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61por Brown, Scott D., Dreolini, Lisa, Wilson, Jessica F., Balasundaram, Miruna, Holt, Robert A.“…RESULTS: Here, we describe a cost-effective and accurate plasmid sequencing and consensus generation procedure using the Oxford Nanopore Technologies’ MinION device as an alternative to capillary-based plasmid sequencing options. …”
Publicado 2023
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62por Cha, Teahyen, Kim, Hoo Hugo, Keum, Jihyun, Kwak, Min-Jin, Park, Jae Yong, Hoh, Jeong Kyu, Kim, Chang-Ryul, Jeon, Byong-Hun, Park, Hyun-KyungEnlace del recurso
Publicado 2023
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63“…Portable DNA sequencers such as the Oxford Nanopore MinION device have the potential to be truly disruptive technologies, facilitating new approaches and analyses and, in some cases, taking sequencing out of the lab and into the field. …”
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64por Oikonomopoulos, Spyros, Wang, Yu Chang, Djambazian, Haig, Badescu, Dunarel, Ragoussis, Jiannis“…To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix were sequenced. …”
Publicado 2016
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65por Minervini, Crescenzio Francesco, Cumbo, Cosimo, Orsini, Paola, Brunetti, Claudia, Anelli, Luisa, Zagaria, Antonella, Minervini, Angela, Casieri, Paola, Coccaro, Nicoletta, Tota, Giuseppina, Impera, Luciana, Giordano, Annamaria, Specchia, Giorgina, Albano, Francesco“…Oxford Nanopore Technologies recently released the MinION an USB-interfaced sequencer. In this paper we report our experience, with the MinION technology for the detection of the TP53 gene mutation in CLL patients. …”
Publicado 2016
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66“…The MinION device by Oxford Nanopore produces very long reads (reads over 100 kBp were reported); however it suffers from high sequencing error rate. …”
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67por Ludden, Catherine, Reuter, Sandra, Judge, Kim, Gouliouris, Theodore, Blane, Beth, Coll, Francesc, Naydenova, Plamena, Hunt, Martin, Tracey, Alan, Hopkins, Katie L., Brown, Nicholas M., Woodford, Neil, Parkhill, Julian, Peacock, Sharon J.“…Small, portable sequencing instruments such as the MinION have the potential to improve the quality of information gathered on antimicrobial resistance in the environment.…”
Publicado 2017
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68por Eccles, David, Chandler, Jodie, Camberis, Mali, Henrissat, Bernard, Koren, Sergey, Le Gros, Graham, Ewbank, Jonathan J.“…RESULTS: Using Nippostrongylus brasiliensis as a test case, applying improved base-calling algorithms and assembly methods, we demonstrate the feasibility of de novo genome assembly matching current community standards using only MinION long reads. In doing so, we uncovered an unexpected diversity of very long and complex DNA sequences repeated throughout the N. brasiliensis genome, including massive tandem repeats of tRNA genes. …”
Publicado 2018
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69por Tyson, John R., O'Neil, Nigel J., Jain, Miten, Olsen, Hugh E., Hieter, Philip, Snutch, Terrance P.“…The Oxford Nanopore Technologies MinION is a portable device that uses nanopore technology that can directly sequence DNA molecules. …”
Publicado 2018
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70por Tyler, Andrea D., Mataseje, Laura, Urfano, Chantel J., Schmidt, Lisa, Antonation, Kym S., Mulvey, Michael R., Corbett, Cindi R.“…The MinION sequencer (Oxford Nanopore Technologies) is a paradigm shifting device allowing rapid, real time long read sequencing of nucleic acids. …”
Publicado 2018
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71“…Assemblies using only MiSeq reads were highly accurate but lacked contiguity, a deficiency that was partially overcome by adding MinION reads to these assemblies. Even more contiguous genome assemblies were generated by using MinION reads for initial assembly, but these assemblies were more error-prone and required further polishing. …”
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72por Leija‐Salazar, Melissa, Sedlazeck, Fritz J., Toffoli, Marco, Mullin, Stephen, Mokretar, Katya, Athanasopoulou, Maria, Donald, Aimee, Sharma, Reena, Hughes, Derralynn, Schapira, Anthony H.V., Proukakis, Christos“…METHODS: We sequenced GBA on the Oxford Nanopore MinION as an 8.9 kb amplicon from 102 individuals, including patients with Parkinson's and Gaucher diseases. …”
Publicado 2019
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73por Deshpande, Samir V., Reed, Timothy M., Sullivan, Raymond F., Kerkhof, Lee J., Beigel, Keith M., Wade, Mary M.“…Field laboratories interested in using the MinION often need the internet to perform sample analysis. …”
Publicado 2019
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74por Imai, Kazuo, Nemoto, Rina, Kodana, Masahiro, Tarumoto, Norihito, Sakai, Jun, Kawamura, Toru, Ikebuchi, Kenji, Mitsutake, Kotaro, Murakami, Takashi, Maesaki, Shigefumi, Fujiwara, Taku, Hayakawa, Satoshi, Hoshino, Tomonori, Seki, Mitsuko, Maeda, Takuya“…The practical advantage of whole genome analysis using the MinION nanopore sequencer is that it can aid in MGS surveillance. …”
Publicado 2020
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75por Ye, Fuqiang, Han, Yifang, Zhu, Juanjuan, Li, Peng, Zhang, Qi, Lin, Yanfeng, Wang, Taiwu, Lv, Heng, Wang, Changjun, Wang, Chunhui, Zhang, Jinhai“…A genome sequence assembled from MinION reads was further classified as HAdV subtype 21a. …”
Publicado 2020
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76por Davidov, Keren, Iankelevich-Kounio, Evgenia, Yakovenko, Iryna, Koucherov, Yuri, Rubin-Blum, Maxim, Oren, Matan“…The taxonomic barcodes were sequenced using Oxford Nanopore Technology with multiplexing on a single MinION flow cell. We identified between 1249 and 2141 species in each of the plastic samples, of which 61 species (34 bacteria and 27 eukaryotes) were categorized as plastic-specific, including species that belong to known hydrocarbon-degrading genera. …”
Publicado 2020
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77“…Furthermore, CODEHOP primers were more effective than standard primers for the detection of minority resistant variants by MinION sequencing.…”
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78por Liao, Yu-Chieh, Wu, Han-Chieh, Liou, Ci-Hong, Lauderdale, Tsai-Ling Yang, Huang, I-Wen, Lai, Jui-Fen, Chen, Feng-Jui“…However, the advantages of the MinION device are often overshadowed by its lower raw accuracy. …”
Publicado 2022
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79por Whitford, Whitney, Hawkins, Victoria, Moodley, Kriebashne S., Grant, Matthew J., Lehnert, Klaus, Snell, Russell G., Jacobsen, Jessie C.“…The application of Oxford Nanopore Technologies’ MinION sequencer has the potential to fulfil this requirement. …”
Publicado 2022
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80por Mgwatyu, Yamkela, Cornelissen, Stephanie, van Heusden, Peter, Stander, Allison, Ranketse, Mary, Hesse, Uljana“…Long-read sequencing is becoming standard procedure for plant genome research, as these reads can span repetitive regions of the DNA, substantially facilitating reassembly of a contiguous genome. With the MinION, Oxford Nanopore offers a cost-efficient sequencing method to generate long reads; however, DNA purification protocols must be adapted for each plant species to generate ultra-pure DNA, essential for these analyses. …”
Publicado 2022
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