diagnostics and variant’s tracking monitoring thanks to our software RETIVAD. …”
Publicado 2021
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716por Jahan, Nusrat A., Lindsey, Laramie L., Kipp, Evan J., Reinschmidt, Adam, Heins, Bradley J., Runck, Amy M., Larsen, Peter A.“…We leveraged the Oxford Nanopore MinION sequencer to provide a rapid real-time survey of putative zoonotic foodborne pathogens, among others. …”
Publicado 2021
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717“…In order to construct the complete genome, high-quality whole-genome sequencing of strain Ni1-3 were performed by using both Nanopore MinION and Illumina NovaSeq platforms, from which the first complete genome of S. decolorationis was obtained by hybrid assembly. …”
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718“…Whole-genome sequencing based on Illumina and MinION platforms showed that NMG38-2 contains 24 acquired antibiotic resistance genes and three plasmids, among which, pNDM_NMG38-2, a pPvSC3-like plasmid, is transferable and co-carries bla(NDM-1) and lnu(G). …”
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719por Hayes, Ben J., Nguyen, Loan T., Forutan, Mehrnush, Engle, Bailey N., Lamb, Harrison J., Copley, James P., Randhawa, Imtiaz A. S., Ross, Elizabeth M.“…Cattle (n = 66) with ages ranging from 0.35 to 15.7 years were sequenced using Oxford Nanopore Technologies MinION and methylation was called at CpG sites across the genome. …”
Publicado 2021
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720por Kirov, Ilya, Polkhovskaya, Ekaterina, Dudnikov, Maxim, Merkulov, Pavel, Vlasova, Anastasia, Karlov, Gennady, Soloviev, Alexander“…We showed that while the target gene enrichment per se was quite high for the three glutenin genes (up to 645×), the sequencing depth that was achieved from two MinION flowcells was relatively low (5–17×). However, this sequencing depth was sufficient for various tasks including detection of InDels and single-nucleotide variations (SNPs), read phasing and methylation profiling. …”
Publicado 2021
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