-
761Identification of pathogens in culture-negative infective endocarditis cases by metagenomic analysispor Cheng, Jun, Hu, Huan, Kang, Yue, Chen, Weizhi, Fang, Wei, Wang, Kaijuan, Zhang, Qian, Fu, Aisi, Zhou, Shuilian, Cheng, Chen, Cao, Qingqing, Wang, Feiyan, Lee, Shela, Zhou, Zhou“…METHODS: To assess the feasibility of a metagenomic approach to detect the causative pathogens in resected valves from IE patients, we employed both next-generation sequencing and Oxford Nanopore Technologies MinION nanopore sequencing for pathogens and antimicrobial resistance detection in seven culture-negative IE patients. …”
Publicado 2018
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
762“…Whole-genome sequencing was performed using both long-read MinION and short-read Illumina platforms to fully characterize the bla(OXA-72)-carrying plasmid of the A. baumannii A52. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
763por Lalaoui, Rym, Djukovic, Ana, Bakour, Sofiane, Hadjadj, Linda, Sanz, Jaime, Salavert, Miguel, López-Hontangas, Jose Luis, Sanz, Miguel A., Ubeda, Carles, Rolain, Jean-Marc“…Whole genome sequencing of C. freundii isolates was performed using the MinION and MiSeq Illumina sequencers. Bioinformatic analysis was performed in order to identify the molecular support of carbapenem resistance and to study the genetic environment of carbapenem resistance encoding genes. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
764“…We also present the efficient completion of circularized M. avium subspecies hominissuis genomes using our extraction technique and the long-read sequencing MinION platform, including the identification of a novel plasmid. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
765por Nguyen, Thu-Phuong, Mühlich, Cornelia, Mohammadin, Setareh, van den Bergh, Erik, Platts, Adrian E., Haas, Fabian B., Rensing, Stefan A., Schranz, M. Eric“…We started with the initially published genome (version 2.5). PacBio and MinION sequencing together with genetic map v2.5 were incorporated to produce the new reference genome v3.0. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
766por Yang, Libing, Haidar, Ghady, Zia, Haris, Nettles, Rachel, Qin, Shulin, Wang, Xiaohong, Shah, Faraaz, Rapport, Sarah F., Charalampous, Themoula, Methé, Barbara, Fitch, Adam, Morris, Alison, McVerry, Bryan J., O’Grady, Justin, Kitsios, Georgios D.“…We collected endotracheal aspirates and applied a microbial DNA enrichment method prior to metagenomic sequencing with the Oxford Nanopore MinION device. For reference, we compared Nanopore results against clinical microbiologic cultures and bacterial 16S rRNA gene sequencing. …”
Publicado 2019
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
767por Cozzuto, Luca, Liu, Huanle, Pryszcz, Leszek P., Pulido, Toni Hermoso, Delgado-Tejedor, Anna, Ponomarenko, Julia, Novoa, Eva Maria“…In a second step, the pipeline performs downstream analyses of the mapped reads, including prediction of RNA modifications and estimation of polyA tail lengths. Four direct RNA MinION sequencing runs can be fully processed and analyzed in 10 h on 100 CPUs. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
768por Dong, Dandan, Mi, Ziqiang, Li, Dujun, Gao, Mingming, Jia, Nan, Li, Manli, Tong, Yigang, Zhang, Xianglilan, Zhu, Yuanqi“…The complete nucleotide sequence of pCRE3-KPC was determined by combining MiSeq and MinION sequencing and then compared with those of three related plasmids. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
769por Zhou, Hua, Larkin, Paige M K, Huang, Jinwei, Yao, Yake, Zhu, Bingquan, Yang, Qing, Hua, Xiaoting, Zhou, Jianying, Yang, Shangxin, Yu, Yunsong“…Whole genome sequencing (WGS) was performed using MinIon and MiSeq. Center for Genomic Epidemiology, CLCbio, Geneious, and Virulence Factors of Pathogenic Bacteria database were used for genomic analysis. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
770por Klimstra, William B., Tilston-Lunel, Natasha L., Nambulli, Sham, Boslett, James, McMillen, Cynthia M., Gilliland, Theron, Dunn, Matthew D., Sun, Chengqun, Wheeler, Sarah E, Wells, Alan, Hartman, Amy L., McElroy, Anita K., Reed, Douglas S., Rennick, Linda J., Duprex, W. Paul“…Two low passage (P) strains of SARS-CoV-2 (Wash1: P4 and Munich: P1) were cultured twice in Vero-E6 cells and characterized virologically. Sanger and MinION sequencing demonstrated significant deletions in the furin cleavage signal of Wash1: P6 and minor variants in the Munich: P3 strain. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
771por Emond-Rheault, Jean-Guillaume, Hamel, Jérémie, Jeukens, Julie, Freschi, Luca, Kukavica-Ibrulj, Irena, Boyle, Brian, Tamber, Sandeep, Malo, Danielle, Franz, Eelco, Burnett, Elton, Daigle, France, Arya, Gitanjali, Sanderson, Kenneth, Wiedmann, Martin, Slawson, Robin M., Weadge, Joel T., Stephan, Roger, Bekal, Sadjia, Gruenheid, Samantha, Goodridge, Lawrence D., Levesque, Roger C.“…Next, to confirm the accuracy of the prediction in 1750 S. enterica short-read sequencing data, we combined Oxford Nanopore MinION long-read sequencing with Illumina MiSeq short-read sequencing in hybrid assemblies for 84 isolates to evaluate the proportion of plasmid that has been detected. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
772por Xanthopoulou, Kyriaki, Urrutikoetxea-Gutiérrez, Mikel, Vidal-Garcia, Matxalen, Diaz de Tuesta del Arco, José-Luis, Sánchez-Urtaza, Sandra, Wille, Julia, Seifert, Harald, Higgins, Paul G., Gallego, Lucía“…Whole genome sequencing (WGS) was completed using the Miseq and MinION platforms, followed by core-genome MLST (cgMLST) and seven-locus MLST analysis. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
773por Oka, Miho, Xu, Liu, Suzuki, Toshihiro, Yoshikawa, Toshiaki, Sakamoto, Hiromi, Uemura, Hayato, Yoshizawa, Akiyasu C., Suzuki, Yutaka, Nakatsura, Tetsuya, Ishihama, Yasushi, Suzuki, Ayako, Seki, Masahide“…This study used a long-read sequencer (MinION) to construct a comprehensive catalog of aberrant splicing isoforms in non-small-cell lung cancers, by which novel isoforms and potential neoantigens are identified. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
774por Xia, Jing, Venkat, Aarthi, Bainbridge, Rachel E., Reese, Michael L., Le Roch, Karine G., Ay, Ferhat, Boyle, Jon P.“…Here, we use the Oxford Nanopore Minion platform to generate near-complete de novo genome assemblies for multiple strains of T. gondii and its near relative, N. caninum. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
775por Li, Lili, Olsen, Rikke Heidemann, Song, Anhua, Xiao, Jian, Wang, Chong, Meng, Hecheng, Shi, Lei“…Whole-genome short and long read sequencing (HiSeq and MinION) results showed that it contained bla(CTX–M–)(55), qnrS1, and tetB genes, with bla(CTX–M–)(55) and qnrS1 located in chromosomal IS26-mediated composite transposon (IS26–qnrS1–IS3–Tn3–orf–bla(CTX–M–)(55)–ISEcp1–IS26). …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
776por Dharmadhikari, Tanmay, Rajput, Vinay, Yadav, Rakeshkumar, Boargaonkar, Radhika, Patil, Dhawal, Kale, Saurabh, Kamble, Sanjay P., Dastager, Syed G., Dharne, Mahesh S.“…We conducted study in open wastewater drains from December 2020–March 2021 to assess the presence of SARS-CoV-2 nucleic acid and further detect mutations using ARTIC protocol of MinION sequencing. The analysis revealed 108 mutations across six samples categorised into 39 types of mutations. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
777por Liepa, Rebekah, Mann, Riti, Osman, Marwan, Hamze, Monzer, Gunawan, Cindy, Hamidian, Mohammad“…The whole-genome sequence of Cl415 was determined using a combination of the Illumina MiSeq and Oxford Nanopore (MinION) platforms. Complete genome was assembled using Unicycler and antibiotic resistance determinants and ISs were identified using ResFinder and ISFinder, respectively. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
778por Steinig, Eike, Duchêne, Sebastián, Aglua, Izzard, Greenhill, Andrew, Ford, Rebecca, Yoannes, Mition, Jaworski, Jan, Drekore, Jimmy, Urakoko, Bohu, Poka, Harry, Wurr, Clive, Ebos, Eri, Nangen, David, Manning, Laurens, Laman, Moses, Firth, Cadhla, Smith, Simon, Pomat, William, Tong, Steven Y C, Coin, Lachlan, McBryde, Emma, Horwood, Paul“…Successive barcoded panels of S. aureus isolates (2 × 12 per MinION) sequenced at low coverage (>5× to 10×) provided sufficient data to accurately infer genotypes with high recall when compared with Illumina references. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
779por Pallerla, Srinivas Reddy, Van Dong, Do, Linh, Le Thi Kieu, Van Son, Trinh, Quyen, Dao Thanh, Hoan, Phan Quoc, Trung, Ngo Tat, The, Nguyen Trong, Rüter, Jule, Boutin, Sébastien, Nurjadi, Dennis, Sy, Bui Tien, Kremsner, Peter G., Meyer, Christian G., Song, Le Huu, Velavan, Thirumalaisamy P.“…The isolated DNA was subjected to 16S sequencing using MinION™. The data were analysed in real time via the EPI2ME cloud platform. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
780por Senthakumaran, Thulasika, Moen, Aina E. F., Tannæs, Tone M., Endres, Alexander, Brackmann, Stephan A., Rounge, Trine B., Bemanian, Vahid, Tunsjø, Hege S.“…Further, Fusobacterium positive tumor biopsies were subjected to MinION nanopore sequencing of Fusobacterium-specific amplicons to characterize the Fusobacterium species and subspecies. …”
Publicado 2023
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto